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At least 181 records · Page 10Linked to original sources
PubMed1981

Treatment of acute left heart failure using dobutamine and intraaortic counterpulsation. Animal experiments and first clinical experiences.

Ten anesthetized mongrel dogs had a left anterolateral thoracotomy; the left anterior descending coronary artery was then ligated. After 60 min five animals each were treated either with dobutamine (4 microgram/min/kg; for 10 min), or with dobutamine and intraaortic counterpulsation. Combined treatment of cardiogenic shock proved superior. Those five dogs had significantly lower heart rates and dp/dt/p-values. Due to IABP the non-ischemic parts of the left ventricle were better perfused; there was no difference in treatment with regard to ischemic parts. The combined treatment was successfully inaugurated in two patients with cardiogenic shock.

Adult↗
PubMed1985

Aminoglycoside resistance in gram-negative bacilli during increased amikacin use. Comparison of experience in 14 United States hospitals with experience in the Minneapolis Veterans Administration Medical Center.

Resistance to amikacin, gentamicin, and tobramycin was surveyed prospectively during controlled aminoglycoside usage in 14 hospitals. Following an initial baseline period (minimum, three months) during which gentamicin use predominated, gentamicin and tobramycin were placed on restrictive control, establishing amikacin as the aminoglycoside of general use (86 percent of total aminoglycoside usage). During an average of 35 months' restriction of gentamicin and tobramycin, significant reductions in gram-negative resistance to gentamicin (8.4 to 7.0 percent, p less than 0.001) and tobramycin (6.0 to 5.3 percent, p less than 0.01) were observed. The most significant decreases in resistance to gentamicin and tobramycin (p less than 0.001) were found for Pseudomonas aeruginosa, Klebsiella species, Serratia species, and Proteus species. Amikacin resistance among gram-negative bacilli was observed to increase marginally from 1.4 to 1.7 percent (p less than 0.05) during the surveillance period, primarily due to a significant increase in resistance among P. aeruginosa (3.0 to 3.9 percent, p = 0.05). These data were compared with data from a similar surveillance program at the 700-bed Minneapolis Veterans Administration Medical Center. Over a period of 54 months, both gentamicin and tobramycin resistance decreased significantly when amikacin was used (p less than 0.001), then increased with reintroduction of gentamicin (p less than 0.05), and decreased significantly with reintroduction of amikacin (p less than 0.001). Despite predominant amikacin use for a total of 38 months, amikacin resistance did not increase and actually decreased significantly (p less than 0.05) in the last 12 months.

Amikacin↗
PubMed1994

Four-year experience with the CarboMedics valve: the North American experience. North American team of clinical investigators for the CarboMedics prosthetic heart valve.

A multicenter prospective trial of the CarboMedics valve was initiated in July 1988. Twelve North American centers were included for the purpose of establishing the safety and efficacy of this new rotatable, bileaflet, pyrolytic-Carbon prosthesis. The study included 786 patients (using 891 valves) enrolled between July 1988 and August 1992. There were 447 male and 339 female patients from ages 2 to 83 years. Of the 618 primary replacement operations (including double valves), 393 were aortic and 292 were mitral valve replacements. Mean follow-up time was 16.1 months; 94.7% of patients had completed documentation up to and including one year. New York Heart Association functional class was III or IV for 84.4% of patients preoperatively, and I and II in over 96% of patients for the first three postoperative years. Survival for the entire group was 93% (622) at 1 month, 89% (504) at 1 year, 87% (213) at 2 years, and 85% (41) at 3 years. There were no significant survival differences between primary and repeat replacements or between aortic, mitral, and double-valve replacements. Primary replacement patients with concomitant procedures (n = 241) had slightly decreased survival rates compared with those who underwent isolated procedures (isolated survival rate, 92% [98]; concomitant procedure survival rate, 81% [59] at 2 years; p = 0.002). There was no valve failure. Linearized rates (events per 100 patient-years) in primary replacement patients after 30 days included the following: thrombosis, 0.39; thromboembolism, 0.92; hemolysis, 0.53; anticoagulant-related hemorrhage, 2.36; paravalvular leak, 0.92; endocarditis, 0.53; reoperation, 0.92; and explantation, 0.66.(ABSTRACT TRUNCATED AT 250 WORDS)

Actuarial Analysis↗
PubMed1984

Liquid-holding experiments with human peripheral lymphocytes. II. Experiments with trenimon and 1-beta-D-arabinosylcytosine.

