Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “CONGO”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 217 records · Page 12Linked to original sources

Congo red staining on 1 micron de-plasticized sections for detection of lesions in Alzheimer's disease and related disorders.

Neuritic plaques (NPs), neurofibrillary tangles (NFTs) and congophilic angiopathy (CA), the three characteristic lesions in Alzheimer's disease, are easily detected in paraffin sections using light microscopy and specific staining methods including Congo red and Thioflavin S. Identification of these lesions in plastic thick sections (1 micron) is more tedious and relies essentially on morphological criteria. This causes investigators to subsequently analyze large numbers of thin sections under the electron microscope. Since many researchers use electron microscopy for various aspects of Alzheimer's disease and related research, it would be advantageous to have a rapid method enabling the investigator to quickly and reliably identify in thick sections the characteristic NPs, NFTs and/or CA, which can then be used for further analysis at the ultrastructural level. In this context, the present study describes a dependable technique for identifying NPs, NFTs and/or CA in Alzheimer's disease and related disorders and involves Congo red staining on one micron sections after plastic removal.

Aged↗

Unusual transferrin TF*D mutants in some Congo populations.

Transferrin allele (TF) distribution was investigated in three Bantu and one Babenga Pygmy groups of Congo, by isoelectric focusing on polyacrylamide (PIEF). In comparison with other Black populations, the four groups had a twofold higher TF*D frequency. Three different TF*D mutants were observed, the commonest type of which was identical by PIEF to a *D1 Caucasian allele. The classification of the other two is discussed. The homozygous TF D1 pattern was present in Babenga. The polymorphism of transferrin confirmed the uniqueness of anthropological traits in such Congo populations.

Alleles↗

Aberrant development of Trichophyton mentagrophytes hyphae cultured in the presence of Congo red.

When Trichophyton mentagrophytes colonies were placed on a medium containing 150 micrograms/ml of Congo red, a dye which prevents chitin fibrillogenesis, their growth rate was reduced. The newly formed mycelium, examined under an ultraviolet microscope, consisted of thick, curled and branched hyphae endowed with swollen tips and subapical bulges. Short-time exposure revealed that the major sites of dye accumulation were the extension zones. Normally structured hyphae arose from aberrant mycelia when they were transferred onto a dye-free medium. The phenomena observed suggest that Congo red alters the wall properties of the extension zone, by inhibiting the gradual conversion of chitin chains, synthesized at the extreme tip, into microfibrils of increasing size and density.

Congo Red↗

Epidemiological study of the relationship between Congo red binding Escherichia coli and avian colisepticemia.

An epidemiological prospective (longitudinal) study design was used to evaluate the association of Congo red positive Escherichia coli and avian colisepticemia. High and low risk exposure groups of chickens were identified at hatching, and placed in separate identical houses on the same farm. Approximately 14,000 birds were placed in each house for the seven week grow-out period, during which all birds which died were necropsied and cultured, together with a representative sample of birds which were culled weekly. The findings implicated Escherichia coli as the etiological agent of avian colisepticemia. A relative risk of 6.5 and attributable risk of 73.5% supported the hypothesis that the Congo red medium identifies a virulent form of Escherichia coli which causes airsacculitis-colisepticemia in poultry.

Air Sacs↗

Congo red medium to distinguish between invasive and non-invasive Escherichia coli pathogenic for poultry.

In the course of our molecular studies of virulence factors associated with invasive avian Escherichia coli infections, it was first necessary to distinguish between common E. coli and those that cause septicemia in poultry. We found a direct correlation between the ability of clinical isolates of E. coli to bind Congo red dye (CR) and their ability to cause septicemic infection in chickens. This finding was supported by bacteriological studies of 30 broiler flocks (26 sick and 4 healthy) and by virulence studies in chickens and mice. All 144 isolates of E. coli from internal tissues of diseased birds were determined to be CR-positive (red colonies). Congo-red-positive E. coli colonies were isolated from air sacs, pericardium, liver, lung, joint fluid, and heart blood of chickens with lesions of colisepticemia. In contrast, of 170 E. coli isolates from the poultry house environment and from the trachea and cloaca of healthy birds, more than half were CR-negative (white colonies). No CR-negative (white) E. coli colonies were found in internal organs from birds with typical lesions of colisepticemia. We feel that these preliminary findings suggest that the CR dye binding could be used as a phenotypic marker to distinguish between invasive and noninvasive isolates.

Animals↗

Exocytosis in Saccharomyces cerevisiae treated with congo red.

