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Distribution of PrP(Sc) in cattle with bovine spongiform encephalopathy slaughtered at abattoirs in Japan.

Three 80- to 95-month-old Holstein dairy cattle infected naturally with the agent of bovine spongiform encephalopathy (BSE) and slaughtered at abattoirs in Japan were examined for the distribution of disease-specific and protease-resistant prion protein (PrP(Sc)) by immunohistochemistry (IHC) and Western blot (WB) analyses. The cattle showed no clinical signs or symptoms relevant to BSE but were screened as positive by enzyme-linked immunosorbent assay, a rapid test for BSE. This positive result was confirmed by IHC or WB in a specimen of the medulla oblongata. Histopathologically, these cattle showed no vacuolation in tissue sections from the central nervous system except for the medulla oblongata. Both IHC and WB analyses revealed PrP(Sc) accumulation in the brain, spinal cord, satellite and ganglionic cells of the dorsal root ganglia, and the myenteric plexus of the distal ileum. In addition, small amounts of PrP(Sc) were detected in the peripheral nerves of 2 cattle by WB. No PrP(Sc) was demonstrated by either method in the Peyer's patches of the distal ileum; lymphoid tissues including the palatine tonsils, lymph nodes, and spleen; or other tissues. The distribution of PrP(Sc) accumulation in the preclinical stage was different between naturally infected cattle and cattle inoculated experimentally with the BSE agent.

Abattoirs↗

Condemnation of lungs in abattoirs in peninsular Malaysia due to parasitic infection from 1998-2004.

A study on causes of lung condemnation in 25 abattoirs from peninsular Malaysia for a period of seven years (1998-2004) was conducted by examining the records at the Department of Veterinary Services headquarters in Kuala Lumpur. A total of 5.3% of lungs from 233,417 cattle and buffaloes were condemned from 1998 to 2004. The main cause of condemnation was congestion (2.98%). The percentage of lungs that were condemned due to parasitic infection among the total population slaughtered was low (0.11%). Parasitic infection contributed to 2.1% of all lungs condemned. It was also found that the prevalence of parasitic infection in the lungs was generally much higher in buffaloes than in cattle.

Abattoirs↗

Anti-Helicobacter pylori IgG levels in abattoir workers.

The aim of our study was to detect antibodies against Helicobacter pylori (H. pylori) in abattoir workers whose activity was variable (pig, poultry-carcasses, poultry-intestinal organs treatment). An adsorption of all sera with H. pylori and Campylobacter species known to be ubiquitous and responsible for zoonosis was included in the ELISA reaction to eliminate possible cross-reactions between these species. Statistically higher specific titres of IgG antibodies against H. pylori were observed in men and women who had only worked for 1 or 2 years versus matched controls, indicating a possible transmission of H. pylori from animal to man. The role of poultry or pigs as contaminants alone or in association remains to be determined.

Abattoirs↗

Some myiasis producers in Cairo and Giza abattoirs.

The most important insects from medical point of view, are the blood suckers or the insect-borne diseases. Still others, almost non-blood suckers, but may attack man and animal to deposit their eggs or larvae causing pathogenic condition known as myiasis. In the present paper, a total of nine myiasis producing dipterous flies were collected from Cairo and Giza abattoirs. They were: Musca d. vicina, Calliphora vicina, Chrysomyia albiceps, C. marginalis, Lucilia sericata, L. cuprina, Sarcophaga haemorrhoidalis, Wohlfahrtia magnifica and Oestrus ovis. Their role as myiasis producers in man and animal was discussed.

Abattoirs↗

The prevalence of the larval stage of Taenia ovis at the Port Elizabeth Abattoir.

The prevalence of the larval stage of Taenia ovis and the origin of the animals were recorded for a period of 39 days at the Port Elizabeth abattoir. Cysticercosis was more frequently found in the M. triceps brachii of sheep, but the actual cysticerci were fewer than those found in the semimembranosus, semitendinosus, adductor, biceps femoris, vastus, rectus femoris and gracilis group of muscles of the hind limb (leg muscles). Most animals originated from farms in the Oudtshoorn district.

Abattoirs↗

[A toxicological hygiene study of a dye made from abattoir blood for sausage products].

