Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Trephining”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 199 records · Page 11Linked to original sources
PubMed1993

Collagenase digestion of bone marrow trephine biopsy specimens: an important adjunct to haematological diagnosis when marrow aspiration fails.

Failure to obtain sufficient material from marrow aspiration (dry tap) posed a diagnostic problem in two patients with pancytopenia. By using collagenase digestion of the trephine biopsy specimen, a precise diagnosis was reached. This technique is very useful because it permits flow cytometric and immunocytochemical analyses of cell suspensions obtained after collagenase digestion of the trephine biopsy specimen core. Acute leukaemia presenting with a dry tap can therefore be accurately immunophenotyped. The technique is easy to perform and merits wider use.

Adult↗
PubMed1975

Trephine bone biopsy by radiologists: results of 73 procedures.

Trephine bone biopsies were performed in 67 patients. Routine radiography, laminagraphy, and radioisotopic techniques were employed to determine areas of involvement and exact sites for biopsy. Sites were selected on the basis of accessibility, possible complications, and chances of recovering diagnostic material. The biopsy material was informative in 81% of patients. One controllable hemorrhage, one temporary sinus tract, a small pneumothorax requiring no therapy, and two benign vasovagal responses were the only complications. Widespread malignant disease and osteomyelitis, particularly disk-space infection, are the most suitable lesions for trephine biopsy.

Biopsy, Needle↗
PubMed2006

Cranioplasty after trephination using a novel biodegradable burr hole cover: technical case report.

OBJECTIVE AND IMPORTANCE: We have developed novel biodegradable polymer implants by using the rapid prototyping technology fused deposition modeling. Early results of a clinical pilot study for cranioplasty are presented. CLINICAL PRESENTATION: Five patients with the diagnosis of chronic subdural hematoma were included in the study. After trephination and evacuation of the subdural hematoma, burr holes (diameter, 14 mm) were closed using a biodegradable implant made of polycaprolactone. Implants were computer designed with an upper rim diameter of 16 mm and a 14 mm body diameter with a fully interconnected, honeycomb-like architecture of 400 to 600 microm in pore size. INTERVENTION: Postoperative computed tomographic scans indicated that the plugs were stably anchored in the osseous host environment with no fluid collection detectable. The postoperative course was uneventful, and patients were discharged after 5 days. Follow-up scans after 3, 6, and 12 months showed that the implants were well integrated in the surrounding calvarial bone with new bone filling the porous space. CONCLUSION: These novel polymer scaffolds made of the slow-degrading material polycaprolactone represent a suitable implant for closure of post-trephination defects.

Absorbable Implants↗
PubMed1993

The anterior interhemispheric trephine approach to anterior midline aneurysms: results of treatment in 72 consecutive patients.

We report the results of a prospective study of an anterior interhemispheric trephine approach in 72 consecutive patients with aneurysms of the anterior communicating artery (64 cases) and anterior cerebral arteries (eight cases). The salient features of the operative technique include a limited forehead crease incision, low anterior frontal midline trephine (4 cm), unilateral dural opening (2.5 cm), opening up of the anterior interhemispheric fissure and exposure of aneurysm and anterior cerebral vessels by a phased dissection. The best anatomical orientation is retained without compromising the superior sagittal sinus, falx cerebri, left frontal cortical veins, or eloquent areas of the brain. The results (excellent to good recovery or mild to moderate disability in 87.5% of cases) confirm that the technique is safe and well tolerated even in the elderly or fragile patients.

Adult↗
PubMed1981

Gelfoam paste in experimental laminectomy and cranial trephination: hemostasis and bone healing.

The hemostatic properties and effect on osteogenesis of gelatin foam paste and bone wax were compared on surgical bone lesions in experimental animals. Thirty rabbits each received four trephine craniotomies and four lumbar laminectomies. Alternate bone incisions in each animal were treated with either gelatin foam paste or bone wax. Blood loss was measured by absorbing the blood into dry surgical cottonoids weighed before and after use. Bone healing sites of three rabbits were examined histologically to assess the effect of each agent on osteogenesis. The trephination sites of eight rabbits were subjected to fracture force testing at 6 weeks postoperatively to compare the effect ot the two agents on bone healing. No significant difference was found between gelatin foam paste and bone wax in either effectiveness of hemostasis or effect on osteogenesis.