When dividing cells of Saccharomyces cerevisiae were exposed to the polysaccharide-binding dye Congo red, the walls and septa became sites of chitin accumulation. In addition, the cytoplasm showed many vesicles that were different from those accumulating in the growing bud and from the lytic vacuoles of the untreated yeasts. To obtain information about these membranous structures, living cells were observed under phase contrast and UV light microscopes. Furthermore, ultrathin sections of Congo red-treated cells were processed by cytochemical techniques to reveal the chitin areas. Observations suggest that the aberrant vesicles were involved in a secretory process, and that pre-assembled chitin was not among the components transported to the cell periphery.

Chitin↗

[Control of vectors by trapping and its impact on sleeping sickness in a zone of high density of Glossina fuscipes quanzensis (Lefini River, People's Republic of the Congo)].

Tsetse control by trapping and its impact on the transmission of sleeping sickness in the high density area of Glossina fuscipes quanzenis (Léfini river, People's republic of Congo). The focus of human sleeping sickness of the Léfini river is of strategic importance for the People's Republic of Congo as it is located at an important junction which allows the trypanosomiasis to spread over the whole country. A campaign of tsetse control by trapping was undertaken in this focus, where an adjacent game reserve provides a continuous source of flies. The only practicable strategy was the use of a new model of trap with a permanent "killing cage" and which does not need to be impregnated with an insecticide. Transmission of sleeping sickness has been reduced by 1) decreasing the density of flies which are in close contact with man, 2) constantly eliminating flies likely to become infected and transmit the disease.

Animals↗

[Strategy of sleeping sickness control in the Peoples' Republic of Congo: research and perspectives].

The authors review the different strategies applied nowadays in the Popular Republic of Congo to eradicate sleeping sickness. They underline the problems encountered on the field as well as researches carried out to solve them. Recent progress at both levels of screening and trapping opened interesting prospects. Success of the Congo in its fight against sleeping sickness depends now mainly on means made available to apply on a large scale these new methods.

Congo↗

Bilharziasis in Swedish military personnel returning from the Democratic Republic of the Congo.

People from temperate climates often underestimate the danger of contracting bilharziasis in tropical areas. Since 1960, Swedish soldiers have served on UN duty in the Democratic Republic of the Congo and all have been frequently warned of the danger of contact with fresh water. These warnings have sometimes been ignored on the grounds that the likelihood of blood-fluke infection is minimal. The normal health check-up-examination of stools and urine for the presence of eggs and differential count for eosinophilia-had previously led to the diagnosis of only five cases in 5500 men examined. In a more detailed examination of 382 soldiers returning to Sweden from the Congo, 10 of them gave positive reactions to tests for bilharziasis.The value of serological screening has been amply demonstrated, since only one of these cases would have been detected if only conventional methods had been used. If antigens for serological tests are not available, follow-up examination for eosinophilia can provide a valuable aid in the detection of schistosome infections.

Democratic Republic of the Congo↗

Thionine-Feulgen Congo Red -- a new staining technique for automated cytology.

A new staining method has been developed for an automated pre-screening device for cervical smears called BioPEPR. The method is a combination of the Feulgen technique with thionine as th Schiff reagent, and congo red. Thionine and congo red have absorption maxima at 585 nm and 500 nm respectively. The staining results in cells with an orange-red cytoplasm and brownish-blue nucleus. These smears can e easily screened using a normal microscope. The BioPEPR system scans photonegatives of the smears that are made at a magnification of ten using monochromatic light of 545 nm. The staining facilitates good discrimination between background and cytoplasm and between cytoplasm and nucleus, allowing morphologic parameters such as cytoplasmic area, nuclear area and nuclear integrated darkness to be well measured.

Autoanalysis↗

[Role of interhuman infection during the cholera epidemic in the People's Republic of the Congo (1978-1979)].

The authors make an epidemiologic analysis of the first cholera cases notified by the Congo in 1978 and 1979. Contrary to the explosive cholera, experiences by Burundi and Zaïre during the same period, the cholera, in the Congo, had a very subtle propagation, with the well-know "iceberg" phenomena. Human contacts were very important in the transmission of the disease. The cases encountered were almost exclusively of the family type. Healthy carriers represent a threat to the other countries.

Adolescent↗

[Main causes of child blindness in People Republic of Congo (author's transl)].

Report from 18 months of practice in the "Hôpital Général" of Brazzaville. Three groups must be considered: -- Newborns: -- the purulent gonococcal ophtalmia has a bad prognosis and though rather rare, must not be neglected. -- Infants: -- measles, which have in Congo a very high prevalence, induce very frequently corneal ulcers with a great risk of rapide evolution towards hypopyon or panophtalmia; -- congenital cataracts probably related to rubella or toxoplasmosis. -- Children: -- congenital glaucoma; -- retina, with, in first rank, the retinoblastoma which seems particularly frequent in People Republic of Congo; -- traumatisms of various kinds.

Adolescent↗

The detection of specific acute phase serum protein complexes and immune complexes by congo red binding.

