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PubMedDate not supplied

Sites of plasticity in the neural circuit mediating tentacle withdrawal in the snail Helix aspersa: implications for behavioral change and learning kinetics.

The tentacle withdrawal reflex of the snail Helix aspersa exhibits a complex combination of habituation and sensitization consistent with the dual-process theory of plasticity. Habituation, sensitization, or a combination of both were elicited by varying stimulation parameters and lesion condition. Analysis of response plasticity shows that the late phase of the response is selectively enhanced by sensitization, whereas all phases are decreased by habituation. Previous data have shown that tentacle withdrawal is mediated conjointly by parallel monosynaptic and polysynaptic pathways. The former mediates the early phase, whereas the latter mediates the late phase of the response. Plastic loci were identified by stimulating and recording at different points within the neural circuit, in combination with selective lesions. Results indicate that depression occurs at an upstream locus, before circuit divergence, and is therefore expressed in all pathways, whereas facilitation requires downstream facilitatory neurons and is selectively expressed in polysynaptic pathways. Differential expression of plasticity between pathways helps explain the behavioral manifestation of depression and facilitation. A simple mathematical model is used to show how serial positioning of depression and facilitation can explain the kinetics of dual-process learning. These results illustrate how the position of cellular plasticity in the network affects behavioral change and how forms of plasticity can interact to determine the kinetics of the net changes.

Animals↗
PubMed1999

The actions of FxRFamide related neuropeptides on identified neurones from the snail, Helix aspersa.

Intracellular recordings were made from identified neurones in the suboesophageal ganglia of the snail, Helix aspersa. The actions of the eight FxRFamide analogues were investigated on these neurones. These peptides included ones isolated from arthropods and nematodes. All the peptides excited certain neurones while inhibiting others, though their relative potencies varied. Overall on neurones inhibited by these peptides the potency order was: DNFLRFamide > FMRFamide > PDVDHVFLRFamide = KNEFIRFamide > FLRFamide >> SDRNFLRFamide = SDPNFLRFamide > KHEYLRFamide. However, if the responses are compared on individual cell types, then the picture becomes more complex. For example, on cell F-2, KNEFIRFamide proved to be potent with an EC-50 value of 0.54 microM. On neurones F-13/16 and E-16, PDVDHVFLRFamide was inhibitory while FMRFamide, FLRFamide, SDRNFLRFamide and SDPNFLRFamide were excitatory. In terms of overall excitatory actions, the data are less complete but an approximate order of potency is: FMRFamide > DNFLRFamide >> SDPNFLRFamide > PDVDHVFLRFamide >> KNEFIRFamide = KHEYLRFamide = SDRNFLRFamide. However this again varies between specific neurones. These results demonstrate that peptides from insects, crustacea and nematodes are active on Helix neurones and may activate specific receptor subtypes, indicating the possible presence of endogenous analogues of these non-molluscan peptides in the Helix nervous system.

Amides↗
PubMed1987

Interactions among amino acid transport systems in snail Helix aspersa intestine.

Interactions among the transport of diverse amino acids in everted intestine of snail Helix aspersa have been studied. The uptake of 0.5 mM methionine is clearly inhibited by high concentrations (40 mM) of leucine, and not by proline or lysine, whereas the last two amino acids inhibit cycloleucine uptake. Methionine strongly inhibits proline and lysine uptake, which is significantly inhibited by their analogs hydroxiproline and arginine, respectively. Results suggest that in Helix intestine the transport systems for basic amino acids and iminoacids are shared with high affinity by methionine whereas the neutral amino acids transport systems do not seem to be shared, or are so very weakly, by the basic ones or by the imino acids.

Animals↗
PubMed1996

Effect of Zn, Cu, Pb, and Cd on fitness in snails (Helix aspersa).

