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At least 199 records · Page 11Linked to original sources
PubMed1992

[Development of serodiagnostic kit "HITAZYME Chlamydia Ab" for Chlamydia trachomatis infections using extracted antigen].

To develop EIA kit with low cross-reactivity for the quantitative detection of anti-chlamydial antibodies, we examined the preparation of trachomatis antigens and its specificity to mouse antisera and human sera. The chlamydial elementary body (EB) purified from C. trachomatis L2/434/Bu strain was treated by Sarkosyl, dithiothreitol and SDS by turns to obtain the soluble EB outer membrane (COMC). SDS-PAGE showed that the major components of the COMC were 96K, 60K and 39.5 KDa peptides. The reactivity of the COMC immobilized to 96 wells microtiter plate to mouse anti-serum to C. trachomatis was higher than the other two mouse anti-sera to C. psittaci and pneumoniae. In human sera, the cut off values were calculated from an average optical density plus its two-fold standard deviation obtained by the testing of 100 samples of healthy human sera. We evaluated the specificity of the kit to 17 anti-C. pneumoniae, 9 C. trachomatis and 4 C. psittaci antibodies positive patients' sera judged by the MFA method respectively. The results showed that the concordance ratio of IgG and IgA were 88%, 100% in anti-C. pneumoniae, 89%, 78% in anti-C. trachomatis and 50%, 50% in anti-C. psittaci respectively. From the results obtained in this study, we concluded that the HITAZYME method which had a very low cross-reactivity to C. pneumoniae is clinically useful in the serodiagnosis of C. trachomatis infections, even if it has a little common antigenicity with C. psittaci antigen.

Adolescent↗
PubMed1999

Detection of Chlamydia trachomatis endocervical infections by ligase chain reaction versus ACCESS Chlamydia antigen assay.

Ligase chain reaction (LCR) was compared with ACCESS immunoassay for detection of chlamydial infections in females. Despite efforts to improve ACCESS performance by evaluation of specimens that were in the test performance "grey zone," LCR remained more sensitive and was less expensive to perform. ACCESS had a sensitivity of 83.9%, a specificity of 99.7%, a positive predictive value of 96.3%, and a negative predictive value of 98.5%.

Antigens, Bacterial↗
PubMedDate not supplied

[Kinetic study of the antibodies in OF 1 and C57Bl mice infected with Chlamydia trachomatis and Chlamydia psittaci. Its application to the interpretation of the serological results in human chlamydioses].

The kinetics of antibodies to C. trachomatis and C. psittaci was studied in OF 1 and inbred C 57 Bl mice, inoculated intravenously (IV) or intraperitoneally. Antibodies were detected by a micro-immunofluorescence (MIF) test. Antibody kinetics depends on many factors (genetic particularities of the mice, route of inoculation, chlamydial strain). The response is more specific when mice are inoculated with C. trachomatis than with C. psittaci; heterospecific antibodies appear later in C 57 Bl mice than in OF 1 mice inoculated IV with C. trachomatis. These results are used to explain the serological findings in 6 cases of human chlamydial infections. The type, species and genus specificities of antibody responses to chlamydial infections are discussed.

Animals↗