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Pollen-stigma interaction in Brassica oleracea: the role of stigmatic proteins in pollen grain adhesion.

The adhesion of pollen grains to the stigmas of Brassica oleracea was assayed after treatment of the stigmas wiuth protease and/or cycloheximide. Treatment with protease alone adversely affected pollen grain adhesion. However, the adhesive properties of the stigma recovered fully if the stigmas were not pollinated until 2 h after treatment. Immersion of the stigmas in cycloheximide after protease treatment prevented any recoveryt of the stigmas' adhesive properties. Cycloheximide treatment alone prevented pollen grain adhesion when pollination occurred later than 1--2 h after treatment but did not affect pollen grain adhesion if pollination occurred immediately after treatment. These results indicated not only that the surface-held proteins of the stigma are involved in pollen grain adhesion, but also that their turnover rate is rapid. Isoelectric focusing of extracts derived from stigmas after protease and cycloheximide treatment showed a marked decrease in staining intensity of 3 protein bands, one of which, a glycoprotein, is known to be present only when the self-incompativility system is fully functional. These observations suggest a specificity of adhesion between higher plant cells in the presence of the cell wall.

Brassica↗

Stigmatizing a "normal" condition: urinary incontinence in late life.

The geriatric medical literature presents a perspective on urinary incontinence in the elderly that is sharply divergent from the realities of medical and lay responses to incontinence. This contrast raises questions about the cultural significance of urinary incontinence. The geriatric literature reveals a consensus that urinary incontinence, a major health problem among the elderly, is treatable and frequently reversible. The elderly and their health care providers, however, not only see incontinence as an inevitable, irreversible, and normal part of growing old but also consider it a sign of incompetence. This linkage of incontinence with incompetence forces elderly people to adopt several strategies for managing their incontinence so as not to compromise their competence in the eyes of others. Incontinence is a cultural symbol for the increasing dependencies of old age, dependencies that are much feared and resented in U.S. society, where tremendous emphasis is placed on independence even into advanced old age.

Activities of Daily Living↗

Stigmatization of AIDS patients by physicians.

A randomly selected sample of physicians in three large cities was asked to read one of four vignettes describing a patient. They then completed a set of objective attitude measures eliciting their reactions to the patient described in the vignette. The vignettes were identical except that the patient's illness was identified as either acquired immunodeficiency syndrome (AIDS) or leukemia and the patient's sexual preference as either heterosexual or homosexual. Harsh attitude judgements were associated with the AIDS portrayals, as well as much less willingness to interact even in routine conversation when the patient's illness was identified as AIDS. Increasing numbers of AIDS patients will be seeking medical attention from physicians in all areas of the country and it will be important for health care professions to develop programs which counter unreasonable stigma and prejudicial attitudes that may be associated with this illness.

Acquired Immunodeficiency Syndrome↗

Body stereotyping and stigmatization of obese persons by first graders.

14 female and 15 male racially diverse first graders were individually interviewed after being shown a set of same-gender body silhouettes. More children were significantly less likely to want to befriend an endomorphic type. Although more children labeled the ectomorph the "good child," the difference did not reach significance.

Body Image↗