Juveniles and adults of the Brown garden snail (Helix aspersa Müll.) were fed on an artificial diet contaminated with Zn (ca. 40-12,000 micrograms.g-1 dry wt), Cu (ca. 9-1600 micrograms.g-1 dry wt), pb (ca. 0.4-12,700 micrograms.g-1 dry wt), Cd (ca. 0.16-145 micrograms.g-1 dry wt), and all four metals mixed together for 120 days. Significant negative exponential regressions of food consumption and fecundity on concentrations were found for all treatments. Growth rate was affected significantly only by Zn and mixed treatments. The calculated EC20(consumption) values for juveniles were (in microgram.g1): Zn, 855; Cu, 248; Pb, 5290; Cd, 60; and for adults: Zn, 1240; Cu, 275; Pb, 3120; Cd, 147. In mixed treatment EC20(consumption) values were substantially lower indicating the additive effect of pollution with these four metals. EC20(consumption) for Zn in mixed treatment was 329 micrograms.g-1 for juveniles and 661 micrograms.g-1 for adults. The following EC20 values were estimated for fecundity (in micrograms.g-1): Zn, 1740; Cu, 533; Pb, 6140; Cd, 120; Zn in mixed treatment, 2210. The relative toxicities of the four metals were compared with their ratios in contaminated field sites. Comparing Zn and Cd, for example, even though Zn is ca. 13-24 times less toxic than Cd, it is usually present in plants and forest litter in concentrations ca. 100 times greater than those of Cd. Thus, of these two metals, Zn appeared to be potentially the most important pollutant in ecologically relevant situations. No effect of any treatment on mortality was found during the 4-month experiment. The calculated scenarios of population dynamics under the stress of chronic pollution with mixtures of the four metals revealed that the delayed reproduction due to estivation of snails may be the main cause of population decline at high metal concentrations in food. However, at concentrations at and below ca. 1000 micrograms Zn.g-1 in food, if the reproduction is not delayed the population may persist for a long time (0.25 control number after 50 years).

Aging↗
PubMed1999

Topographic organization of serotonergic and dopaminergic neurons in the cerebral ganglia and their peripheral projection patterns in the head areas of the snail Helix pomatia.

The distribution of monoaminergic neurons within the cerebral ganglia was investigated in the pulmonate snail Helix pomatia. Simultaneous serotonin and tyrosine hydroxylase double immunostaining revealed that the immunoreactive cell groups are concentrated in a putative monoaminergic center on the ventral surface of the cerebral ganglia. Simultaneous cobalt (Co)- and nickel (Ni)-lysine backfills of cerebral nerves were combined with 5, 6-dihydroxytryptamine pigment-labelling of serotonergic neurons, or with fluorescence immunocytochemistry of dopaminergic neurons. This showed that the serotonergic and dopaminergic cell groups can be divided into smaller subgroups on the basis of their axonal projections into different cerebral nerves. These subgroups show a topographic organization within the serotonergic and dopaminergic neuronal clusters. In the serotonergic system, the different regions of the head are represented in a rostrocaudal direction, whereas a caudorostral organization is characteristic for the dopaminergic system. No serotonin- or dopamine-immunoreative cell bodies but numerous fibers were observed in the head areas, indicating that these are innervated by cerebral monoaminergic neurons and show different innervation patterns. Serotonin-immunoreactive fibers mostly innervate muscle fibers, whereas dopamine-immunoreactive processes do not innervate effector cells, but terminate within the nerve branches of the head areas. On the basis of their innervation pattern, we suggest that dopaminergic neurons may take part in en route modulation of sensory afferent and efferent processes in an as yet unknown manner. The serotonergic neurons, on the other hand, may play a direct role in the modulation of muscle function.

Animals↗
PubMed1984

The possible site of action of 5-hydroxytryptamine, 6-hydroxytryptamine, tryptamine and dopamine on identified neurons in the central nervous system of the snail, Helix aspersa.

