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The use of hydrogenated fish oil to extend vegetable oil.

During the last four years, national production of fish oil has increased appreciably due to the expansion of the "reduction" industries (fish flour). Nevertheless, this production is almost totally destined to industrial use (lubricants, tannery, etc.). In contrast, some countries like Japan, Canada and Peru have been using oil from different fish species for human consumption. In view of the above, the objectives of the present work were: first, to establish the experimental conditions for obtention of a hydrogenated fish oil and second, the formulation of fish and vegetable oil mixtures for use in the food industry. The methodology followed comprised: 1) characterization of crude fish oil through physical and chemical analysis; 2) adaptation of the vegetable oil refining procedures to fish oil, and 3) development of fish oil with vegetable oils. A hydrogenated fish oil with a melting point of 36 degrees C, iodine index of 80, and lovibond color, yellow 20, red 13, was produced. Based on the results of the sensory test, the possibility of using up to 30% extension of this oil with vegetable oils for frying, and up to 50% extension for baking, without affecting acceptability of product, was established.

Fish Oils↗

Activities of liver microsomal fatty acid desaturases in zinc-deficient rats force-fed diets with a coconut oil/safflower oil mixture of linseed oil.

The present study was conducted to investigate the effect of zinc deficiency on fatty acid desaturation in rats fed two different types of dietary fat, a mixture of coconut oil and safflower oil (7:1, w/w, "coconut oil diet") or linseed oil ("linseed oil diet"). In order to ensure an adequate food intake, all rats were force-fed by gastric tube. Zinc deficiency caused statistical significant reduction of delta 9-desaturase activity in liver microsomes of rats fed coconut oil diet and tendencial reduction (p < 0.15) in rats fed linseed oil diet compared with control rats fed diets with the same type of fat. In agreement with this effect, zinc deficiency in the rats fed both types of dietary fat increased the ratio between total saturated and total monounsaturated fatty in liver phospholipids and liver microsomes. Zinc deficient rats on the coconut oil diet had unchanged delta 6-desaturase activity with linoleic acid as substrate and lowered activity with alpha-linolenic acid as substrate. In contrast, zinc deficient rats on the linseed oil diet had increased delta 6-desaturase activity with linoleic acid as substrate and unchanged activity with alpha-linolenic acid. Because linoleic acid is the main substrate for delta 6-desaturase in the rats fed coconut oil diet, and alpha-linolenic acid is the main substrate in the rats fed linseed oil diet, it is concluded that in vivo delta 6-desaturation was not changed by zinc deficiency in the rats fed both types of dietary fat. Activity of delta 5-desaturase was also not changed by zinc deficiency in the rats fed both dietary fats. Levels of fatty acids in liver phospholipids and microsomes derived by delta 4-, delta 5-, and delta 6-desaturation were not consistently changed by zinc deficiency in the rats fed both types of dietary fat. Thus, the enzyme studies and also fatty acid composition data of liver phospholipids and microsomes indicate that zinc deficiency does not considerably disturb desaturation of linoleic and alpha-linolenic acid. Therefore, it is suggested that similarities between deficiencies of zinc and essential fatty acids described in literature are not due to disturbed desaturation of linoleic acid in zinc deficiency. The present study also indicates that zinc deficiency enhances incorporation of eicosapentaenoic acid into phosphatidylcholine of rats fed diets with large amounts of n-3 polyunsaturated fatty acids.

Alkaline Phosphatase↗

Are olive oil diets antithrombotic? Diets enriched with olive, rapeseed, or sunflower oil affect postprandial factor VII differently.

