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GIBBERELLIN PRODUCTION IN PEA SEEDS DEVELOPING IN EXCISED PODS: EFFECT OF GROWTH RETARDANT AMO-1618.

The gibberellin content of pea seeds developing in excised pea pods cultured on a medium containing the plant growth retardant AMO-1618 was reduced in comparison with pea seeds cultured on retardant-free medium. The reduction increased with increasing concentrations of AMO-1618. However, at the lowest concentration tested (5 milligrams per liter) the growth of the seeds was not affected whereas their gibberellin content was significantly reduced. In conjunction with earlier work on the effect of growth retardants on gibberellin biosynthesis in the fungus Fusarium moniliforme, these results indicate that AMO-1618 inhibits the biosynthesis of gibberellins in tissues of higher plants in much the same manner it does in Fusarium.

Gibberellins↗

New anthocyanins from purple pods of pea (Pisum spp.).

Two new anthocyanins were isolated from purple pods of pea (Pisum spp.). Their structures were identified as delphinidin 3-xylosylgalactoside-5-acetylglucoside and its deacetylated derivative by the usual chemical degradation methods and by spectroscopic methods such as UV-VIS, MS and NMR. Both pigments showed moderate stability and antioxidative activity in a neutral aqueous solution.

Anthocyanins↗

The causal agents of witches' broom and frosty pod rot of cacao (chocolate, Theobroma cacao) form a new lineage of Marasmiaceae.

The two most devastating diseases of cacao (Theobroma cacao)--the source of chocolate--in tropical America are caused by the fungi Crinipellis perniciosa (witches' broom disease) and Moniliophthora roreri (frosty pod rot or moniliasis disease). Despite the agricultural, socio-economic and environmental impact of these fungi, most aspects of their life cycles are unknown, and the phylogenetic relationships of M. roreri have yet to be conclusively established. In this paper, extensive phylogenetic analyses of five nuclear gene regions (28S rDNA, 18S rDNA, ITS, RPB1, and EF1-alpha) confirm that C. perniciosa and M. roreri are sister taxa that belong in the Marasmiaceae (euagarics). Furthermore, these taxa form part of a separate and distinct lineage within the family. This lineage includes the biotrophic fungi Moniliophthora perniciosa comb. nov. and M. roreri, as well as one undescribed endophytic species. The sister genera to Moniliophthora are Marasmius, Crinipellis and Chaetocalathus, which consist mainly of saprotrophic litter fungi.

Agaricales↗

A field trial of detection and treatment of nerve function impairment in leprosy--report from national POD pilot project.

As part of a collaborative project between the Ministry of Health of China (MOH) and The Leprosy Mission International (TLMI) on leprosy rehabilitation and prevention of disability (POD), a total of 1407 patients was monitored for possible nerve function impairment (NFI) through standardized clinical nerve function assessment between May 1995 and February 1998. Of these, 191 patients were found to have NFI and were put on a fixed regimen of prednisolone. In this study, 36.7% of NFI occurred before diagnosis of leprosy, 35.6% developed during MDT and 25.7% after their release from MDT. Overall, 7.5% (105 out of 1407) of all patients, or 55.9% of patients with NFI, suffered from silent neuropathy. Of the affected nerves, 62.6% had silent neuropathy. Sensory impairment responded to prednisolone satisfactorily, giving a recovery rate of 73.8%, 76.5% and 81.0% in ulnar, median and posterior tibial nerve, respectively. Sensibility in patients even with a NFI duration longer than 6 months made significant improvement (p < 0.05). Motor function improvement was less satisfactory, especially in ulnar and c. popliteal nerve. The possible reasons are analysed. Our findings with regard to sensibility changes confirm that once it becomes clinically detectable, NFI is no longer at the 'early' stage. More sensitive tests are necessary to detect real 'early' sensory impairment in the field. Our study also indicates that with well-trained field staff and proper equipment for nerve function assessment, early detection and treatment of NFI can be practical and effective.

Adolescent↗

Cattle intoxication from Enterolobium contortisiliquum pods.

Three outbreaks of hepatogenous photosensitization caused by pods of Enterolobium contortisiliquum are reported in cattle. Clinical signs were anorexia, depression, photosensitization and abortion. Most affected cattle recovered in 30-40 d. At necropsies, liver was present, the gallbladder was enlarged and edematous, and numerous seeds of E contortisiliquum were in the forestomachs and abomasum. Fruits of the plants administrated to 2 calves produced clinical signs and 2/4 died. Clinical chemistry, gross necropsies and histopathology confirmed gastrointestinal irritation and liver degeneration. One calf dosed with only E contortisiliquum leaves did not develop clinical signs.