Intracellular recordings were made from identified neurons in the right parietal ganglion of the snail, Helix aspersa. Cells F 4, 5 and 6 were excited by 5-hydroxytryptamine (5-HT) and inhibited by dopamine while cells in the F 30 area were inhibited by both compounds. Low doses of both tryptamine and 6-HT produced weak excitation of cells F 4, 5 and 6 while higher doses of both compounds inhibit the activity of these cells. In terms of the inhibitory responses, tryptamine and 6-HT are approximately equipotent but between 10 and 100 times less potent than dopamine. d-Tubocurarine reversibly antagonized the excitatory action of 5-HT on cells F 4, 5 and 6 and converted tryptamine and 6-HT excitation to inhibition. In the presence of the antagonist, ergometrine, the dopamine inhibitory response was almost completely abolished while the inhibitory responses to tryptamine and 6-HT were converted to weak excitation. All four agonists inhibited cells in the F 30 area with the following potency ratios: dopamine much greater than tryptamine/6-HT greater than 5-HT. Tubocurarine had no antagonist effects on these responses while ergometrine reduced or blocked all four, often irreversibly. In potassium-free Ringer the inhibitory responses to all four agonists were enhanced. It is concluded that on cells F 4, 5 and 6, low concentrations of tryptamine and 6-HT act on 5-HT receptors while higher concentrations of both agonists act on dopamine receptors. On cells in the F 30 area, 5-HT, 6-HT and tryptamine all act on a dopamine receptor.

Animals↗
PubMed1986

The ionic mechanism associated with the action of putative transmitters on identified neurons of the snail, Helix aspersa.

Intracellular recordings were made from identified neurons in the suboesophageal ganglionic mass of the snail, Helix aspersa. The ionic mechanisms associated with acetylcholine excitation and inhibition, dopamine excitation and inhibition, gamma-aminobutyric acid (GABA) excitation and inhibition and serotonin excitation were investigated. Acetylcholine excitation was found to involve an initial increase in sodium conductance while acetylcholine inhibition was a pure chloride event which reversed at membrane potentials more negative than the chloride equilibrium potential. Dopamine excitation appeared to involve only an increase in sodium conductance while serotonin excitation involved an increase in conductance to both sodium and calcium ions. Dopamine inhibition was associated with an increase in potassium conductance but failed to reverse at membrane potentials more negative than the potassium equilibrium potential. GABA excitation involved conductance increases to both sodium and chloride ions while GABA inhibition was a pure chloride event. An attempt was made to estimate the degree of co-operativity of the putative transmitters with their receptors using log-log and Hill plots. The slopes of the line for the log-log plots for acetylcholine excitation and inhibition were 0.88 and 1.1, respectively, suggesting the interaction of one molecule of acetylcholine with the receptor. The slope of the log-log plot for dopamine inhibition was 0.46 while that for serotonin excitation was 0.75. The Hill plots for GABA excitation and inhibition were 1.64 and 1.42, respectively, suggesting that two molecules of GABA are required for receptor activation.

Acetylcholine↗
PubMed1975

Thermal susceptibility of Salmonella in the Moroccan food snail, Helix aspersa.

Thirty samples of 10-15 Helix aspersa food snails were examined for Salmonella by a surface rinsing method and by analysis of whole snails rinsed with 70% ethanol. Thirteen samples were positive by the rinsing method and 6 were positive by whole snail analysis, with this difference being significant (P less than 0.01). Although Salmonella contamination in H. aspersa appeared to be predominantly surface, the pathogen was also found within the snail meat. The ability of surface and subsurface Salmonella organisms to survive in cooked snails was determined in 90 samples. Thermocouple readings indicated that an internal temperature of at least 200 degrees F (93 degrees C) was reached within the snail meat during cooking by a typical recipe. This temperature was sufficient to kill both surface and subsurface Salmonella in 29 samples positive for the pathogen prior to heating. A variety of serotypes representing several somatic groups was isolated.

Food Contamination↗
PubMed1992

Actions of APGW-amide and GW-amide on identified central neurons of the snail, Helix aspersa.

