Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Countercurrent Distribution”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 217 records · Page 12Linked to original sources
PubMed1993

[Variation of behavioral traits in natural Drosophila melanogaster populations from Eurasia].

Two behavior traits--phototactic response and locomotor activity--were studied in 13 populations of Drosophila melanogaster with countercurrent distribution technique. These traits were characterized by three parameters: average phototactic (locomotor) activity, normalized variance of activity and proportion of inactive flies. Males had bigger average phototactic activity and average locomotor activity, but less proportion of inactive flies than females. For phototaxis, no correlation was found between all estimated parameters and geographic localization of populations. For locomotor activity, females exhibited clinal variation in proportion of inactive flies.

Animals↗
PubMed1984

An apparatus for counter-current distribution in a centrifugal acceleration field.

An apparatus is described that reduces the time required for counter-current distribution in aqueous polymer two-phase systems by a factor of more than five. Phase settling, which has been the time consuming step, is here facilitated by centrifugation. The crucial point in the construction, that allows automatization, is that the transfer step in the counter-current distribution cycle is performed during centrifugation.

Centrifugation↗
PubMed1987

Separation by counter-current distribution of young-chick erythrocyte populations and numerical resolution of distribution curves.

The presence of two red cell populations in young chicks has been demonstrated after increasing the settling time used during counter-current distribution with charged 5% Dextran--4% poly(ethylene glycol) two-phase systems. A Fortran program using statistical methods was applied to show the resolution in two peaks (with two or three subpopulations assumed) of the counter-current distribution curves.

Animals↗
PubMed1986

Fractionation of rat liver plasma-membrane regions by two-phase partitioning.

Rat liver plasma membranes, enriched in blood-sinusoidal or bile-canalicular regions by differential and sucrose-gradient centrifugation, were further purified by partitioning in an aqueous polymer two-phase system. This method separates membranes according to differences in surface properties rather than size and density. A several-fold increase in the ratio of leucine aminopeptidase (a bile-canalicular marker) and 5'-nucleotidase to asialo-orosomucoid binding (a blood-sinusoidal marker) was obtained in one fraction, whereas another fraction gave a 2-3-fold increase in ratio of blood-sinusoidal to bile-canalicular markers. Furthermore, the markers for both regions of the plasma membrane, as well as markers for Golgi membranes and lysosomes, showed a heterogeneous behaviour on counter-current distribution.

Animals↗