Animals↗

Body composition in Mexican adults by air displacement plethysmography (ADP) with the BOD-POD and deuterium oxide dilution using infrared spectroscopy (IRS-DOD).

Thirty four subjects (13 men and 21 women), 24 to 70 years old from northern Mexico, were measured for body density by air displacement plethysmography (ADP) with the BOD-POD, and for total body water by deuterium oxide dilution and infrared spectroscopy (IRS-DOD). Subjects were given a 30 g dose of deuterium oxide. Saliva samples were filtered, sublimated, and deuterium was measured using a Miran 1 FF, IRS. Linear regression of the fat mass (FM) derived from both methods showed that the intercept (0.071) was not different from zero (p = .96) and the slope was 0.96 (p < .0001) demonstrating the techniques to be equivalent. Further, mean FM was 26.7 +/- 12.4 and 25.6 +/- 12.4 kg, for IRS-DOD and ADP techniques, respectively (p = .08). Precision analysis by the model R2 showed that 92.3% of the variability was explained (SEM = 3.4 kg). Bland-Altman analysis showed no significant bias (r = 0.017; p = .93). Mean difference between methods was -1.08 (CI: -2.3 to + 0.13) kg FM.

Adipose Tissue↗

Evaluation of the BOD POD for estimating percent fat in female college athletes.

The purpose of this investigation was to examine the accuracy of percent body fat (%BF) estimates obtained by air displacement plethysmography (ADP) using the BOD POD Body Composition System compared with hydrostatic weighing (HW) in a group of female college athletes (n = 80). In addition, %BF estimates by skinfold measures (SF) were also obtained for comparison. A lean subset (n = 39) of the sample was also examined. Mean %BF estimated for the entire sample by ADP (21.2 +/- 5.9%) was significantly greater than that determined by HW (19.4 +/- 6.4%) and SF (18.8 +/- 5.5%). Results from the lean subset also revealed that %BF determined by ADP (17.1 +/- 3.7%) was significantly higher than %BF estimates by HW (14.3 +/- 2.8%) and SF (15.2 +/- 3.2%). The regression equation for the entire sample (%BF HW = 0.937%BF ADP - 0.452, r(2) = 0.73, standard error of estimates (SEE) = 3.34) did not differ from the line of identity. In contrast, the line of identity differed significantly from the regression equation for the lean subset of female athletes (%BF HW = 0.48%BF ADP + 6.115, r(2) = 0.41, SEE = 2.18). The results of this investigation indicate that ADP significantly overestimated %BF by 8% in female athletes and by 16% for a leaner subset of the sample compared with HW. It appears that %BF estimates by SF may be more accurate than those obtained by ADP for female college athletes, regardless of body composition. Coaches and trainers evaluating body composition should consider the use of SF before ADP when measuring %BF in female college athletes. Sports scientists should continue to examine the possible gender and body composition bias for ADP.

Adult↗

Occurrence and cross infectivity of granulovirus of field bean pod borer Adisura atkinsoni Moore.

A granulovirus (GV) was isolated from the field-bean pod borer, Adisura atkinsoni. Electron microscopic observation showed capsule or granular shaped occlusion bodies. The virus was highly virulent against second instar larvae when tested at 1 x 10(6) occlusions/larva through food surface (pod/seed) contamination technique. The incubation period ranged from 6-10 days in the case of second instar larvae. In contrast to green coloured healthy larvae. GV infected A. atkinsoni became brownish/pale white in colour mostly due to accumulation of large number of occlusion bodies. Study on the cross infectivity of A. atkinsoni GV to gram caterpillar, Helicoverpa armigera revealed the high susceptibility of H. armigera to A. atkinsoni GV, thereby widening the scope of controlling both the species on the same cropping system. This is the first record of GV from A. atkinsoni from India.

Animals↗

The molluscicidal activity of seed pods of Swartzia madagascariensis on Marisa cornuarietis.

