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[Feeding preferences in 3 species of Triatominae (Hemiptera: Reduviidae) in experimental conditions].

Feeding preferences of three species of triatomines (Triatoma infestans, T. dimidiata, and Rhodnius prolixus) on four vertebrate hosts (opposum, dog, chicken and toad) were studied in a specially designed cage during daylight (2 hours) and at night (14 hours). T. infestans and R. prolixus remained close to the host after feeding, while T. dimidiata wandered a short distance away, apparently for protection against preying by the host. R. prolixus showed the lightest values of ingestion in relation to its own weight. The amount of blood ingested by the three species was not related to the type of host. The percentage of insects that fed during the short and long periods of exposure, and the amount of blood ingested were similar in both experiments. The vertebrate hosts showed no prey preference for any of the three species. All the triatomines exhibited a marked preference for the homeothermic hosts, only a very small percentage (0.7%) of T. dimidiata, the least aggressive of the three species, fed on the toad; the insects that fed on two or more hosts did not exceed 8.1% T. infestans showed a slight preference for dogs. During nocturnal experiments all three species preferred to feed on hosts that slept at this time.

Animals↗

[Morphological study of ovum and nymphs of Dipetalogaster maximus (Uhler, 1894) (Hemiptera, Reduviidae, Triatominae)].

Intending to provide new parameters to be used in Triatomines' Taxonomy, vectors of Chaga's Disease and to amplify a large range of knowledge about this species, a study of the structure of the egg and external morphology of the five nymphal instars was done. The eggs show under optical microscope (M.O.), the chorium's surface of the body and the operculum formed by poligonal areas, clearer under scanning electronic microscope (MEV). The apex of the third rostrum segment in all 5 instars nymphs, show 1 + 1 invaginated and elongated structures and the apical portion of the second tarsal segment presents a group of more or less numerous, very long, delicated and golden hairs.

Animals↗

[Triatominae capable of transmitting Trypanosoma cruzi in Cuba].

A brief review of the species of the genus Triatoma that have been described in some epidemiology books is made. Triatoma rubrofasciata has been found at the Oriente province in our country. Triatoma flavida has been found at the Pinar del Río, Las Villas, Camagüey, and Oriente provinces. Both insects transmit the Chagas' disease.

Animals↗

Variability of the salivary proteins of 20 Brazilian populations of Panstrongylus megistus (Hemiptera: Reduviidae: Triatominae).

The objective of the present study was to study variability in the salivary proteins of 20 Panstrongylus megistus populations from different ecotopes and verify whether this variability influenced the intensity of the response to specific anti-saliva antibodies. Electrophoretic analysis of P. megistus saliva showed a complex protein composition and great interpopulation variability. A higher concentration of bands was observed in the 17-29 kDa region. The phenogram constructed from the electrophoretic profiles of the P. megistus study populations revealed the existence of two main groups. However, there was no evident relationship between these groups and geographical regions, ecotopes or hosts. Saliva inoculated by P. megistus during feeding elicited production of low level of anti-saliva antibodies in rabbit. The homologous and heterologous salivary proteins were recognised by serum of rabbit sensitised with saliva from only one population. Qualitative and quantitative differences were observed for recognised bands in the saliva of all eight populations studied by Western blot analysis. The most recognised bands were those of greatest molecular weight (68.0-97.4 kDa).

Animals↗