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Statistical assessment of population variability: a methodological approach.

We have implemented a multivariate statistical methodology to assess the degree and pattern of cranial variability in skeletal samples. Specifically, the method is designed to test whether variability in a skeletal sample exceeds "normal within-group variability" defined in the present instance as variability present among crania from a cemetery associated with a village. It involves comparing a covariance matrix derived from a sample of unknown composition to one representing "normal within-group variability." The method has been applied to two Plains Indian craniometric samples. The Leavenworth site (39CO9) represents the remnants of previously autonomous Arikara Indian villages devastated by epidemic diseases in the late 1700s. The Bad River 2 Phase is an archaeological designation grouping together closely related sites in the Bad-Cheyenne region of South Dakota dating from 1740-1795 AD. We were able to show substantial heterogeneity among crania from Leavenworth. District burial areas at Leavenworth account for some of the heterogeneity, supporting the notion that they represent an attempt to maintain former social distinctions. We were unable to differentiate among sites within the Bad River 2 Phase, suggesting that it is a valid biological unit.

Analysis of Variance↗

Paleodemography of the Carlston Annis (Bt-5) Late Archaic skeletal population.

The study presents a demographic assessment of the Carlston Annis (Bt-5) Late Archaic hunting and gathering population recovered from the banks of the Green River in west-central Kentucky. The shell midden habitation and cemetery site originally yielded the remains of 390 individuals. Radiocarbon dates place site occupation between 3,000 and 4,500 y.b.p. The skeletal sample consisted of 354 individuals ranging in age from 7 months in utero to 70 + years. Subadults were aged by seriation of dental and skeletal developmental criteria. Adult ages were determined by the multifactorial summary age method that employed 1) five indicators of adult skeletal age at death, 2) the procedure of age indicator seriation, and 3) differential weighting of age assessments as determined by principal components analysis. Adult sex diagnoses were based on qualitative assessment of pelvic and cranial morphological criteria. The Bt-5 life table analysis yields an E0 of 22.4 years, crude birth of 45, mean family size of 3.3, gross reproductive rate of 2.7, generation length of 26.6 years, and B of .076, indicating a healthy population with a substantial capability to replace succeeding generations. Survivorship profiles and demographic parameters that compare the Carlston Annis (Bt-5) and Indian Knoll (Oh-2) skeletal series are presented. Both populations display type II survivorship curves, with high infant mortality and early onset of elevated mortality rates in adults. Major differences between Bt-5 and Oh-2 demographic parameters concern adult sex ratio and adult age distribution over 30 years. These differences are interpreted to reflect census errors in the Oh-2 demographic reconstruction that were possibly introduced by selective methodological biases and/or taphonomic factors.

Age Determination by Skeleton↗

Histologic estimation of age at death using the anterior cortex of the femur.

This report describes a new histologic method for determination of age at death, the latest in a series of studies that began with Kerley's pioneer presentation in 1965. The study population was collected from 328 documented individuals from an anatomy dissecting room in the United States, from two modern cemeteries in the Dominican Republic, and from autopsies performed in a Chilean hospital. Undecalcified thin sections 1.0 cm wide were made from specimens taken from the femoral midshaft directly opposite the linea aspera. Five 0.886 mm2 fields were located at the periosteal edge and photographed, mainly for purposes of defining the fields and providing a permanent record. Secondary osteons, type II osteons, osteon fragments, resorption spaces, and non-Haversian canals were recorded as number/mm2, and a 100-space grid was used to measure average percent of unremodeled, osteonal, and fragmental bone. Stepwise regression analysis of the measurements produced a series of regression equations for age estimation for females, males, and sexes combined. Most equations have a standard error of estimate of about 10 years, but the coefficients of determination (r2) range from 0.48 to 0.72. In practice, sex-specific equations gave better results than opposite-sex or nonspecific equations, mainly because males and females differed in the pattern of relations between osteons and osteon fragments with advancing age.

Adolescent↗

Histologic examination of age of the First African Baptist Church adults.

