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[Role of the mesothelium of the parietal peritoneum in the process of absorption of true solutions and india ink suspension from the abdominal cavity].

The method of electron microscopy was applied to the study of absorption from the abdominal cavity of hypotoanic, physiological, hypertonic solutions and of the Indi-ink suspension on physiological saline. Quantitative assessment of pynocytosis was conducted on electron microphotographs. Passage through the mesothelium of true solutions was realized by way of diffusion through the cytoplasm of its cells and by the intercellular spaces; as to the India ink suspension, it passed only by way of the open intercellular spaces. Pynocytosis in the mesothelial cells coursed from their basal portion to the apical one and was directed to the balancing of the concentration of the protein and salt composition in the serous fluid.

Animals↗

Proposed standard methods for ink identification.

Two methods are presented for the identification of ink, both of which are currently being used by laboratories involved in ink analysis. Both methods incorporate physical and chemical procedures. The differences are primarily in chemical methods involving spot tests and thin layer chromatographic (TLC) techniques. Furthermore, one method utilizes spectrophotometric scanning of the TLC plate whereas the other method utilizes solution spectrophotometry. It is recommended that both methods be evaluated by several laboratories.

Chemical Phenomena↗

[Application of preoperative marking with Chinese-ink or methylenum coeruleum in radical operation of the esophagus (cardia). A preliminary study].

We present a method of staining with Chinese-Ink or methylenum coeruleum under endoscope preoperatively and its clinical valve as a guide for radical resection of esophagus (cardia) cancer. There were 39 cases of esophageal cancer and 9 cases of gastric cardia cancer. 37 cases were stained with Chinese-ink and 11 cases with methylenum coeruleum. 268 of the 334 groups (427 of 592 lymph modes) were stained. In the 73 metastatic groups (107 lymph nodes) 65 groups (96 lymph nodes) were stained (88.1%, 96/107). In this series radical operation was achieved in 45 cases. The indication of staining, choice of marking materials and main points of manipulation practice were discussed.

Adenocarcinoma↗

The cutaneous ink sign: a tipoff to suicide or suicide notes.

The presence of smeared or unsmeared ink on the skin resulting from the writing process has been observed in a number of suicide victims who have left suicide notes. The authors report a case of a palmar "cutaneous ink sign" and discuss its forensic science import.

Adult↗

Allergenicity of trimethylol propane triacrylate in ultraviolet curing inks in the guinea pig.

Trimethylol propane triacrylate (TMPTA) is a multifunctional acrylate commonly used in ultraviolet (UV) curing inks. Six men developed dermatitis when working with such UV curing inks in printing plants and all had positive patch test to TMPTA. The sensitizing capacity of TMPTA as determined by the "Guinea pig maximization test" (GMP) showed it to be a strong allergen. Cross-reaction with penta-erythritol triacrylate (PETA) occurs, but not with trimethylol propane trimethacrylate (TMPTMA).

Acrylates↗

Sensitization capacity of acrylated prepolymers in ultraviolet curing inks tested in the guinea pig.

One commonly used prepolymer in ultraviolet (UV) curing inks is epoxy acrylate. Of 6 men with dermatitis contracted from UV-curing inks, 2 had positive patch test reaction to epoxy acrylate. None reacted to the chemically related bisphenol A dimethacrylate. The sensitization capacity of epoxy acrylate and bisphenol A dimethacrylate performed with the "Guinea pig maximization test" (GPM) shows epoxy acrylate to be an extreme sensitizer and bisphenol A dimethacrylate a moderate sensitizer. Cross-reaction between the two substances occurs. The epoxy resin oligomer MW 340 present in the epoxy acrylate also sensitized some animals.

Acrylates↗

Phototoxicity occurring during the manufacture of ultraviolet-cured ink.

Four workers employed in the manufacture of ultraviolet-cured inks complained of photosensitivity characterized by an intense burning sensation during sun exposure. Three of these workers developed dermatitis on exposed areas following sun exposure. Six compounds used as photoinitiators in the ink formulations were found to absorb solar ultraviolet radiation. Two preparations of mixed isomers (ortho and para) of amyl dimethylaminobenzoate were found to be phototoxic to Ehrlich ascites cells in vitro and to produce diphasic phototoxic reactions in vivo after topical application on symptomatic workers, asymptomatic workers, or previously unexposed subjects. These responses could be prevented in two subjects by the application of a 10% sulizobenzone sunscreen prior to sun exposure. Two other photoinitiators, Michler's ketone and thioxanthone were phototoxic in vitro but not after topical application in vivo.

Adult↗

New simple ink writing pen system for kymograph recording.