BACKGROUND: The incidence of ischemic heart disease (IHD) in Crete was lower than expected on the basis of blood lipid concentrations of participants in the Seven Countries Study. A favorable effect of a high intake of olive oil on thrombogenesis may have contributed to this finding. OBJECTIVE: We compared the effects of virgin olive oil with those of rapeseed and sunflower oils on blood coagulation factor VII (FVII), a key factor in thrombogenesis. DESIGN: In a randomized and strictly controlled crossover study, 18 healthy young men consumed diets enriched with 5 g/MJ (19% of total energy) olive oil, sunflower oil, or rapeseed oil for periods of 3 wk. On the final day of each period, participants consumed standardized high-fat meals (42% of energy as fat). Fasting and nonfasting blood samples were collected after each period. RESULTS: Mean (+/-SEM) nonfasting peak concentrations of activated FVII (FVIIa) were 11.3 +/- 5.1 U/L lower after olive oil than after sunflower oil, an 18% reduction (P < 0.05). Olive oil also tended to cause lower FVIIa peak concentrations than did rapeseed oil (mean difference: 8.6 U/L, a 15% reduction; P = 0.09). There were no significant differences between diets with respect to nonfasting factor VII coagulant activity (FVII:c), prothrombin fragment 1+2 (F1+2), and tissue factor pathway inhibitor (TFPI) concentrations, or with respect to fasting plasma values of FVII protein, FVII:c, FVIIa, F1+2, or TFPI. CONCLUSION: A background diet rich in olive oil may attenuate the acute procoagulant effects of fatty meals, which might contribute to the low incidence of IHD in Mediterranean areas.

Adult↗

Fat infiltration in liver of rats induced by different dietary plant oils: high oleic-, medium oleic- and high petroselinic acid-oils.

Beneficial effects on health parameters by ingestion of plant oils having high levels of monounsaturated fatty acids such as oleic (cis-9-octadecenoic) acid have been described in several studies. Recently, we have shown that feeding of coriander oil containing high proportions of a positional isomer of oleic acid, i.e. petroselinic (cis-6-octadecenoic) acid, led to significantly decreasing proportions of arachidonic (all cis-5,8,11,14-eicosatetraenoic) acid in the cellular lipids. Here, we report histopathological findings in male Wistar rats fed isocaloric diets containing 2% corn oil and 12% (w/w), each, of different plant oils (high-oleic and conventional sunflower oil, olive oil, rapeseed oil as well as coriander oil) containing varying levels of monounsaturated fatty acids, i.e. oleic or petroselinic acids. One group of animals was fed a standard diet containing 4% (w/w) of fat as control. Liver, heart, aorta, stomach, and spleen were anatomically and histologically examined. Livers of animals from all the groups that had received high-fat diets-except the coriander oil group-showed moderate non-degenerative fat infiltrations of the hepatocytes beginning in the periportal fields. Marked to severe fat infiltration was observed in hepatocytes of animals fed coriander oil. In this experimental group the livers were found to contain fatty cysts in addition to hepatocytes with mixed-size lipid vesicles. Moreover, enlarged nuclei were observed in numerous hepatocytes without fat infiltration. Fat infiltration was not observed in livers of animals fed a rat standard diet. In the cells of all other organs and tissues studied, in particular heart and aorta, neither non-degenerative fat infiltrations nor other degenerative changes were observed for any group. Our results show that feeding of fat-rich diets containing varying proportions of C18 cis-monoenoic fatty acids induced different histopathological alterations in the livers of rats, depending on position of the double bond. Plant oils containing varying proportions of oleic acid lead exclusively to moderate fat infiltration in the hepatocytes, whereas in the animals fed high-petroselinic coriander oil degenerative alterations (fatty cysts) as well as -in several cases-enlarged nuclei were found that are likely due to a non-specific stimulation of the hepatocytes resulting from the cell degeneration observed.

Animals↗

Comparative study of the genotoxic properties of Eastern and Western U.S. shale oils, crude petroleum, and coal-derived oil.

A multiple bioassay approach was used to characterize and compare the genotoxicities of an Eastern U.S. (Kentucky) shale oil with the genotoxicities of Western U.S. shale oil, petroleum crude oil, and a coal-derived fuel oil. While the coal-derived oil was mutagenic in the Salmonella/microsome mutagenicity (Ames) assay, the shale oils had negligible to weak mutagenicity, and petroleum crude oil was not mutagenic. All the samples were also tested in the following mammalian test systems: an in vitro sister-chromatid exchange (SCE) assay in human lymphocytes and in vivo tests for induction of sperm abnormalities, micronuclei, and SCE in bone marrow of mice. Slight but statistically significant increases (P less than 0.001) in SCE in human lymphocytes were induced by all samples except petroleum crude oil. Neither sample induced a significant number of mutational events in either of the in vivo systems. In these preliminary studies no major differences in the genotoxicities of Eastern and Western shale oils were observed. The results were consistent with the following order of mutagenic potency: coal oil greater than Eastern and Western shale oil greater than petroleum crude oil.