This study applies a recently formulated method of histologic estimation of age at death to samples of anterior femoral cortex taken from adult skeletons from the First African Baptist Church cemetery and compares the results with macroscopic age estimates made by J.L. Angel and J.O. Kelley. Of the 65 samples taken, 44 were sufficiently well preserved to produce readable thin sections, although most were so fragile that they had to be embedded before sectioning. In most cases the histologic ages agreed with the macroscopic age estimations within the standard error of the estimate of the histologic method. Most cases of disagreement could be attributed to poor preservation. Despite differences between individual gross and microscopic age estimates, the two methods proved to be significantly correlated and produced the same overall demographic picture of a population whose female members were likely to die at earlier ages than the males.

Age Determination by Skeleton↗

Stature in an early mid-19th century poorhouse population: Highland Park, Rochester, New York.

If the early 19th century United States was a developing country, then it may be expected that the lowest economic stratum would show some biological consequences of poverty. This report examines that question by estimating stature on 90 male and 64 female adult skeletons from an unmarked cemetery dating between 1826 and 1863. The Highland Park burial ground was adjacent to institutions which interned unfortunates of Rochester, Monroe County, in western New York. The best estimate of male stature (N = 84) is 172.6 cm. A review of other 18th and 19th century height data shows this value not to be relatively stunted, but rather exactly on the predicted mean of its time. If this was a poor population, stunting was not a consequence of poverty. Females (N = 59) showed a best height estimate of 160.0 cm. Less comparative data are available, but this too seems average for the time. A brief discussion of secular trends, changing income inequity and fluctuating stature from the 18th through 20th centuries puts this finding in context.

Body Height↗

Congenital syphilis in the past: slaves at Newton Plantation, Barbados, West Indies.

Hutchinson's incisors and Moon's molars are specific lesions of congenital syphilis. The extensive but fragmentary clinical literature on these conditions describes reduced dimensions and thin enamel in the permanent incisors and first molars, crowding and infolding of the first molar cusps, notching of the upper incisors, and apical hypoplasias of the permanent canines. A Barbados slave cemetery (ca. 1660-1820 AD) includes three individuals with these features, suggesting a frequency at birth of congenital syphilis in the population approaching 10%. These three cases show triple the frequency of all hypoplasias and more than seven times the frequency of pitting hypoplasia present in the remainder of the series. The recognizable congenital syphilis cases account for much of the remarkably high frequency of hypoplasias in the series as a whole. We infer that syphilis contributed substantially to morbidity, infant mortality, and infertility in this population. Presence or absence of congenital syphilis may account for much of the variability in health and mortality seen among nineteenth century African-American populations.

Adolescent↗

Sex determination of infant and juvenile skeletons: I. Morphognostic features.

Ancient cemeteries are often characterized by a considerable number of infants and young children. Sex differences in childhood mortality, however, could rarely be studied up to now, mainly because there were only few proven traits for sexual determination of immature skeletons. Based on a historic sample of sixty-one children of known sex and age from Spitalfields, London (37 boys, 24 girls), sexually distinctive traits in the mandible and ilium are presented for morphognostic diagnosis. Besides other features, boys typically show a more prominent chin, an anteriorly wider dental arcade, and a narrower and deeper sciatic notch than girls. Most of the traits presented in this study allow individuals between birth and five years of age to be successfully allocated to either sex in 70-90% of the cases.

Age Factors↗

Paleoserology of the Christian population at Sayala (Lower Nubia): an evaluation of the reliability of the results.

A paleoserological study of human remains from the Christian cemeteries of Sayala (Lower Nubia, 6th-11th centuries A.D.) was carried out by applying two techniques (absorption-elution and haemagglutination-inhibition), on two types of substratum (bones and hair), in separate laboratories (Pisa and Prague). The aim of research was to evaluate the degree of repeatability of the results and the reliability of the final paleoserological diagnoses. The results indicated different degrees of repeatability depending on the differential sensitivity of the two techniques and on the nature of the substratum. The frequencies of concordant and discordant final diagnoses within and between the two laboratories and by the two methods were significantly different from those expected under the conditions of random distribution, indicating a consistency in results from different laboratories and methods. The reliability of the resulting gene and phenotype distributions is also supported by their consistency both with the theoretical Hardy-Weinberg equilibrium and with the present distribution of the ABO gene frequencies in Egyptian, Sudanese, and Nubian populations.