The molluscicidal potency of ground seed pods of Swartzia madagascariensis was tested in the laboratory against Marisa cornuarietis, a well known competitor and/or predator of pulmonate intermediate hosts of schistosomiasis. Results showed that a minimum concentration of 1.6g ground pods/1 was required to cause 100% mortality in M. cornuarietis. This concentration was by far over and above what is required (ie, 100 mg ground pods/1) to kill 10% of the pulmonate hosts of schistosomiasis. It seems that the application of S. madagascariensis at a concentration inimical to pulmonates (ie, 100 mg ground ponds/1 or slightly higher) in habitats where M. cornuarietis is introduced may not adversely affected to competitor snail population. Hence, the prospect of using S. madagascariensis in conjunction with M. cornuarietis against pulmonate snail hosts is conceived and tentatively recommended.

Animals↗

[Clinical study of a new preparation from plantago seeds and senna pods].

The lack of fiber in the western diet may contribute to the development of several diseases including gastrointestinal disorders; the clinical effects of a new substance (AGIOLAX) made from plantago seeds and senna pods were studied. 100 patients of both sexes, aged from 40 to 60 years (30 with diabetes mellitus, 40 with obesity and 30 with hyperlipidemia) were treated; everyone complained a slowness, of different degree, of normal intestinal transit time or chronic constipation. The experiment was carried out without the use of a control group. Aim of the present study was to investigate the efficacy and tolerability of the product. In addition to the clinical evaluation of the symptoms, laboratory tests were performed. The patients were treated for 3 months with a daily dose of 2 teaspoons every evening. In the majority of the subjects a good clinical response was obtained; 88% of the patients presented a normalization of the gastrointestinal transit time; only 12% of them did not respond satisfactorily to the substance. Further the drug was well tolerated by 86% of the patients. In conclusion the authors report a good efficacy and tolerability of the product; thus they recommend its use in those disorders characterized by slow intestinal transit time and/or constipation.

Adult↗

Hydra and Niccolo Paganini (1782-1840)--two peas in a pod? The molecular basis of extracellular matrix structure in the invertebrate, Hydra.

The body wall of Hydra is organized as an epithelial bilayer with an intervening extracellular matrix (ECM). Molecular and biochemical analyses of Hydra ECM have established that it contains components similar to those seen in more complicated vertebrates such as human. In terms of biophysical parameters, Hydra ECM is highly flexible; a property that facilitates continuous movements along the organism's longitudinal and radial axis. A more rigid ECM, as in vertebrates, would not be compatible with this degree of movement. The flexible nature of Hydra ECM can now be explained in part by the unique structure of the organism's collagens. Interestingly, some aspects of the structural features of Hydra collagens mimic what is seen in Ehlers-Danlos syndrome, an inherited condition in humans that results in an abnormally flexible ECM that can be debilitating in extreme cases. This review will focus on structure-function relationships of the ECM of Hydra.

Animals↗

An inhibitor of nuclear export activates the p53 response and induces the localization of HDM2 and p53 to U1A-positive nuclear bodies associated with the PODs.

Leptomycin B is a cytotoxin which directly interacts with and inhibits the action of CRM1, an essential mediator of the nuclear exit of proteins containing nuclear export signals (NES) of the HIV1 REV type. We show that addition of leptomycin B to human primary fibroblasts increased the levels of the p53 tumor suppressor protein. This was accompanied by the induction of p53-dependent transcriptional activity in cultured cells and an increase in the levels of the products of two p53-responsive genes, the p21(CIP1/WAF1) and HDM2 proteins. Leptomycin B induced the accumulation of p53 and HDM2 in the nucleus and the appearance of discrete nuclear aggregates containing both proteins. It has been reported that the transcriptional activity of p53 is modulated by its interaction with the HDM2 protein which also targets p53 for rapid degradation. Using a model cell line conditionally expressing MDM2, the murine analogue of HDM2, we present evidence indicating that leptomycin B abrogates MDM2's role in p53 degradation and that the accumulation of p53 in distinct nuclear bodies is mediated by MDM2. Since HDM2 has recently been shown to contain a functional NES of the REV type, the most likely explanation for our results is that the effect of leptomycin B on HDM2 and p53 is due to the inhibition of nuclear export. The ability to visualize sites where p53 and HDM2 colocalize provides a new approach to study the association between the two proteins in vivo. These p53/HDM2-positive nuclear foci were found to also contain the U1A snRNP A and to be juxtaposed to the PML oncogenic domains.

Biological Transport↗