Animals↗

Effect of plant sterols from rice bran oil and triterpene alcohols from sheanut oil on serum lipoprotein concentrations in humans.

BACKGROUND: Intake of unsaponifiable compounds from edible oils, such as plant sterols, can lower serum cholesterol concentrations in humans. However, little is known about effects of other chemically related unsaponifiables in edible oils, such as triterpene alcohols. OBJECTIVE: We studied the effects of plant sterols from rice bran oil and triterpene alcohols from sheanut oil on cholesterol concentrations in healthy, normolipemic volunteers. DESIGN: Twenty-eight men and 32 women consumed 29 g/d of 3 margarines for 3 wk each on a crossover, double-blind basis. A margarine based on sunflower oil was used as the control. Concentrates of plant sterols from rice bran oil or triterpene alcohols from sheanut oil were added to make 2 experimental margarines with the same fatty acid composition as the control margarine. RESULTS: Intake of 2.1 g plant sterols/d from rice bran oil decreased total cholesterol by 0.19 mmol/L (95% CI: -0.31, -0.07 mmol/L) and LDL cholesterol by 0.20 mmol/L (95% CI: -0.30, -0.10 mmol/L). HDL-cholesterol and triacylglycerol concentrations did not change significantly. Intake of 2.6 g triterpene alcohols/d from sheanut oil did not significantly affect lipoprotein concentrations in all subjects combined. CONCLUSIONS: We found that 2.1 g plant sterols/d from rice bran oil lowered serum total cholesterol by 5% and LDL cholesterol by 9% in normolipemic humans, whereas triterpene alcohols from sheanut oil did not significantly affect lipoprotein concentrations in all subjects combined. The effect of rice bran oil sterols is probably due to ss-sitosterol and other 4-desmethylsterols and not to 4,4'-dimethylsterols.

Adolescent↗

A new method for seed oil body purification and examination of oil body integrity following germination.

Plant seeds store triacylglycerols as energy sources for germination and postgerminative growth of seedlings. The triacylglycerols are preserved in small, discrete, intracellular organelles called oil bodies. A new method was developed to purify seed oil bodies. The method included extraction, flotation by centrifugation, detergent washing, ionic elution, treatment with a chaotropic agent, and integrity testing by use of hexane. These processes subsequently removed non-specifically associated or trapped proteins within the oil bodies. Oil bodies purified by this method maintained their integrity and displayed electrostatic repulsion and steric hindrance on their surface. Compared with the previous procedure, this method allowed higher purification of oil bodies, as demonstrated by SDS-PAGE using five species of oilseeds. Oil bodies purified from sesame were further analyzed by two-dimensional gel electrophoresis and revealed two potential oleosin isoforms. The integrity of oil bodies in germinating sesame seedlings was examined by hexane extraction. Our results indicated that consumption of triacylglycerols reduced gradually the total amount of oil bodies in seedlings, whereas no alteration was observed in the integrity of remaining oil bodies. This observation implies that oil bodies in germinating seeds are not degraded simultaneously. It is suggested that glyoxisomes, with the assistance of mitochondria, fuse and digest oil bodies one at a time, while the remaining oil bodies are preserved intact during the whole period of germination.

Biochemistry↗

High-performance liquid chromatographic determination of vitamin D3 in fish liver oils and eel body oils.

Identification and determination of vitamin D3 (or D2) and 25-OH-D3 in fish liver oils and eel body oils were carried out. By co-chromatography on HPLC, UV spectra and/or GC-MS, vitamin D3 was identified in naturally occurring fish liver oils and eel body oils, whereas a drop of fish liver oil contained supplemented vitamin D2. 25-OH-D3 was identified only in skipjack liver oil. The HPLC method proposed in a previous report (Takeuchi, A. et al. (1984): J. Nutr. Sci. Vitaminol., 30, 11-25) was confirmed to also be useful for determination of vitamin D3 (or D2) in fish liver oils and eel body oils. The assayed values of vitamin D3 in skipjack and tuna liver oils were 57,760 and 16,200 IU/g, respectively, which were much higher than those in cod and pollack liver oils. The assayed values of vitamin D3 in eel body oils were very low (16-43 IU/g) and showed no appreciable change despite differences in the farming conditions. Determination of 25-OH-D3 in skipjack oil was performed by using HPLC, and the assayed value was 1.8 micrograms/g. This was about 1/800 lower than that of vitamin D3.

