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[Study on tissue culture of Poligonum multiflorum].

In the present thesis, suitable medium for callus inducement and relationship between culture time and callus growth of Poligonum multiflorum were studied by mono-factorial and orthogonal design test. The experiments showed that the best medium for callus inducement was MS + 2,4-D 1 mg/L + 6-BA 1 mg/L + IBA 0.5 mg/L, dark culture was better than light culture, callus grew fast when it was cultured 12 days, whole plant could be induced by callus, the induce rate was 95%.

Culture Media↗

[Cultivation of Polygonum multiforum].

The experiments of some cultivated medthods of Polygonum multiforum show that the direct sowing of seeds is better than the cuttage, the index of reproduction and yield are higher, and the cultivated method can decrease expense. To cultivation period of 5 years, high yield and excellent qualty is available.

Plant Stems↗

[Study on supercritical CO2 extraction of lecithin from radix Polygoni Multiflori Preparata by orthogonal design].

OBJECTIVE: To find out the optimal supercritical CO2 fluid extraction process for Radix Polygoni Multiflori Preparata, the extraction process was studied by orthogonal design with the yield of lectithin as index. METHODS: Four factors were chosen in this experiment, including extractor pressure, extractor temperature, separator I pressure and separator I temperature. The ultraviolet visible spectrophotometry was applied for the determination of lecithin in all different experimental conditions. RESULTS: The optimal supercritical CO2 fluid extraction process was: extractor pressure 32 MPa, extractor temperature 50 degrees C, separator I pressure 6 MPa and separator I temperature 55 degrees C. CONCLUSION: Compared with the traditional extraction methods, SFE-CO2 process has the predominance of saving solvent and shortening the technology process, which is exactly suitable for industralization manufacture.

Analysis of Variance↗

[Systemic contact dermatitis].

The field of systemic contact dermatitis is particularly interesting for an allergist. To think of an allergen with cross-reaction and monosensilization should be the first reflex when an enquiry into a skin problem involves an allergic symptom. The three examples quoted involve Peruvian balm as the sensitizing agent. The causal triggering medicines are all vegetal extracts.

Aged↗