The study concerns the problem of selective binding of Congo Red dye to serum proteins. The dye-binding structural motifs were assumed to appear in proteins as the result of local disruptions of tertiary interaction associated with biological activities. Functional structures of serum proteinase inhibitors and haptoglobins induced by interaction of these proteins with their natural substrates were found to bind Congo Red. Soluble immune complexes formed by bivalent hapten-bridged anti-TNP rabbit antibodies also appeared able to interact with the dye. The possible use of these effects for diagnostic analysis was suggested.

Acute-Phase Proteins↗

Is body mass index sensitively related to socio-economic status and to economic adjustment? A case study from the Congo.

Several nutritional surveys based on representative samples from various urban and rural situations show that the Congo presents a situation of nutritional transition. There is a large prevalence of low body mass index (BMI) in adults from rural zones and this increases with age. There is, however, a large prevalence of high BMI in urban populations despite the persistence of some degree of chronic energy deficiency (CED), particularly at younger ages. Correspondence analysis and logistic regression were used to construct a socio-economic index and measure adjusted risk factors for CED. In rural areas, the major risk factors were old age, sex (women) and the absence of schooling; low economic status, a commonly shared factor, did not differentiate between households for CED. In Brazzaville, CED was linked to a young age (< 30 years) and, clearly, to poverty. The change in the prevalence of CED in mothers from the capital city during a period of economic adjustment showed an increased incidence in young mothers, and also showed that the disparity between low and high economic levels regarding CED had grown. Finally, there was a high level of correspondence between the mean values for the weight-for-height of children and the BMI categories of the mothers. There is a parallel evolution during the period of economic adjustment between the increase of wasting in infants and the increase of CED in mothers. Therefore BMI appears to be a potential core indicator for use in nutritional surveillance in the Congo.

Adolescent↗

[Prevalence of toxoplasmosis in Pointe-Noire in Congo. Study of the sampling of 310 subjects].

The prevalence of toxoplasma antibodies was studied in 310 individuals in the city of Pointe-Noire in Congo. The age ranged from 1 to 50 years. The seroprevalence in this study population was 41.9%. Seroprevalence increase with age until 20 years of age, indicating that infection is acquired early in life. Because the seroprevalence did not significantly increase after 20 years of age, we postulate that acquisition of infection is acquired via contact/ingestion of soil contaminated with oocysts rather than via ingestion of undercooked meat containing toxoplasma cysts. No differences were observed in seroprevalence between the sexes and between the different ethnic groups analysed. However, significant differences in the seroprevalence were observed in individuals originated from different regions of the Congo. These high prevalence rates noted in this study also alerts physicians on the risk of reactivation of chronic toxoplasma infection in the immunocompromised patients in particular those coinfected with HIV.

AIDS-Related Opportunistic Infections↗

[Complementary nutrition for the young child following the devaluation of the CFA franc (African Financial Community): 2 case studies in the Congo and Senegal urban environment].

Developing countries frequently see their currency depreciated to varying degrees. The consequences of such monetary disturbances on the nutrition of young children are not well known, though children are the most vulnerable in nutritional terms. One year after the 50% devaluation of the CFA Franc (communauté financière africaine, "African Financial Community"), which took place on 12 January 1994 simultaneously in fourteen countries, nine of which are on the UNDP list of least developed countries, we wanted to find out the long-term effects of the devaluation, and the strategies that families had adopted to cope with it. In Brazzaville, Congo, in December 1994, an epidemiological survey was conducted on a representative sample of 893 children between the ages of 4 and 12 months in two districts, and indicators of child nutrition were established. A comparable survey had been conducted in December 1993, before the devaluation. In Senegal, in the absence of a previous survey which could be used in comparison, a qualitative survey using RAP methodology, was conducted in January 1995 in two towns near the capital. In three districts in each of these towns, a cluster of ten plots was chosen at random and surveyed, with a combination of semi-structured individual interviews with mothers (n = 60) and group interviews with all the women together (n = 6). The information was put together with interviews of 25 local traders selling food. In the Congo, comparison of the two surveys shows that the practice of breast-feeding had hardly changed, nor had the age at which baby food was introduced (90% of children of 4-5 months take semi-solid and solid foods); on the other hand, more children are being given the ordinary family meal earlier, at 6-9 months. The proportion of baby foods based on commercially imported flour has fallen (from 32% in 1993 to 18% in 1994), and has been replaced with local products based on maize; this change is more marked among poorer families. The low nutritional value of such preparations is in part compensated by the addition of sugar, though less milk is added (28% in 1994 as opposed to 43% in 1993). In Senegal, mothers do not seem to have changed their breast-feeding practices either, the age at which baby foods are introduced, or the number of times they are provided daily. The most important change is the drop in quality of food given to children, and the poorer family food for the older children. The partial switch from imported products to local produce was an expected consequence of devaluation; it is clearly confirmed here for nutrition of young children, with the consequent loss of nutritional quality (a reduction in energy density and in nutrients). The first thing needed is, therefore, an improvement in local manufacture of food supplements of good nutritional quality, for young children. Mothers also complain of the increased difficulty in managing a family diet so as to take account of economic needs, cultural values and nutrition. They therefore criticize a number of nutritional education messages that are clearly no longer appropriate to the new economic context. Finally the fact that young children are getting poorer quality nutrition is worrying for the future: if it lasts, the nutritional status of children will deteriorate; whenever possible, monitoring must be established so that measures can be taken when necessary to forestall any dramatic deterioration that would endanger the health of the children.