Animals↗

[Histopathological and ultrastructural characteristics of oil-associated complications in silicone oil-filled human eyes].

OBJECTIVE: To observe morphological changes of oil-associated complications in silicone oil-filled human eyes, and to further explore their pathogenesis. METHODS: The morphology analysis and immunohistochemistrical study were performed in 25 specimens including 8 eyeballs, 1 ocular content, 4 preretinal membranes, 4 corneal buttons and 8 lens from human eyes with silicone oil tamponade. Two of preretinal membranes acquired freshly were also evaluated by transmission electron microscopy (TEM). RESULTS: Endothelium cell loss (90%) and band keratopathy (83%) were the most typical changes in silicone oil-associated keratopathy;while epithelial cell fibrosis was the most frequent histopathological features in silicone oil-associated cataract. In 8 eyeballs and 1 ocular content, it was found that damages to normal retinal layers and formation of preretinal or subretinal membrane with extensive silicone bubbles were obvious in the cases of silicone oil-associated retinopathy, which included loss and degeneration of neuron cells. Moreover, in 3 eyeballs with silicone oil for more than 60 months, retinas were completely replaced by fibril membranes, and the oil vacuoles were also found in sclerocorneal scar, trabecula, iris, ciliary body, choroid, optic nerve and its tunica vaginalis. These finding demonstrated that the longer the silicone oil was retained in eyeballs, the more severe its complications were. Different sizes of silicone bubbles in 2 preretinal membranes were noted easier by TEM than light microscopy. There were some macrophages marker (CD68) positive staining cells in the tissues filled with silicone bubbles, such as preretinal or subretinal membrane and optic nerve. Partial of the membranes surrounding the oil bubbles was positive for GFAP staining, and other part was positive stained for Vimentin. CONCLUSIONS: Intraocular silicone oil can damage the normal tissue structures and function if it is retained in eyeballs too long. This results suggest that silicone oil should be removed timely after the retinal reattachment stabilized and can not be used as a kind of long term intraocular tamponade.

Adolescent↗

The n-3 polyunsaturated fatty acid levels in rat tissue lipids increase in response to dietary olive oil relative to sunflower oil.

In the present study, changes in phospholipid compositions of liver microsomes, erythrocyte membranes, platelets, aorta, cardiac muscle and brain of rats fed olive oil were compared with those of rats fed sunflower oil. Four groups of rats starting at weaning were fed for four weeks a basal diet containing 5 or 25% olive oil or sunflower oil. We found that oleic acid was higher and linoleic acid was lower in membrane phospholipids of olive oil fed rats compared to sunflower oil fed rats. Polyunsaturated fatty acids of the n-3 series were markedly elevated in all tissues of rats on the olive oil diets relative to those on the sunflower oil diets. The results are consistent with a lower linoleic/linolenic acid ratio induced by the olive oil diets, suggesting a positive correlation between olive oil ingestion and n-3 polyunsaturated fatty acid levels in cell and tissue lipids. The study suggests that an adequate intake of olive oil may enhance the conversion of n-3 fatty acids.

Animals↗

Synchronous fluorescence spectroscopy for quantitative determination of virgin olive oil adulteration with sunflower oil.

Adulteration of extra virgin olive oil with sunflower oil is a major issue for the olive oil industry. In this paper, the potential of total synchronous fluorescence (TSyF) spectra to differentiate virgin olive oil from sunflower oil and synchronous fluorescence (SyF) spectra combined with multivariate analysis to assess the adulteration of virgin olive oil are demonstrated. TSyF spectra were acquired by varying the excitation wavelength in the region 270-720 nm and the wavelength interval (Deltalambda) in the region from 20 to 120 nm. TSyF contour plots for sunflower, in contrast to virgin olive oil, show a fluorescence region in the excitation wavelength range 325-385 nm. Fifteen different virgin olive oil samples were adulterated with sunflower oil at varying levels (0.5-95%) resulting in one hundred and thirty six mixtures. The partial least-squares regression model was used for quantification of the adulteration using wavelength intervals of 20 and 80 nm. This technique is useful for detection of sunflower oil in virgin olive oil at levels down to 3.4% (w/v) in just two and a half minutes using an 80-nm wavelength interval.