1. Actions of APGWamide and GWamide have been examined on identified central neurons of the snail, Helix aspersa. 2. In F1 neurons, both APGWamide and GWamide, 0.5-5 microM, reversibly inhibited the amplitude of evoked-IPSPs which were dopaminergic, but had no direct effect on membrane potential, cell firing rate, or dopamine-induced responses. These results indicate that the actions of APGWamide and GWamide in F1 neurons are at presynaptic sites. 3. At concentrations between 0.5 and 10 microM, APGWamide and GWamide had direct postsynaptic effects on F2 neurons. They inhibited the spike activity and hyperpolarized the membrane potential of F2 neurons in a dose-dependent manner with a reversal potential around -85 mV which is close to Ek. 4. In K+ free solution, the inhibitory effects of APGWamide and GWamide were potentiated, while they were reduced by increasing external K+ concentration. Either tetraethylammonium (10 mM) or 4-aminopyridine (500 microM) only partially prevented the inhibition induced by APGWamide and GWamide on F2 neurons. Combination of TEA (5 mM) and 4-AP (250 microM) could abolish this inhibition. However, neither 1 mM La2+ nor 10 mM Co2+ could prevent the inhibitory action of APGWamide and GWamide. This evidence indicates that the postsynaptic inhibition of APGWamide and GWamide on F2 neurons is due to an increase in K+ conductance and that both transient K channel (IA) and delay K+ channel (IK) were affected by these peptides. 4. APGWamide and GWamide exert both presynaptic and postsynaptic effects of Helix neurons, depending on the neuron under study. They are qualitatively and quantitatively similar in their presynaptic or postsynaptic actions.(ABSTRACT TRUNCATED AT 250 WORDS)

Amino Acid Sequence↗
PubMedDate not supplied

[The effect of glutamic acid on the defensive and feeding behavior of the snail Helix lucorum].

Behavioural effects of glutamic acid injection into the body cavity were investigated in the land snail Helix lucorum. An injection itself induced some non-coordinated defensive reactions leading to a partial withdrawal into a shell. The defensive tentacle reflex was facilitated probably due to an activation of the tentacle muscles. The defensive reflex of the pneumostome closure changed in different directions depending on the injected dose of glutamic acid. Feeding behaviour was inhibited by a high dose of glutamic acid. The most pronounced inhibition of the feeding behaviour was induced by N-methyl-DL-aspartic acid.

Animals↗
PubMed2004

Intracellular pH regulation in isolated hepatopancreas cells from the Roman snail (Helix pomatia).

The mechanisms of intracellular pH (pHi) regulation were studied in isolated hepatopancreas cells from the Roman snail, Helix pomatia. The relationship between intracellular and extracellular pH indicated that pHi is actively regulated in these cells. At least three pHi-regulatory ion transporters were found to be present in these cells and to be responsible for the maintenance of pHi: an amiloride-sensitive Na+/H+ exchanger, a 4-acetamido-4'-isothiocyanostilbene-2,2'disulfonic acid (SITS)-sensitive, presumably Na(+)-dependent, Cl-/HCO3-exchanger, and a bafilomycin-sensitive H(+)-pump. Inhibition of one of these transporters alone did not affect steady state pHi, whereas incubation with amiloride and SITS in combination resulted in a significant intracellular acidification. Following the induction of intracellular acidosis by addition of the weak acid Na+propionate, the Na+/H+ exchanger was immediately activated leading to a rapid recovery of pHi towards the baseline level. Both the SITS-sensitive mechanism and the H(+)-pump responded more slowly, but were of similar importance for pHi recovery. Measurement of pHi recovery from acidification in the three discernible types of hepatopancreas cells with a video fluorescence image system revealed slightly differing response patterns, the physiological significance of which remains to be determined.

Acid-Base Equilibrium↗
PubMedDate not supplied

Newly identified nerve cells of the snail, Helix pomatia, associated with the generation of pacemaker activity.

Data on new, previously unidentified nerve cells of the snail Helix pomatia are presented in this paper. The identified neurons described may serve as a convenient model for the investigation of the cellular mechanisms of pacemaker activity, the role of neuropeptides in the generation and regulation of pacemaker activity, peptidergic transmission, and the functional role of the inward calcium current.

Animals↗
PubMed1990

The reaction of nitrite with the haemocyanin of the Roman snail (Helix pomatia).