Toxicologic and hygienic study of a pigment from abattoir blood--carboxyn (based on carboxyhemoglobin) was conducted on noninbred white rats in subacute (3 months) and chronic (12 months) experiments. The condition of the animals, their bw growth, blood morphology, erythrocyte sedimentation rate, the content of hemoglobin, protein and iron in the blood serum were evaluated. Activity of the following enzymes was studied: catalase and sorbitol dehydrogenase--in the blood serum; glucose 6-phosphate dehydrogenase, lactate dehydrogenase and cytochrome P-450 content--in the liver. Internal organs of the rats were subjected to histological investigation. The data obtained have evidenced the absence of some signs of biotransformation of the red complex of carboxyhemoglobin, or its toxic effect on the experimental animals. It has been concluded that carboxyn can be used as a coloring component in the production of sausage (in the amount of up to 2% of the sausage meat mass).

Abattoirs↗

Chemical composition and vitamin B content of abattoir by-product meals.

Chemical composition of meat-and-bone meals (n = 22), poultry by-product meals (n = 12), blood meals (n = 6), bone meals (n = 4) and feather meals (n = 8) indicated a slight degree of overdrying. Crude fat and ash contents were within the acceptable limits. Mineral elements of bones were determinative in the element pattern of meat-and-bone meals as indicated by the significant correlations between Ca, P and Mg. Crude protein was in negative regression and correlation with crude ash content (r = -0.81; P less than 0.001). This was more pronounced when the data were expressed in fatless dry matter (r = -0.94; P less than 0.001). Crude protein also showed significant negative correlations with Ca, P and Mg contents, offering the possibility to predict their levels from nitrogen content. Meat-and-bone meals and poultry by-product meals were relatively rich sources of riboflavin, niacin, pantothenic acid and biotin, whereas their pyridoxine and thiamine contents were low. Blood meals, bone meals and feather meals were found to be negligible vitamin B sources. Vitamin B and crude protein content of abattoir by-product meals showed uncertain correlations.

Abattoirs↗

[Serologic survey of brucellosis in the personnel of an abattoir in San Luis, Argentina].

Blood samples obtained from 256 workers of an abattoir were tested for brucellosis antibodies and 11.7% were found reactive. The highest rate was 44.2% among those who had contact with blood and viscera, and 26.1% among workers at slaughter section. Four tests were used: plaque agglutination (PA), tube agglutination (TA), card test (CT) and complement fixation (CF). The highest number of positive reactions was obtained by the PA and TA tests, with a complete coincidence in both results. When both test titers were the same or less than 50 IU, CF and CT yielded less percentage of positive results. When titers were higher than 50 IU, CT but CF showed concordance between the results.

Abattoirs↗

Streptococcus suis in slaughter pigs and abattoir workers.

The detection and identification of Streptococcus suis type 2 in 8.1% of 347 pig herds of southwestern Ontario revealed that the infection is widespread in this area. A herd suspected to be infected showed a carrier rate of 9.7% among the 62 animals sampled. These subclinical carriers represent a potential source of infection for slaughterhouse workers. From studies of contamination of hands and knives, it was concluded that eviscerators involved in removing the larynx and lungs from the carcasses have a significantly higher (p less than or equal to 0.05) risk of exposure to Streptococcus suis than other abattoir workers.

Abattoirs↗

The role of equipment that has direct contact with the carcase in the spread of Salmonella in a beef abattoir.

Counts of Salmonella were performed by the most probable number technique on 16 articles of abattoir equipment having direct contact with the carcase. Forty samples were collected from each article over 21 months. The contamination rate of these articles with salmonellae varied from nil % for a saw used to quarter the sides to 47.5% for stainless steel tables and hooks. Mesh gloves were also highly contaminated, salmonellae being isolated from 32.5% of gloves on the slaughter floor and 40% of those in the boning room. Salmonella counts ranged from 1.0 to 3,663 organisms per article. Mesh gloves, cutting boards and stainless steel tables were found to have counts that were at times greater than 1,000 salmonellae per article.

Abattoirs↗

Isolation of Yersinia enterocolitica from swine at an abattoir in Sweden.

Yersinia enterocolitica is presented from a taxonomic point of view. A review about the increased frequencies of isolations of Y. enterocolitica from domestic and wild animals in Europe is given. Recent studies from different countries strongly suggest swine as a source of Y. enterocolitica infection to humans. The frequencies of isolation of the O-group I (serotype 3) of this bacteria from swine at a Swedish abattoir is presented. The occurrence of positive cases in lots of swine sent to slaughter from different herds range from 0.6 to 8.0%.