Olive Oil↗

Comparison of the effects of supplemental red palm oil and sunflower oil on maternal vitamin A status.

BACKGROUND: Conflicting results have been reported on the ability of dietary carotenoids to improve vitamin A status in lactating women. Red palm oil is one of the richest dietary sources of beta-carotene. OBJECTIVE: We aimed to determine the efficacy of red palm oil in increasing retinol and provitamin A status in pregnant and lactating women. DESIGN: Ninety rural, pregnant Tanzanian women from 3 randomly selected villages were recruited during their third trimester to participate in 3 dietary intervention groups: a control group, who were encouraged to maintain the traditional practice of eating staples with dark-green leafy vegetables, and 2 study groups, who were given either sunflower or red palm oil for use in household food preparations. The intervention lasted 6 mo. Plasma samples were collected at the third trimester and 1 and 3 mo postpartum, and breast-milk samples were collected 1 and 3 mo postpartum. RESULTS: Supplementation with red palm oil, which is rich in provitamin A, increased alpha- and beta-carotene concentrations significantly (P < 0.001) in both plasma and breast milk. Plasma retinol concentrations were similar in all dietary groups. Breast-milk retinol concentrations tended to decrease from 1 to 3 mo postpartum in the control group, but were maintained in both oil groups. The difference in change in breast-milk retinol concentration between the red palm oil group and the control group was significant (P = 0.041). CONCLUSIONS: Consumption of red palm oil increases concentrations of alpha- and beta-carotene in both breast milk and serum and maintains breast-milk retinol concentrations. Sunflower oil consumption seems to conserve breast-milk retinol similarly to consumption of red palm oil. Breast-milk retinol might be maintained through increased dietary intake of these vegetable oils and use of mild cooking preparation methods (such as the addition of oil at the end of cooking and avoidance of frying).

Adult↗

Butter differs from olive oil and sunflower oil in its effects on postprandial lipemia and triacylglycerol-rich lipoproteins after single mixed meals in healthy young men.

Accumulation of postprandial triacylglycerol-rich lipoproteins is generated by assimilation of ingested dietary fat and has been increasingly related to atherogenic risk. Nevertheless, the influence of different kinds of dietary fatty acids on postprandial lipid metabolism is not well established, except for (n-3) polyunsaturated long-chain fatty acids. Our goal was to evaluate the effects of test meals containing a common edible fat source of saturated (butter), monounsaturated (olive oil) or (n-6) polyunsaturated (sunflower oil) fatty acids on postprandial lipid and triacylglycerol-rich lipoprotein responses. After a 12-h fast, 10 healthy young men ingested mixed meals containing 0 g (control) or 40 g fat, provided as butter, olive oil or sunflower oil in a random order. Fasting and postmeal blood samples were collected for 7 h. The no-fat test meal did not elicit any change over baseline except for plasma phospholipids, insulin and nonesterified fatty acids. Conversely, the three fat-containing meals elicited bell-shaped postprandial changes (P < 0.05) in serum triacylglycerols, free and esterified cholesterol, and nonesterified fatty acids. The butter meal induced a lower postprandial rise of triacylglycerols in serum and chylomicrons (incremental AUC, mmol.h/L: 0.72) than the two unsaturated oils (olive oil: 1.6, sunflower oil: 1.8), which did not differ. Circulating chylomicrons were smaller after the butter meal than after the two vegetable oil meals. The in vitro susceptibility of circulating chylomicrons to hydrolysis by postheparin plasma was higher after sunflower oil than after butter or olive oil. We conclude that butter results in lower postprandial lipemia and chylomicron accumulation in the circulation of young men than olive or sunflower oils after consumption of a single mixed meal.

Adult↗

Blood pressure in spontaneously hypertensive rats fed butterfat, corn oil, or fish oil.