Liquid holding of trenimon-treated human peripheral lymphocytes in the G0 stage of the cell cycle leads to an elevation in the frequencies of chromosome aberrations and a decrease in the frequencies of chromatid aberrations. The frequencies of aberrant metaphases are not influenced significantly under these experimental conditions. Storage of trenimon-treated cells in the presence of 1-beta-D-arabinosylcytosine (araC) leads to an additional increase in the frequencies of chromosome-type aberrations, with an increase in the frequencies of aberrant metaphases as well. These findings are interpreted as DNA double-strand breaks being formed during the repair of damaged DNA, and that araC exaggerates this effect by inhibiting repair. AraC does not influence the frequencies of SCEs significantly, which indicates that either the lesions or the repair pathways leading to chromosome aberrations are different from those leading to SCEs.

Cells, Cultured↗
PubMed1977

Ileal substitute of ureter with reflux-plasty by terminal intussusception of bowel: animal experiments and clinical experience.

The conflicting results reported after substitution of the ureter by isolated bowel segments suggest that the procedure is still hazardous. This induced us to check experimentally the performance of the ileal ureter with antireflux-plasty before using it clinically. The antireflux mechanism is constructed by intussuscepting the terminal 8 cm. of an isolated ileal segment into each other thus forming a nipple. After vesicoileostomy the nipple protrudes into the urinary bladder. In the pig vesicoileorenal reflux was prevented, and anterograde urinary flow from the kidney through the ileal ureter into the bladder was unobstructed. Finally, the case of a patient is recorded who was submitted to the same procedure successfully.

Animals↗
PubMed1992

The cold chain from manufacturer to vaccinator: experiments and experiences.

Biological substances are temperature sensitive. Most vaccines must be constantly kept at temperatures between +2 to +8 degrees C (cold chain). To test the conditions of a long distance transport of vaccines in an 80 l cool box the temperature in the correctly packed box was monitored. The temperature remained between zero and +8 degrees C for more than 90 h, the ambient temperature during this test reached 37 degrees C for 5 h. It can be concluded that the currently used transport boxes are well suited for safe transport of sensitive products such as vaccines.

Cryopreservation↗
PubMed1982

The relationship between the activity of splenic enzymes and the immune response in the goldfish, Carassius auratus. I. Preliminary experiments with human A1 secretor's saliva and A1 RBC. II. Preliminary enzyme experiments using alpha-GPD and LDH.

1. Goldfish, Carassius auratus, were injected with two antigenic substances A1 secretor's saliva or A1 RBC. 2. The goldfish produced significant antibody titers which continued for 10-12 weeks when maintained at 28 degrees C. 3. Analysis of enzymatic activities of soluble and particulate alpha-GPD and LDH from skeletal muscle and spleen showed significant differences between and within tissues.

ABO Blood-Group System↗
PubMed1994

Cologne Burn Centre experiences with glycerol-preserved allogeneic skin: Part I: Clinical experiences and histological findings (overgraft and sandwich technique).

In an effort to improve the take of finely meshed autografts a modification of the sandwich technique, as first published by Alexander et al. (1981), was developed. In contrast to the techniques described by other authors, the wound bed is sealed with fibrin glue spray after excision of the burns. Widely meshed autografts are then covered with non-meshed (only scarcely sliced) glycerolized allograft sheets, being fixed with staples. Patients are placed on fluidized beds and are exposed without dressings from the fifth day onwards. Histologically and clinically, it can be assumed that part of the glycerolized allodermis is incorporated. During the weeks after transplantation, a creeping substitution of the allodermis by autologous tissue takes place. This would suggest a co-existence between glycerol-preserved hypo-allergenic allografts and auto-epidermis. Research on the definitive fate of allodermis in cases of sandwich grafting is continuing.

Adult↗