Blood Grouping and Crossmatching↗

Human dento-gnathic metric variation in mesolithic/neolithic Ukraine: possible evidence of demic diffusion in the Dnieper Rapids region.

Dental and gnathic metrics from a series of Mesolithic and Neolithic cemetery samples in the Dnieper River valley (Ukraine) are compared. Both male and female Neolithic samples have larger dental dimensions, wider dental arcades, and a more robust mandibular corpus than do the Mesolithic samples. In addition, the relative variances (RVs) of bucco-lingual dental breadths (as measured by modified Levene's Tests) show an intriguing pattern of change from the Mesolithic into and through the Neolithic. Female RVs show a clear tenency to increase through time, while male RVs show more mixed tendencies. Such a pattern indicates that the increases in Ukrainian Neolithic dento-gnathic dimensions are plausibly attributable to low intensity gene flow (demic diffusion). Seen in the light of new chronometric, paleodietary, and paleolinguistic information, as well as in the context of recent archaeological models for agro-pastoralist origins in the North Pontic, these data suggest that gene flow via population interactions originating in or transient through the circum-Caucasus may have played an important role in producing the Ukrainian Neolithic dental increase.

Anthropology, Physical↗

Frequency and chronological distribution of dental enamel hypoplasia in enslaved African Americans: a test of the weaning hypothesis.

The dentition of 27 enslaved African Americans from archaeological sites in Maryland and Virginia were examined. All 17 males and 7 of the 10 females in this study exhibited enamel hypoplastic defects indicative of systemic nutritional and disease stresses interfering with amelogenesis. Estimates of the ages of occurrence of these defects show that most occur between 1.5 and 4.5 years of age, 0.5-3.75 years later than historically documented weaning age (9-12 months of age) in similar plantation populations. Comparisons are made with studies of dental enamel hypoplasia in contemporaneous enslaved and free African American populations, including our data on 75 individuals from the First African Baptist Church cemetery in Philadelphia. These populations were highly stressed. While there appears to be a modest effect of early weaning stress, no direct relationship of peak frequencies to weaning age can be shown. These data raise questions about the attribution of peak hypoplasia frequencies to age at weaning or "post-weaning" stresses in previous paleopathological studies. High hypoplasia frequencies during the middle years of enamel development are more likely the result of a combination of 1) multiple environmental stresses, 2) differences in hypoplastic susceptibility in enamel, and 3) random factors.

Black or African American↗

Continuity or colonization in Anglo-Saxon England? Isotope evidence for mobility, subsistence practice, and status at West Heslerton.

The adventus Saxonum is a crucial event in English protohistory. Scholars from a range of disciplines dispute the scale and demographic profile of the purported colonizing population. The 5th-7th century burial ground at West Heslerton, North Yorkshire, is one of the few Anglian cemeteries where an associated settlement site has been identified and subjected to extensive multidisciplinary postexcavation study. Skeletal and grave good evidence has been used to indicate the presence of Scandinavian settlers. A small, preliminary study using lead and strontium isotope analysis of tooth enamel, mineralized in early childhood, from Neolithic/Early Bronze Age (n = 8), Iron Age (n = 2), and Early Anglo-Saxon (n = 32) skeletons, was carried out to directly investigate this hypothesis. Results suggest that lead provides dissimilar types of information in different time periods. In post-Roman England, it appears to reflect the level of exposure to circulated anthropogenic rather than natural geological lead, thus being a cultural rather than geographical marker. Consequently, only strontium provides mobility evidence among the Anglian population, whereas both isotope systems do so in pre-Roman periods. Strontium data imply the presence of two groups: one of "local" and one of "nonlocal" origin, but more work is required to define the limits of local variation and identify immigrants with confidence. Correlations with traditional archaeological evidence are inconclusive. While the majority of juveniles and prehistoric individuals fall within the "local" group, both groups contain juveniles, and adults of both sexes. There is thus no clear support for the exclusively male, military-elite invasion model at this site.

Dental Enamel↗

Brief communication: the collection of identified human skeletons housed at the Bocage Museum (National Museum of Natural History), Lisbon, Portugal.