Abattoirs↗

A brucellosis serological survey on beef cattle slaughtered at Cato Ridge Abattoir.

A total of 5059 sera were collected from adult female beef cattle at Cato Ridge Abattoir and tested for Brucella abortus antibody levels over the period November, 1981 to August, 1982. The sera were screened using the Rose Bengal Plate Test, and the Complement Fixation Test was used as the definitive test. Seventy-seven sera (1,5%) had titres of 30 IU complement fixing B. abortus antibody per ml serum or greater.

Abattoirs↗

Bovine parafilariasis: condemnations at the Cato Ridge abattoir.

Evidence presented shows a 585% increase in the compounded total carcase condemnations for parafilariasis at the Cato Ridge abattoir in the year July 1981/June 1982 as compared with July 1979/June 1980. There is also evidence which points to condemnations occurring in bi-annual cycles with peaks in November/December and June.

Abattoirs↗

Sarcocystosis in cattle and sheep at Prague abattoir.

A total of 288 animal oesophagi, including 154 from cattle and 134 from sheep of various age, were examined for the presence of sarcosporidians at Prague abattoir. The material from cattle was positive in 129 cases (84%) and all the three species of sarcosporidians hitherto described from cattle were identified: Sarcocystis cruzi, S. hirsuta and S. hominis. Among the sheep, 110 animals (82%) were infected. The macroscopic species S. tenella was found in 23 cases. The remaining positive findings were cystozoites and microscopic cysts of Sarcocystis including S. ovicanis (in 19 cases).

Abattoirs↗

The efficacy of bacteriological procedures for the isolation of Brucella abortus from abattoir material.

A process of emulsifying and centrifuging abattoir specimens before plating out is described. Brucella abortus was isolated more successfully by this process than by conventional methods, especially in low grade infections. The 5 different media used were equally effective in our attempts at isolation, but growth did not necessarily occur on all 5 plates. In dairy cows, specimens from supra-mammary lymph nodes, udder and iliac lymph nodes accounted for a high percentage of positive isolations.

Abattoirs↗

Outbreak of brucellosis at a South-Australian abattoir. 1. Clinical and serological findings.

During the period October 1979 to May 1980, 22 cases of acute brucellosis occurred at a South Australian abattoir. We obtained blood samples for serological investigations and culture of Brucella abortus, and tested the sera using the standard agglutination test (SAT), dithiothreitol test (DTT), anti-human globulin test (AHG) and complement fixation test (CFT). Patients showed large variations in antibody levels in each of these tests, both on presentation and after treatment. Blood culture was successful in six of 15 patients in whom it was attempted. Using the experience gained during the outbreak, we defined a set of serological criteria for the diagnosis of acute brucellosis, particularly in those who are occupationally exposed.

Abattoirs↗

A survey of salmonellae in trade cattle slaughtered at Nsukka abattoir.

A survey was carried out to determine the occurrence and carrier rate of Salmonella in trade cattle slaughtered for human consumption at Nsukka abattoir in Anambra State. Two hundred Zebu cattle were screened. A total of 424 specimens comprising of gall bladders, messenteric lymph nodes and rectal faeces were subjected to enterobacteriological studies. Identification of isolants was based on biochemical reactions and the use of polyvalent and group-specific antisera. Two Salmonella serotypes made up of Sal. typhimurium (3 strains) and Sal. dublin (5 strains) were obtained from 6 of the 200 cattle. A carrier rate of 3% was recorded. Two animals were carriers of Sal. typhimurium, while 4 cattle harboured Sal. dublin. Two strains of Sal. typhimurium and 3 of Sal. dublin were isolated from the gall bladder. The public health significance of the occurrence of anthropozoonotic Salmonellae in food animals as well as the hazard of consumption of bile are discussed.

Abattoirs↗

Retrospective diagnosis of Q fever ina country abattoir by the use of specific IgM globulin estimations.

Twenty-two cases of pyrexial illness which occurred amongst workers in a country abattoir were investigated retrospectively for Q fever, brucellosis, and leptospirosis. In 18, the illness was shown to be Q fever. No diagnoses were established for the other four. The demonstration of circulating Q-fever-specific IgM globulin was instrumental in establishing the diagnosis in many of the cases.

Abattoirs↗