The reaction of nitrite at pH 5.0-7.0 with the deoxyhaemocyanin of a mollusc, the Roman snail (Helix pomatia), yielded nitrosylhaemocyanin (CuIA.NO+ CuIIB), in contrast with the formation of methaemocyanin with the deoxyhaemocyanin of the crustacean Astacus leptodactylus (mud crayfish). With Helix haemocyanin 1 NO was thereby liberated per active site, as shown by m.s., as against 2 NO with Astacus haemocyanin. Helix nitrosylhaemocyanin was characterized in c.d. by the negative extremum at 336 nm (CuIA.NO+) and by the mononuclear e.p.r. signal at g = 2 (CuIIB). Binuclear e.p.r. signals have been observed after the addition of nitrite to methaemocyanins. With Astacus methaemocyanin, no further reaction occurred, whereas with Helix methaemocyanin the mononuclear e.p.r. signal, characteristic for nitrosylhaemocyanin gradually appeared. This formation of Helix nitrosylhaemocyanin implicates the binding, most likely on CuIIA, of a second nitrite besides a bridging nitrite, so that a dismutation into NO and NO2 can occur there. A further dismutation of NO2 yields nitrite and nitrate. The formation of the latter was demonstrated by Raman spectrometry. The reaction rate of Helix methaemocyanin with nitrite decreased with increasing pH according to the Henderson-Hasselbalch equation with a pKa value of 6.77, attributed to a mu-aquo bridging ligand, which can be exchanged for nitrite, in equilibrium with a mu-hydroxo ligand which cannot. These data also favour the formulation of the final reaction product as nitrosylhaemocyanin instead of semi-methaemocyanin, with or without bound nitrite.

Animals↗
PubMed2000

Processes contributing to metabolic depression in hepatopancreas cells from the snail Helix aspersa.

Cells isolated from the hepatopancreas of the land snail Helix aspersa strongly depress respiration both immediately in response to lowered P(O2) (oxygen conformation) and, in the longer term, during aestivation. These phenomena were analysed by dividing cellular respiration into non-mitochondrial and mitochondrial respiration using the mitochondrial poisons myxothiazol, antimycin and azide. Non-mitochondrial respiration accounted for a surprisingly large proportion, 65+/-5 %, of cellular respiration in control cells at 70 % air saturation. Non-mitochondrial respiration decreased substantially as oxygen tension was lowered, but mitochondrial respiration did not, and the oxygen-conforming behaviour of the cells was due entirely to the oxygen-dependence of non-mitochondrial oxygen consumption. Non-mitochondrial respiration was still responsible for 45+/-2 % of cellular respiration at physiological oxygen tension. Mitochondrial respiration was further subdivided into respiration used to drive ATP turnover and respiration used to drive futile proton cycling across the mitochondrial inner membrane using the ATP synthase inhibitor oligomycin. At physiological oxygen tensions, 34+/-5 % of cellular respiration was used to drive ATP turnover and 22+/-4 % was used to drive proton cycling, echoing the metabolic inefficiency previously observed in liver cells from mammals, reptiles and amphibians. The respiration rate of hepatopancreas cells from aestivating snails was only 37 % of the control value. This was caused by proportional decreases in non-mitochondrial and mitochondrial respiration and in respiration to drive ATP turnover and to drive proton cycling. Thus, the fraction of cellular respiration devoted to different processes remained constant and the cellular energy balance was preserved in the hypometabolic state.

Adenosine Triphosphate↗
PubMed1986

Localization of a substance P-like material in the central and peripheral nervous system of the snail Helix aspersa.

A monoclonal antibody against substance P was used for immunocytochemical staining of the central ganglia of the snail Helix aspersa and several peripheral tissues including the gut, reproductive system, cardiovascular system, tentacle and other muscles. Within the central ganglia many neurons, and many fibres in the neuropile and the nerves entering the ganglia, were stained for the SP-like material. The largest numbers of reactive cell bodies were in the pleural ganglia and on the dorsal surfaces of the pedal ganglia. A group of cells was also found, surrounding the right pedal-cerebral connective, that did not fluoresce, but were enveloped by reactive processes terminating directly onto the neurone somata. Specific staining was observed in all peripheral tissues examined and always appeared to be concentrated in nerve terminals. Most particularly these occurred in the heart and aorta, the pharyngeal retractor muscle and the tentacle. Although mostly present in muscular tissues, some fluorescence was also observed in the nervous layer surrounding the retina. The tentacular ganglion also contained immunoreactive cell bodies.