Dietary fats have been shown to influence blood pressure in humans and animal models of hypertension. The ability of a particular fat to modulate arterial pressure appears to depend on its fatty acid profile rather than its degree of saturation or unsaturation. Little is known about the effects of specific dietary fats of animal origin on blood pressure. We tested the concurrent effects of both calcium and dietary fat on blood pressure development in the spontaneously hypertensive rat. Sixty animals were fed diets containing butterfat, fish oil, or corn oil from 3-26 weeks of age. Each diet among the three oils was further modified to contain either 0.25% or 2.0% of the diet as calcium. All six diets provided 18% of the diet (36% of the calories) as fat. The polyunsaturated-to-saturated fat ratio was 0.07, 0.84, and 4.54 for butterfat, fish oil, and corn oil, respectively. Fish oil consumption resulted in lower blood pressures compared with butterfat (p less than 0.036) or corn oil (p less than 0.0009). Similarly, butterfat feeding resulted in lower blood pressures when compared with corn oil (p less than 0.054). Supplementing the diet with calcium decreased blood pressure in both the butterfat and corn oil diets. When butterfat diets were supplemented with calcium, the resulting blood pressures did not differ significantly from those obtained with the two fish oil diets. It is concluded that butterfat, though highly saturated, is associated with less of an increase in the spontaneously hypertensive rat's blood pressure than is corn oil, which is highly unsaturated.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

Effects of coconut oil, butter, and safflower oil on lipids and lipoproteins in persons with moderately elevated cholesterol levels.

The physiological effects of coconut oil, butter, and safflower oil on lipids and lipoproteins have been compared in moderately hypercholesterolemic individuals. Twenty eight participants (13 men, 15 women) followed three 6-week experimental diets of similar macronutrient distribution with the different test fats providing 50% total dietary fat. Total cholesterol and low density lipoprotein cholesterol were significantly higher (P < 0.001) on the diet containing butter [6.8 +/- 0.9, 4.5 +/- 0.8 mmol/l] (mean +/- SD), respectively than on the coconut oil diet (6.4 +/- 0.8; 4.2 +/- 0.7 mmol/l) when levels were significantly higher (P < 0.01) than on the safflower diet (6.1 +/- 0.8; 3.9 +/- 0.7 mmol/l). Findings with regard to the other measures of lipids and lipoproteins were less consistent. Apolipoprotein A-I was significantly higher on coconut oil (157 +/- 17 mg/dl) and on butter (141 +/- 23 mg/dl) than on safflower oil (132 +/- 22 mg/dl). Apolipoprotein B was also higher on butter (86 +/- 20 mg/dl) and coconut oil (91 +/- 32 mg/dl) than on safflower oil (77 +/- 19 mg/dl). However gender differences were apparent. In the group as a whole, high density lipoprotein did not differ significantly on the three diets whereas levels in women on the butter and coconut oil diet were significantly higher than on the safflower oil diet. Triacylglycerol was higher on the butter diet than on the safflower and coconut oil diets but the difference only reached statistical significance in women. Cholesteryl ester transfer activity was significantly higher on butter than safflower oil in the group as a whole and in women.(ABSTRACT TRUNCATED AT 250 WORDS)

Butter↗

The fate of oil and oil-dispersant mixtures in freshwater ponds.

The fate, distribution and composition of oil and oil-dispersant mixtures were studied in a series of five, lined, inground ponds containing sandy gravel sediment and mesotrophic water. Normal Wells crude oil and Corexit 9527 were added at nominal concentrations of 100 and 20 ppm, respectively, to two of the ponds, and the crude oil alone was added at 100 ppm to a third pond. The water surface, water column, the sediment, pond liner and attached biota were systemically sampled for a year. While only about 2% of the oil remained in the water column of the pond with no dispersant addition, in the pond with the dispersant, about 10% of the oil persisted in the water for several weeks. Most of the oil initially dispersed in the water returned to the water surface, then eventually sank to the sediment. Thinner surface films showed a higher dispersant content than the thicker slicks , and the thinner films had higher infrared carbonyl absorption. Final distribution calculations revealed that about 45% of the oil had degraded in the oil-dispersant-treated ponds during the one year study, while only 23% could not be accounted for in the oil pond. Changes in the oil composition during the experiment were similar in all ponds, with no evidence to suggest that the dispersant affected oil composition in any special manner.

Fresh Water↗