Adult↗

Chronic atrophic fundic gastritis diagnosed by a modified Congo red test.

BACKGROUND AND STUDY AIMS: Chronic atrophic fundic gastritis (CAFG) is associated with several diseases, such as gastric cancer, gastric ulcer, pernicious anemia, and bacterial overgrowth. In spite of recent technical improvements, the gastroscopic diagnosis of CAFG remains uncertain. Congo red chromogastroscopy is capable of visualizing acid-producing normal fundic mucosa, but has hitherto not been suitable for routine use. The aim of our study was to establish a reliable endoscopic technique with which to diagnose CAFG. PATIENTS AND METHODS: This prospective study comprises 124 consecutive patients (71 women, 53 min) with a mean age of 65 years (range 36-92). Macroscopic evaluation of the gastric fundic mucosa in routine endoscopy using video techniques was compared with evaluation by means of a modified endoscopic Congo red test (MCRT). In routine gastroscopy, CAFG was recognized by the thin, friable mucosa, with a marked visible vascular pattern and fold atrophy. With MCRT, the diagnosis of CAFG was made within five minutes' observation when no red-to-blue color shift in the fundic mucosa could be induced by 0.2 mu g/kg intravenous pentagastrin. The results were then compared with the histological examination of biopsies from the fundic mucosa. RESULTS: CAFG was confirmed by histology in 40 of 124 cases. The diagnostic sensitivity of MCRT was 1.0 (40/40), with a positive predictive value of 0.90, whereas the values for macroscopic gastroscopic evaluation were 0.25 (10/40) and 0.50, respectively. CONCLUSIONS: We conclude that MCRT is a sensitive, fast, and cost-effective method of identifying patients with CAFG, and well suited for use in routine gastroscopy.

Adolescent↗

Rheumatoid arthritis in Congo-Brazzaville. A study of thirty-six cases.

OBJECTIVES: To define the epidemiology, clinical features and outcomes of rheumatoid arthritis in a hospital-based population in Congo-Brazzaville. PATIENTS AND METHODS: We retrospectively reviewed the medical records of the 36 subjects seen on an inpatient or outpatient basis at our rheumatology department who met American College of Rheumatology criteria for rheumatoid arthritis. RESULTS: There were 28 women and eight men (male to female ratio, 0.28), with a mean age of 43.5 years. The total number of patients seen at our department during the study period was 3518, yielding a prevalence of 1% for rheumatoid arthritis. Seven per cent of the patients with inflammatory joint disease had rheumatoid arthritis. Mean disease duration was 9.5 years (range, 1-25 years). The onset was gradual in every case and distal joints were affected first in 33 patients. All the patients but one had bilateral symmetric polyarthritis; affected joints included the proximal interphalangeal joints (n: 33), the metacarpophalangeal joints (n: 27), the wrists (n: 27), the metatarsophalangeal joints (n: 20), the knees (n: 26), the elbows (n: 18), the hips (n: 4), the temporomandibular joints (n: 4), and the cervical spine (n: 5). Boutonnière deformity of the fingers was the most common joint deformity (n: 15), followed by ulnar drift (n: 9). Three patients had tenosynovitis, two had tendon rupture, one had carpal tunnel syndrome and one had rheumatoid nodules. Rheumatoid factors were found in 70% of cases. Evidence of chondrolysis was seen on roentgenograms in every case. Gold and chloroquine were the two most commonly used second-line agents. Seven patients were on prednisone, in a mean daily dose of 6 mg. Mean follow-up was 15 months (range: 3-36 months). Steinbrocker's disease activity stage was IV in 14 patients and III in eight patients; Steinbrocker's functional stage was IV in five patients and III in three. CONCLUSION: The features and outcomes of rheumatoid arthritis in Congo-Brazzaville match textbook descriptions except for a lower prevalence of extraarticular manifestations. Similar findings have been reported for East Africa, South Africa, and some countries of West Africa.

Adult↗