Animals↗
PubMed1994

Carbonic anhydrase and CO2 chemoreception in the pulmonate snail Helix aspersa.

We have studied the effects of carbonic anhydrase inhibition on the hypercapnic ventilatory response of the pulmonate snail, Helix aspersa, in an isolated brain-pneumostome preparation. We found that the cell permeant carbonic anhydrase inhibitor, acetazolamide (ACTZ), increased pneumostomal opening and ventilation during normocapnia (2-3% CO2) and decreased the rate of pneumostomal response to step changes in CO2 (4.5%), but did not change the steady-state ventilatory response to elevated CO2 (4.5%) compared to the inactive ACTZ analogue, N2-substituted 2-acetylamino-1,3,4-thiadiazole (Cl 13850). In contrast, the cell impermeant carbonic anhydrase inhibitor, quartenary ammonium sulfonilamide (QAS), had no effect on the pneumostomal response to CO2 compared to Cl 13850. Using Hansson's histochemical technique to stain for carbonic anhydrase activity, we identified a small number of neurons in the subesophageal ganglia that exhibited carbonic anhydrase activity. Some of these cells were in the region of CO2-sensitivity. In conclusion, carbonic anhydrase inhibition slows the ventilatory response to rapid changes in CO2, but does not affect the intrinsic ability of H. aspersa to respond to CO2. The ventilatory effects of carbonic anhydrase inhibition may be attributed to the intracellular actions of the carbonic anhydrase enzyme.

Animals↗
PubMed1981

Light and electron microscopic investigation of the sensory cell bodies in the epidermis of the foot of the snail Helix pomatia L.

Light and electron microscopic studies were made on the receptor cells in the sole of the snail Helix pomatia. The cell bodies of the primary receptor cells, which can frequently be found in columnar or ganglion-like groups are situated at different depths below the basal lamina. The perikarya of the sensory cells usually measure 12 micrometers by 8 micrometers. Their nuclei are usually invaginated and contain large amounts of marginal heterochromatic substance. The nucleus usually measures 8 micrometers by 6 micrometers. The cytoplasm contains a well-developed Golgi apparatus consisting of several stacked lamellae surrounded by clear and dense-core vesicle. There are differences between cells in the amount of smooth and rough endoplasmic reticulum, in the free ribosomes and in other cytoplasmic elements. The axonal processes of the sensory cells are generally thin and connected to the subepithelial plexus. There are axonic swellings containing a mixed vesicle population, some of them near the muscle cell processes. We failed to find typical axo-axonic synaptic connections in the subepithelial nerve plexus as well as in motor connections within the muscle cell processes. On the basis of the ultrastructural features of the vesicles and other organelles we failed to classify different types of sensory cells. The ultrastructural difference between sensory cell bodies may be explained with a presumptive differentiation process.

Animals↗
PubMedDate not supplied

Pharmacological actions of essential (zinc, copper) and toxic metal ions (lead, mercury) on neurons and neuronal synapses of the snail, Helix pomatia L.

Actions of zinc, copper, lead and mercury ions on identified neurons and neuronal synapses of the snail, Helix pomatia L. were examined by use of electrophysiological methods. The studied metal ions depolarized the membrane and decreased the resistance but the neuronal excitability changed differentially. Copper and zinc transiently increased the amplitude and/or frequency of the spontaneous EPSPs or EPSCs in time- and dose-dependent ways. Lead attenuated the evoked EPSPs or EPSCs but copper and in some neurons zinc and mercury induced dose-dependent biphasic effects. Metal ions influenced the potential dependence, reversal potential, rate of rise and rate of fall of evoked responses differentially. The actions of zinc and lead on evoked EPSPs were reversible but those of copper and mercury were not and DTT treatment was ineffective. The affinity of the metal ions to the binding site(s) was low and the binding kinetics slow. The sensitivity of the binding site decreased in the order of mercury>copper>lead>zinc according to the IC50 values. The binding stoichiometry (nH value) changed from 1.4 to 2.6 for the various metal ions.

Animals↗