Extraordinarily simple, inexpensive and easily available kymograph ink writing pen system for physiological and pharmacological recordings has been described. The writing part of the pen, consisting of a small porous fibrous writing rod to which a small spongy absorbing cylinder (as an ink container) is added, is attached to one end of a very light plastic strip of which opposite end is connected to a common mechanical lever. The function of the pen was investigated by recording spontaneous frog heart contractions in situ. The pen worked quite well and the recordings were reproducible. The pen can be interesting for researchers working in those physiology and pharmacology laboratories where more sophisticated recorders are not available, and is especially convenient for mass use in students' physiology and pharmacology labs, as alternative for smoked-drum kymograph method.

Animals↗

Occupational dermatitis to ultraviolet-cured acrylic-based inks in computer hard disc manufacturing.

OBJECTIVE: Ultraviolet-cured acrylates and their various components and applications are reviewed in this report. METHODS: A 26-year-old woman involved in silk screening computer discs with ultraviolet-cured inks developed an acute allergic contact dermatitis on her hands and forearms. Patch testing revealed a number of strong reactions to epoxy resin and many multifunctional acrylates. RESULTS: The only one listed on the material safety data sheet to which she reacted was tripropyleneglycol diacrylate (TRPGDA). The other positive reactions likely represent cross-reactions. The positive reaction to epoxy is possibly relevant and attributable to nonhardened epoxy resin contaminant in the epoxy prepolymer. CONCLUSION: This case report shows a new application for ultraviolet-cured acrylate based inks in computer hard disc manufacturing.

Acrylates↗

The combination of Indian ink staining with immunochemiluminescence detection allows precise identification of antigens on blots: application to the study of glycosylated barley storage proteins.

The localization after blotting of specific spots in two-dimensional electrophoretic protein pattern was achieved using, in that order, Indian ink protein staining and immunodetection with chemiluminescence on the same membrane. Indian ink did not inhibit significantly the antibody reactions even after overnight staining. It produces permanent staining that did not quench the chemiluminescent signal, recorded on a film. This allowed perfect matching between the specific and the total protein patterns. The procedure was applied to the identification of glycoproteins present in barley storage protein preparations.

Antigens↗

Preparation of Magnetic Inks: Adsorption of Macromolecular Dispersion Agents on Magnetic Metallic Iron Pigments and Electrical Charge Formation

The interaction between metallic iron pigments (MPs) and the actual constituents of magnetic ink is examined by first determining the adsorption isotherms and analyzing by FTIR the type of bonding that is established between the constituents and MP. Then, following the approach described in a previous paper using model compounds, the formation of electrical charges on MPs is examined through acoustophorometric (Electrokinetic amplitude or ESA) measurements. It is shown that the variation in ESA is related to charge transfer processes and reflects the MP-constituent interactions. Finally, adsorption competitions are shown and the importance of the method of preparation (order of mixing of the ingredients of the ink) is illustrated. Copyright 1997 Academic Press. Copyright 1997Academic Press

Journal Article↗

The healing of femoral neck fractures in dogs. The use of intraosseous venography and India ink staining.

This study evaluates healing fractures of the femoral neck in dogs by using standard radiography, intraosseous venography, India ink staining and histology. The 45 animals were divided into three groups, one of which was not treated, but in the other two the fracture was fixed with a screw or two Kirschner wires. Our results suggest that, when intraosseous venography was positive India ink injection was also positive, and the late phase of fracture healing was reached.

Animals↗

Examination of the contaminants and performance of animals fed and bedded using de-inking paper sludge.

In the noncereal-producing areas, there is not enough straw to supply the amount required for animal bedding. However, pulp and paper mills produce tons of wood fiber wastes, including de-inking paper sludge (DPS), in which most toxic compounds are at their detection limit. Among the detected compounds in DPS, aluminum, copper, and polycyclic aromatic hydrocarbons (PAHs) are present and were selected as model molecules. In this context, broilers were submitted to a diet containing 0, 5, and 10% of their ration as DPS. In addition, broilers and pigs were grown on de-inking paper sludge and wood shavings beddings. The presence of aluminum and copper were evaluated in blood and bones, whereas the presence of PAHs was evaluated in fat, liver, meat, and urine or blood of broilers and pigs. Animal performances were also investigated. DPS bedding did not increase aluminum or copper contents of blood or bones or PAHs in animal tissues. Animal performances and health were similar on DPS and wood shaving beddings. Using DPS as bedding material provides an integrated source of disposal of DPS and animal manure.

Aluminum↗

Unusual course of colonic tattooing with India ink.

We here report the successful but unusual course of colonic tattooing in a patient with a carcinoma in situ in a polyp. A 70-year-old woman was admitted for persistent diarrhea, occult fecal blood, and anemia. During colonoscopy, a pedunculated large polyp in the sigmoid colon occluding the lumen was removed successfully. Histopathological examination revealed a carcinoma in situ with a resection of the peduncle in sano. At the second sigmoidoscopy, the polypectomy site was marked with India ink to facilitate the recovery of the polypectomy site in follow-up endoscopies. Three months later, the India ink had spread in the submucosal layer and a segment measuring 15 cm was colored dark blue. At the original polypectomy site, an uncolored flat mucosal proliferation was found above the dark colonic wall. After mucosectomy, the tissue was classified as hyperplastic. Six weeks later, a second control sigmoidoscopy did not show any suspicious mucosal alterations.