A large collection of identified human skeletons curated at the Bocage Museum (National Museum of Natural History, Lisbon, Portugal) has remained in relative anonymity since its collecting protocol was initiated in the 1980s. This collection originates from modern cemetery sources and is comprised of 1,692 skeletons with basic documentary data (age at death, place of birth, occupation, place of residence, and date and cause of death). At present, this information is more readily available for 699 individuals. The remaining 993 are in the process of being fully documented. The skeletons consist largely of Portuguese nationals who lived in the 19th and 20th centuries (1805-1975) in Lisbon. Both sexes are equally represented, and ages at death range from birth to 98 years, including 92 subadults (<20 years old).

Female↗

Isotopic evidence for breastfeeding and possible adult dietary differences from Late/Sub-Roman Britain.

Historical documents indicate that breastfeeding and weaning practices have fluctuated in England through history. In order to obtain evidence for general breastfeeding patterns in Late/Sub-Roman Britain, stable carbon and nitrogen isotope values were measured in juvenile and adult skeletons (n = 87) from the cemetery of Queenford Farm, Dorchester-on-Thames, Oxfordshire. As the site contained few individuals between 0-1.5 years of age, it was not possible to determine the initial timing for the introduction of weaning foods. Between ages 2-4 years, the mean +/- SD delta(13)C results (-20.2 +/- 0.3 per thousand) are significantly more negative (t = -4.03, P < 0.001) compared to adult females (-19.7 +/- 0.3 per thousand). This is interpreted as evidence of a different diet being fed to children during weaning. After age 2, the delta(15)N values gradually decline, indicating complete cessation of breastfeeding by 3-4 years. Among adults, stature (males = 1.68 +/- 0.06 m; females = 1.58 +/- 0.07 m) and sexual dimorphism (106) were low, suggesting that the population was possibly under environmental stress. The delta(13)C results for adults are similar, but females show a small but statistically significantly (t = -2.86, P < 0.01) lower mean delta(15)N value (9.9 +/- 0.9 per thousand) compared to males (10.6 +/- 0.5 per thousand). These lower female delta(15)N values possibly reflect the different physiology of the sexes (pregnancy and/or lactation) or the reduced consumption of animal/fish protein by women, and this may have been influenced by individual preference, family needs, or societal values of the era.

Adult↗

Skeletal manifestations of infantile scurvy.

Recent investigations of human skeletal material from the historic St. Martin's cemetery, England, found a range of abnormal lesions in six infants that are almost certainly related to scurvy. Porous and proliferative bone lesions affecting the cranial bones and scapulae were found, and this paper presents images obtained using both macroscopic and scanning electron microscope examination of the lesions. Previous work on infantile scurvy (Ortner et al., 1997-2001) relied heavily on changes at the sphenoid, which is often missing in archaeological bone, so the identification of changes attributable to scurvy on other cranial bones and the scapulae is encouraging. The ability to recognize changes related to scurvy on a range of bones will ensure an enhanced potential for recognition of this disease in future research involving archaeological bone. Research on historical documents from Birmingham dating to the eighteenth and nineteenth centuries, combined with the probable cases of scurvy identified, supports the view that the paucity of cases of infantile scurvy from the archaeological record reflects a lack of understanding and recognition of bone manifestations, rather than a lack of occurrence in this period. Changes linked to scurvy were only found in infants from the poorer sections of the community from St. Martin's, and this is almost certainly linked to patterns of food consumption and may be related to shortages of potatoes, due to blight, experienced during this period.

Bone and Bones↗

Health of the non-elites at Tombos: Nutritional and disease stress in New Kingdom Nubia.

During the New Kingdom period, Egypt succeeded in occupying most of Nubia. Colonial towns were built, which served as centers of government and redistribution. This paper uses a bioarchaeological approach to address the effects of this cultural contact on non-elites. Skeletal remains from the site of Tombos (N = 100), a cemetery in Upper Nubia dating to this important time, are analyzed, in addition to 1,082 individuals from contemporaneous Egyptian and Nubian sites, in order to shed light on the social, political, and economic processes at play and to determine how the people at Tombos were affected during this transitional period. In many ways, the Tombos population appears to have been affected by similar stressors as the other populations under study. However, a few small differences in the subadult frequencies of pathological lesions, especially remodeling rates, are significant in the overall picture of health at Tombos. These analyses suggest that, although the people of Tombos may have been integrated into the Egyptian colonial network, the additional resources they may have obtained could not protect them from nutritional and disease stress. A lower childhood survival through bouts of ill health at Tombos is suggested. While status may have played a role in the differences seen in the comparative populations, it is likely that parasites and/or other infections led to childhood illness and death.