Animals↗
PubMed2002

CD34/QBEND10 immunostaining in the bone marrow trephine biopsy: a study of CD34-positive mononuclear cells and megakaryocytes.

CONTEXT: The immunohistochemical detection of CD34 protein using QBEND10 antibody in bone marrow trephine biopsies was shown recently to be a precise method for quantitation of blasts and a possibly useful approach in diagnosis and classification of myelodysplastic syndrome. OBJECTIVES: To evaluate CD34+ cells in bone marrow biopsies with various diagnoses and to assess how counts obtained using this method correlate with blast counts obtained by traditional morphologic evaluation of bone marrow smears. DESIGN: Bone marrow trephine biopsies from 108 adult patients were evaluated by immunohistochemistry using anti-CD34 antibody (QBEND10). CD34+ mononuclear cells were counted and compared with the blast counts in the bone marrow aspirate smears or imprints. CD34+ mononuclear cell clusters and CD34+ megakaryocytes were also recorded. The type of positivity (membranous vs cytoplasmic) and the percentage of CD34+ megakaryocytes were evaluated because the presence of CD34+ megakaryocytes was recently suggested to be present in myelodysplastic syndrome, but not in myeloproliferative disease or nonneoplastic bone marrow. RESULTS: Six of 24 biopsies with partial involvement by non-Hodgkin lymphoma and 5 of 60 biopsies with reactive changes had 5% to 10% CD34+ mononuclear cells and were associated with lymphocytosis and increased hematogones. The CD34+ mononuclear cell clusters were found only in myelodysplastic syndrome and myeloproliferative disease. The CD34+ megakaryocytes were present in all diagnostic groups. CONCLUSION: The number of CD34+ mononuclear cells was often slightly higher than the number of myeloid blasts in the bone marrow smears, probably due to increased hematogones. The presence and the number of CD34+ megakaryocytes do not appear to have diagnostic value, but this finding should be further investigated in relation to clinical parameters.

Antibodies, Monoclonal↗
PubMedDate not supplied

Trephine arthrodesis of the foot and ankle: indications, operative technique, and long-term follow-up.

We conducted a retrospective analysis of trephine arthrodesis in a total of 22 joints in 15 feet to determine the efficacy of this procedure in the foot and ankle. Twelve of the 22 joints that were fused involved the tarsometatarsal articulations, 3 the naviculocuneiform joint, 6 the subtalar joint, and 1 the ankle. A successful fusion rate of 95% (21/22) was achieved. These results lend evidence that the trephine arthrodesis technique can be used with satisfactory results and confidence in the foot and ankle.

Adult↗
PubMed1984

[Subdural tension pneumocephalus after trephination for chronic subdural hematoma].

Symptomatic tension pneumocephalus developed after an evacuation of chronic subdural hematoma is reported. In this 71 year-old man, a rapid deterioration of consciousness was noted 5 days after a trephination for bilateral chronic subdural hematomas. An evacuation of hematoma was performed under a local anesthesia, and the preoperative and immediate postoperative course was uneventful. Subdural tension pneumocephalus was confirmed by typical CT findings and treated by re-trephination and drainage. The literature on the tension subdural pneumocephalus was reviewed, and the mechanism of its development was briefly discussed.

Aged↗
PubMed1983

Scleral trephination for subchoroidal effusion.

Posterior scleral trephination at the pars plana ciliaris in a dependent site, with normal conjunctiva covering the opening, proved a reliable method for evacuating subchoroidal effusion in cases of flat anterior chamber. The method was also successful in cases occurring after retinal detachment surgery and after retinal detachment surgery and after expulsive hemorrhage. Unlike linear sclerotomy, drainage of the subchoroidal effusion continues for several days after the trephination.

Aged↗
PubMed1988

A suction trephine block for marking donor corneal buttons.

We describe a technique to accurately align the circumferences of the donor button and host cornea during penetrating keratoplasty. The donor cornea is trephined on a block into which four narrow, intersecting, equally spaced radial cuts are made. Suction is applied to the donor cornea through the four cuts, resulting in four radial marks in the donor corneal epithelium. Four radial marks are also made in the host cornea before trephination, using a radial keratotomy marker. When the donor button is placed within the recipient opening, the radial marks are aligned and used as guides for the cardinal sutures. This simple technique allows for matching of the donor and host circumferences, even if there is some collapse of the peripheral host cornea and sclera at surgery, and should minimize astigmatism resulting from donor/host misalignment.