Adenoma, Villous↗

Tailor-made tricalcium phosphate bone implant directly fabricated by a three-dimensional ink-jet printer.

Rapid prototyping (RP) is a molding technique that builds a three-dimensional (3D) model from computer-aided design (CAD) data. We fabricated new tailor-made bone implants (TIs) from alpha-tricalcium phosphate powder using an RP ink-jet printer based on computed tomography (CT) data, and evaluated their safety and efficacy. CT data of the skulls of seven beagle dogs were obtained and converted to CAD data, and bone defects were virtually made in the skull bilaterally. TIs were designed to fit the defects and were fabricated using the 3D ink-jet printer with six horizontal cylindrical holes running through the implants, designed for possible facilitation of vascular invasion and bone regeneration. As a control, hydroxyapatite implants (HIs) were cut manually from porous hydroxyapatite blocks. Then, craniectomy was performed to create real skull defects, and TIs and HIs were implanted. After implantation, CT was performed regularly, and the animals were euthanized at 24 weeks. No major side effects were observed. CT analysis showed narrowing of the cylindrical holes; bony bridging between the implants and the temporal bone was observed only for TIs. Histological analysis revealed substantial new bone formation inside the cylindrical holes in the TIs, while mainly connective tissues invaded the porous structures in HIs. Bone marrow was observed only in TIs. Osteoclasts were seen to resorb regenerated bone from inside the cylindrical holes and to invade and probably resorb the TIs. These data suggest that TIs are a safe and effective bone substitute, possessing osteoconductivity comparable with that of HIs.

Animals↗

Fixation increases sensitivity of India ink staining of proteins and peptides on nitrocellulose paper.

The detectability by India ink staining of proteins and peptides dot-blotted on nitrocellulose paper was assessed before and after fixation. Fixation considerably increased the detectability of proteins and peptides. Denaturation by KOH treatment or baking at 100 degrees C for 15 min gave the best results. Precipitation by isopropanol/acetic acid gave intermediate results, whereas crosslinking with glutaraldehyde improved the detectability of small peptides, but not of proteins. Ferridye and Aurodye were also tested after baking. Both dyes were more sensitive and stained more proteins and peptides than India ink. In all cases the detectability of peptides smaller than Mr 1500 was poor.

Carbon↗

Transfer characteristics of an ink-jet writer for cardiovascular studies.

The transfer characteristic of a 16 channel ink-jet writer used in a cardiovascular laboratory is described. The test method for measuring linearity, gain and phase response is described and results given. The transfer function was calculated from the normalized experimental results and allows prediction of the behaviour of the writer for different input signals. It is shown that the ink-jet galvanometers are linear for signal input amplitudes between 0 and 2.5 V, and have a flat frequency response between DC and 640 Hz (+/- 1%).

Biomedical Engineering↗

To boost or not to boost: decreasing radiation therapy in conservative breast cancer treatment when "inked" tumor resection margins are pathologically free of cancer.

A retrospective study was performed to compare local treatment approaches for 108 treated breasts in 105 patients with Stage I or II breast cancer. Six cases with intraductal carcinoma have shown no evidence of recurrence. The other 102 cases had invasive cancer. In 54 treated breasts in 53 patients, the treatment approach involved surgical resection of the primary tumor, pathological determination of tumor-free "inked" specimen margins and 5000 cGy to the whole breast. Local radiation therapy (RT) boosts to the primary site were not given. This approach produced a 100% local control rate (mean follow-up of 38 months). In 28 treated breasts in 27 patients, the treatment approach involved tumor excision without evaluation of specimen margins followed by RT which included a local boost by either interstitial Iridium-192 implant or electron beam. This approach yielded an actuarial local control rate of 87% at 48 months (mean follow-up of 47 months). The difference in local control rate between the two groups was statistically significant (p less than 0.03). Among patients with clear surgical margins who received a local RT boost, 1 of 9 developed a local recurrence. Among those with tumor involving specimen margins who received a local boost, 1 of 8 developed local recurrence. Local recurrence developed more frequently among patients with poorly differentiated cancers (2 of 11 cases) than among those with other invasive cancers (3 of 91 cases). Comparison of treatment approaches was limited since poorly differentiated cancer was present in 25% of cases with unknown specimen margins, as compared with only 2% of those with clear surgical margins who did not receive a local RT boost. Our preliminary findings suggest that when "inked" primary tumor resection margins are pathologically free of cancer, 5000 cGy whole breast RT appears to be highly effective for local tumor control in patients with Stage I or II disease. Our results are inconclusive as to whether patients with poorly differentiated cancers should receive a local RT boost even when surgical margins are clear.

Actuarial Analysis↗