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At least 199 records · Page 11Linked to original sources
PubMed1979

C- and G-banding patterns and chromosomal morphology of some breeds of Australian cattle.

A cytogenetical study using metaphase chromosomes from cultured lymphocytes, was made of 2 Banteng (Bibos banteng) steers and 218 bulls representing 13 purebreeds (Bos taurus type, Bos indicus type and Sanga) and 7 cross-breeds. Studies were made of photographic karyotypes of Giemsa stained and C-banded chromosomes of bulls of each breed and of B-banded chromosomes from 3 breeds of Bos indicus and one cross-breed Australian Friesian Sahiwal) cattle. The relative lengths of chromosomes of Bos taurus and Bos indicus bulls were compared and significant difference in relative lengths of the X chromosomes were noted between these two species. There was a differences in morphology of the Y chromosomes; Sanga, Banteng and Bos taurus type breeds had a small submetacentric Y chromosome, except for the Jersey which had a metacentric Y chromosome. All Bos indicus type bulls had an acrocentric Y chromosome but the Droughtmaster breed had two forms of the Y chromosome (submetacentric and acrocentric). The C-banding patterns of the autosomes and X chromosomes were similar for all breeds while those of the Y chromosomes of Bos indicus type cattle allowed their accurate identification. G-banding patterns of Bos indicus resembled those of Bos taurus and enabled pairing of homologous chromosomes. Centromeres of the autosomes were unstained but those of the sex chromosomes were darkly stained.

Animals↗
PubMed1984

Detection of Chlamydia trachomatis inclusions in McCoy and HeLa-229 cells: an alternative staining technique using toluidine blue.

Toluidine blue staining was used to detect Chlamydia trachomatis inclusions in both McCoy and HeLa-229 cells from clinical specimens. This method was more sensitive than iodine staining for detecting C trachomatis inclusions in both McCoy and HeLa-229 cells and also more sensitive than Giemsa staining for detecting chlamydial inclusions in HeLa-229 cells. While its sensitivity for detection of chlamydial inclusions in McCoy cells is equal to that of Giemsa staining, toluidine blue staining is easier and faster to perform.

Azure Stains↗
PubMed1979

On the nature of Romanowsky dyes and the Romanowsky-Giemsa effect.

This paper reviews the nature of Romanowsky staining and the relationship between Romanowsky dyes and the Romanowsky-Giemsa effect (RGE). On blood and bone marrow smears the RGE is characterized by a purple colouration of nuclei and neutrophil granules. The nuclear purple contrasts strongly with the blue cytoplasmic staining of cells rich in RNA. Requirement for the occurrence of RGE are: I A cationic dye: The best dye is azure B and, though azure A gives the nuclear purple colour, the cytoplasmic blue is inferior. No other cationic dye such as methylene blue is suitable. 2 An anionic dye: Most commonly eosin Y is used, but it can be replaced by the erythrosins. Full halogenation of the fluorescein (four atoms of bromine or iodine) is not necessary. Phloxine and rose bengal are unsuitable. 3 An appropriate substrate: These are proteins with acidic side groups or proteins bound to a polyanion. For the interaction with the dyes substrates must provide a suitable three-dimensional network which is why the RGE is not obtained in solutions. A tentative theory of RGE is advanced and briefly discussed.

Azure Stains↗
PubMed1986

Variation of differential cell counts of bronchoalveolar lavage fluid.

Bronchoalveolar lavage (BAL) is a common research and clinical tool to retrieve cells from the lower respiratory tract. Differential cell counts of the nucleated cells retrieved by BAL provide important diagnostic and prognostic information. There is much variation between laboratories in the reported normal percentages of lymphocytes and macrophages of cells retrieved by BAL. We compared three methods of identifying cells in the BAL fluid: a modified Wright-Giemsa (WG) stain, a nonspecific esterase stain, and a monoclonal marker for T lymphocytes. There was good agreement between the percentage of macrophages identified by WG and nonspecific esterase and the percentage of lymphocytes determined by WG stain and monoclonal marker. Intrasubject and intersubject agreement of the differential cell counts determined by the WG stain was good. We concluded that cells from BAL fluid can be analyzed using the WG stain alone.

Adolescent↗