Astigmatism↗
PubMed1996

Three-dimensional reconstruction of benign lymphoid aggregates in bone marrow trephines.

Distinguishing between deposits of low-grade non-Hodgkin's lymphoma and benign lymphoid aggregates in bone marrow trephine sections is a recognized problem in haematopathology. To test the hypothesis that benign lymphoid aggregates do not make contact with a trabecular surface, three-dimensional models were constructed of five serially sectioned bone marrow trephines, containing a total of 19 lymphoid aggregates known to be benign. The computer-generated images showed that benign lymphoid aggregates were located in the central marrow space and did not become paratrabecular. This suggests that a paratrabecular location may help in some cases to distinguish deposits of low-grade non-Hodgkin's lymphoma from benign lymphoid aggregates.

Aged↗
PubMed1992

An improved means of faecal diversion: the trephine stoma.

Twenty-four patients in whom trephine stoma formation was performed over a 4-year period are described. This procedure permits the formation of an end ileostomy or colostomy without laparotomy. It is simple, rapid, safe and allows speedy recovery. It is widely applicable and previous abdominal surgery is no contraindication. The procedure was impossible in two patients who required laparotomy and in a third in whom a loop colostomy was created. Operation time was shorter and postoperative opiate requirements less than when laparotomy was undertaken for stoma formation. Follow-up (median 1 year) of 17 patients confirmed a low incidence of complications (two prolapses, one parastomal hernia) after trephine stoma formation. This procedure is recommended as the preferred method of ileostomy or colostomy formation when laparotomy is not otherwise indicated.

Adult↗
PubMed1987

The use of the anti-factor VIII method on trephine biopsies of the bone marrow for the identification of immature and atypical megakaryocytes in myeloproliferative diseases and allied disorders. A morphometric study.

A morphometric analysis was performed on trephine biopsies of the bone marrow to identify atypical megakaryocyte proliferation following PAS staining and the immunohistological demonstration of factor VIII. This study includes nine patients with a megakaryoblastic crisis in chronic myeloid leukemia (CML), four with acute megakaryoblastic leukemia (AM) and three with myeloid dysplasia later evolving into overt acute leukemia. Comparison and statistical evaluation of the PAS reaction with anti-factor VIII staining reveals that the latter technique not only facilitates the recognition of immature and abnormal megakaryocytes, but leads to a significantly increased count for all megakaryocytic elements in the bone marrow. Thus our retrospective investigation of routinely processed and paraffin-embedded trephine biopsies shows that the diagnosis of a megakaryoblastic crisis in CML as well as AM may be easily established with the aid of the anti-factor VIII method. In all cases of megakaryoblastic proliferation in CML and AM, the appearance of blasts was associated with moderate to pronounced myelofibrosis which could be also determined by morphometry.

Adult↗
PubMed2005

[Non-contact donor cornea trephination with a flying spot excimer laser system].

BACKGROUND: One of the most common complications after mechanical penetrating keratoplasty is the occurrence of irregular astigmatism. We developed a method to prepare donor grafts for transplantation applying excimer laser techniques. The quality of the cut edges and exposure time were evaluated. MATERIALS AND METHODS: The beam of an experimental excimer laser (OPTex, Lambda Physik) was homogenized and guided by an optical system including lenses and scanner mirrors. A special software was used to conduct the laser beam in a circular mode over the surface of the cornea. Corneas from porcine eyes were stabilized in an artificial anterior chamber. Twenty in vitro explants were trephinated applying our method and subsequently investigated by electron microscopy and histology. RESULTS: All experiments provided regular and smooth cut edges. The average exposure time until perforation was 10.5 min (SD 2.7 min), the diameter of the grafts was 8.0 mm. CONCLUSION: The experiments showed the possibility of non-contact trephination and generation of smooth cut edges with a rotating focussed excimer laser beam in a porcine cornea model. A major advantage of the system is the possibility of customized "tailored" grafts.

Angioplasty, Balloon, Laser-Assisted↗
PubMed1998

[Rise in intraocular tension in microkeratome sections (LASIK) and with the GTS trephine system for keratoplasty].