Adolescent↗

Cortical bone remodeling rates in a sample of African American and European American descent groups from the American Midwest: comparisons of age and sex in ribs.

This study employs regression analysis to explore population and sex differences in the pattern of age-associated bone loss, as reflected by histomorphometric variables that are measures of intracortical and endocortical bone remodeling. A comparison of an African American sample from the Washington Park Cemetery in St. Louis, Missouri, and a European American rib sample composed of cadavers, autopsies, and forensic cases from Missouri reveals the existence of complex age-associated patterns for differences in measures of intracortical remodeling and cortical area. Females from the two samples express similar bone dimensions and dynamics. The African American females appear to lose more bone than their male counterparts, but this difference is absent in the European American sample. When age-associated patterns are considered, it is in the younger cohorts that African Americans exhibit greater relative cortical area than European Americans, but this is reversed in the older ages, when the latter group manifests greater bone mass. The European American males consistently differ in the slopes and intercepts for the variables compared to the other groups, and differences are highly significant with African American females, with the former group maintaining bone mass while the latter exhibit a more rapid bone loss. Achieving larger relative cortical area due to smaller endosteal area, coupled with better bone quality due to lower intracortical porosity early in life, may be a mechanism by which African Americans, especially females, maintain adequate bone mass in older ages, which buffers them from bone loss and related fragility fractures despite higher rates of intracortical remodeling and endosteal expansion later in life. These results suggest that both genetic and environmental factors are responsible for the differences in bone remodeling and bone mass observed between these samples.

Adolescent↗

Osteoporosis in a population from medieval Norway.

Modern populations from Norway and England differ in their experience of osteoporosis, the former showing lower bone mineral density (BMD) and a higher fragility fracture rate. The aim of the present work was to investigate whether this was also the case during the Middle Ages. Age-dependent loss of BMD in the proximal femur was assessed using dual X-ray absorptiometry (DXA) in male and female adult skeletons from a cemetery in the medieval town of Trondheim, Norway. Fracture prevalence was also investigated. Results were compared with those previously reported for a skeletal series from Wharram Percy, a deserted medieval village in England. Results indicate that peak BMD and patterns of age-related loss of BMD in the Norwegian and the English group were similar. Among females, the prevalence of osteoporotic fractures was greater in the Norwegian than in the English population. The BMD results suggest that differences in BMD between English and Norwegians are of recent origin, although given the fairly modest sample sizes, further work is needed to confirm this. Reasons for the greater prevalence of osteoporotic fractures in women in the Norwegian skeletal series are unclear, but the colder climate and greater frequency of hard surfaces may have meant that falls were more frequent, and when they occurred, were more likely to result in fractures than in the rural English group.

Adolescent↗

Molecular genetic analysis of Wanggu remains, Inner Mongolia, China.

The Wanggu tribe, which contributed significantly to the foundation of the Yuan Dynasty, was one of the groups living on the Mongolian steppes during the Jin-Yuan period (AD 1127-1368) of Chinese history. However, there has been both archaeological and historical dispute regarding the origin of the ancient tribe. Recently, we discovered human remains of the Wanggu tribe in the Chengbozi cemetery in the Siziwang Banner of Inner Mongolia, China. To investigate the genetic structure of the Wanggu tribe and to trace the origins of the tribe at a molecular level, we analyzed the control-region sequences and coding regions of mitochondrial DNA (mtDNA) from the remains by direct sequencing and restriction-fragment length polymorphism analysis. In combination with mtDNA data of 15 extant Eurasian populations, we performed phylogenetic analysis and multidimensional scaling analysis. Our results show that the genetic structure of the Wanggu tribe in the Jin-Yuan period is a complex matriline, containing admixture from both Asian and European populations. In addition, we reveal that on the basis of mtDNA data, the ancient tribe may share a recent common ancestor with the Turkic-speaking Uzbeks and Uighurs.

Adolescent↗