UNLABELLED: Microkeratome systems are being increasingly applied in lamellar corneal surgery (e.g., LASIK). The results of the few studies on intraocular pressure changes during the microkeratome cut vary widely. In this study, we examined the intraocular pressure pattern (IOP) in relation to the initial pressure. METHODS: We performed lamellar corneal cuts on freshly enucleated porcine bulbs with three different microkeratome systems and different setting for the initial pressure (10, 20 and 30 mm Hg). The bulbs were cannulated, and the IOPs were recorded online with a two-chamber measuring system. In addition, we examined the GTS trephine system applied for corneal transplantation. RESULTS: It became obvious that there were significant differences between systems (significance level, P = 0.05). With an initial pressure of 10 mm Hg the measured values of the Schwind system are significantly lower than with the Polytech system, and these values are again significantly lower than with the Chiron microkeratome. With 20 mm Hg the Chiron system shows significantly higher values than both other systems. With 30 mm Hg initial pressure, the pressure values with the Schwind system and with the Chiron microkeratome are significantly higher than with the Polytech system. Thus with all microkeratome systems the middle and maximum pressure values rise in correlation with the initial pressure levels. This effect was not seen with the trephine system. With the same initial pressure, the standard deviation of the measured data of different mikrokeratomes was low (range 3.1-9.0 mm Hg). CONCLUSION: The pressure values shown are higher than the data published by the manufacturers. There is current discussion as to whether these pressure values are dangerous (e.g. bleeding) or not. The pressure values reached intraocularly during the microkeratome cut depend on the system and also on the present initial intraocular pressure values. Only with the same initial pressure can reproducible applanation and cutting conditions be obtained, which guarantee stable quality of the cut.

Animals↗
PubMed2003

Effects of early postoperative complications and the location of trephined block on long-term intraocular pressure control after trabeculectomy.

BACKGROUND: Complications of trabeculectomy in eyes with primary open-angle glaucoma (POAG) and exfoliation glaucoma (EG) were compared. The effects of complications and the location of the trephined tissue block on the long-term success were evaluated. METHODS: One hundred thirty-eight consecutive eyes with POAG or EG of patients over 40 years of age operated on by one surgeon by trabeculectomy without using antimetabolites were analyzed retrospectively. Early postoperative complications and any additional postoperative procedures were recorded and related to the final outcome. In 118 eyes the location of the trephined tissue block was defined in a light microscopic study as anterior or posterior. The success rates in these two groups were determined by Kaplan-Meier survival curves, and the complication rates were compared. RESULTS: Postoperative complications were equally common in POAG and EG, except for hyphema, which was significantly more common in EG than in POAG. Of the early complications, only insufficient filtration at the end of the 1st postoperative month was significantly associated with failure in the long term. The location of trabeculectomy did not significantly affect the early postoperative complications or the long-term success. CONCLUSIONS: Early postoperative complications after trabeculectomy were common, but the only significant predictor of failure was increased intraocular pressure at the end of the 1st postoperative month.

Adult↗
PubMed2005

Ancillary techniques in bone marrow pathology: molecular diagnostics on bone marrow trephine biopsies.

Pathologic examination of trephine bone marrow (BM) biopsies plays a central role in the diagnosis and staging of haematological neoplasms and other disorders affecting haematopoiesis. Haematopathology has been profoundly influenced by the advent of molecular genetic techniques suitable for paraffin-embedded tissues, and certain applications, such as the determination of B- and T-cell clonality, belong to its standard diagnostic repertoire. Many of these molecular tests can be performed successfully with nucleic acids extracted from BM trephine biopsies, if some technical aspects specific to this template source such as various fixation and decalcification procedures are taken into consideration. The current indications for molecular BM diagnostics range from the confirmation of lymphoma involvement with gene rearrangement analysis, demonstration of tumor-specific translocations in lymphoid and chronic myeloproliferative disorders along to the detection of microorganisms or marrow involvement by soft tissue sarcomas. The availability of quantitative polymerase chain reaction techniques for the investigation of allelic imbalances and gene expression levels in paraffin-embedded material also open new avenues for research and advanced diagnostics. The molecular detection of minimal residual disease in haematological neoplasms, especially in the context of new treatment strategies, will provide future challenges. This article summarizes the current state of the art in molecular diagnostics applied to paraffin-embedded BM biopsies.

Biopsy↗