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Interface interaction controlled transport of CdTe nanoparticles in the microcontact printing process.

High-quality CdTe nanoparticles stabilized with thioglycolic acid (TGA) are patterned on SiO2/Si surfaces using microcontact printing (microCP). Due to the weak interaction of the nanoparticles with the stamp surface, tailoring of gas flow rate during the inking process as well as the type and scale of the patterns on the stamp are used to control the distribution of the nanoparticles on the structured stamp surface. This distribution is then transferred the printed regions. Either edge printing or homogeneous printing can be achieved under optimized conditions. In addition, new structures such as nanowires form under certain conditions.

Journal Article↗

A comparative study of three techniques for the analysis of sperm recovery: touch-print cytology, wet preparation, and testicular histopathology.

PURPOSE: The aim of this study is to evaluate the efficacy of simultaneous testicular touch-print cytology, testicular histopathology, and wet preparation in nonobstructive azoospermic (NOA) males. METHODS: Three hundred and sixty-three males with NOA underwent a multiple testicular sampling prior to ICSI for histopathologic evaluation, diagnostic testicular sperm extraction, and simultaneous touch-print cytology to evaluate sperm presence or absence. A total of 979 testicular samples were taken. RESULTS: Sperm recovery was achieved in 106 cases (29.2%). Patients with hypospermatogenesis and focal spermatogenesis needed 2.8 and 5.9 biopsies, respectively, to retrieve spermatozoa, while in patients with germ cell aplasia and maturation arrest, even after eight to nine samples no spermatozoa were recovered. Neither the FSH levels nor the testicular volume was found to be significant in the prediction of sperm recovery. If only a single testis was to be biopsied, 25% of the cases with sperm recovery would have been missed. The combination of touch-print cytology with histopathology and wet preparation increased the accuracy of spermatozoa identification. CONCLUSION: Touch-print cytology was found to be more predictive than wet preparation in the diagnosis of spermatogenesis; moreover, it was found to be a quick and easy technique providing an accurate diagnosis in prediction of sperm recovery.

Adult↗

Phonetic recoding of phonologically ambiguous printed words.

Speech detection and matching simultaneously presented printed and spoken words were used to examine phonologic and phonetic processing of Hebrew heterophonic homographs. Subjects detected a correspondence between an ambiguous letter string and the amplitude envelopes of both dominant and subordinate phonological alternatives. Similar effects were obtained when the homographs were phonologically disambiguated by adding vowel marks. The matching of the unpointed printed forms of heterophonic homographs to the dominant and subordinate spoken alternatives presented auditorily was as fast as matching the pointed unambiguous forms to the respective spoken words. This outcome was not obtained when print and speech were not presented simultaneously. These results suggest that printed heterophonic homographs activate the two spoken alternatives they represent and provide further confirmation for fast phonetic recoding in reading.

Acoustic Stimulation↗

Interpersonal and print nutrition communication for a Spanish-dominant Latino population: Secretos de la Buena Vida.

Participants (N=357) were randomly assigned to 1 of 3 conditions: lay health advisor (promotora) plus tailored print materials, tailored print materials only (tailored), or off-the-shelf print materials (control). The primary outcomes were calories from fat and daily grams of fiber. Secondary outcomes included total energy intake, total and saturated fat intake, and total carbohydrates. Adjusted for baseline values, calories from fat were 29%, 30%, and 30% for the promotora, tailored, and control conditions, respectively, and grams of fiber consumed were 16 g, 17 g, and 16 g. Significant Condition X Time interactions were not observed between baseline and 12-weeks postintervention. The LHA condition achieved significantly lower levels of energy intake, total fat and saturated fat, and total carbohydrates. The relative superiority of the promotora condition may derive from the personal touch achieved in the face-to-face interactions or from the women's use of print materials under the promotora's guidance.

Acculturation↗

The development of substitute inks and controls for reducing workplace concentrations of organic solvent vapors in a vinyl shower curtain printing plant.

During the summer of 1994, football players at a practice field reported noxious odors in the area. Ohio Environmental Protection Agency (OEPA) investigations of industries surrounding the field included a printing facility producing vinyl shower curtains with screen-printed designs. Though not the source of the odor, they were discharging volatile organic compounds directly to the environs in violation of OEPA regulations. To achieve compliance they installed a catalytic oxidizer for treating discharged air. Due to high equipment costs, the capacity of the installed catalytic oxidizer resulted in a substantial reduction in discharged air flow rates and increased solvent vapor concentrations within the workplace. Vapor levels caused worker discomfort, prompting a request for assistance from the Ohio Bureau of Workers Compensation. The vapor concentrations were found to exceed NIOSH, OSHA, and ACGIH acceptable exposure levels. The workers were then required to wear organic vapor removing respirators full-time while printing as a temporary protective measure. The company requested NIOSH assistance in finding methods to reduce solvent vapor concentrations. NIOSH studies included the identification of the sources and relative magnitude of solvent emissions from the printing process, the design of controls for the emissions, and the development of substitute inks using non-photochemically reactive solvents. The new ink system and controls allowed OEPA removal of the requirement for the treatment of discharged air and substantial increases in dilution ventilation. Increased ventilation would permit reduction in worker exposures to less than 1/3 mixture TLV levels and removal of requirements for respirator usage. This solution was the result of a comprehensive review of all facets of the problem, including OEPA regulations. It also required cooperative work between the company and federal, state, and local governmental agencies.

Air Movements↗

Laser printing of pluripotent embryonal carcinoma cells.

A technique by which to print patterns and multilayers of scaffolding and living cells could be used in tissue engineering to fabricate tissue constructs with cells, materials, and chemical diversity at the micron scale. We describe here studies using a laser forward transfer technology to print single-layer patterns of pluripotent murine embryonal carcinoma cells. This report focuses on verifying cell viability and functionality as well as the ability to differentiate cells after laser transfer. We find that when cells are printed onto model tissue scaffolding such as a layer of hydrogel, greater than 95% of the cells survive the transfer process and remain viable. In addition, alkaline comet assays were performed on transferred cells, showing minimal single-strand DNA damage from potential ultraviolet-cell interaction. We also find that laser-transferred cells express microtubular associated protein 2 after retinoic acid stimulus and myosin heavy chain protein after dimethyl sulfoxide stimulus, indicating successful neural and muscular pathway differentiation. These studies provide a foundation so that laser printing may next be used to build heterogeneous multilayer cellular structures, enabling cell growth and differentiation in heterogeneous three-dimensional environments to be uniquely studied.

Animals↗

The evaluation of exposure of printing trade employees to polycyclic aromatic hydrocarbons.

Epidemiological studies of the lung cancer experience of workers in the printing industry have been inconclusive. Where there have been positive findings, the effect has generally been attributed to polycyclic aromatic hydrocarbon (PAH) exposure, although no exposure measurements were made. This study was undertaken to determine the exposures of printing press operators to PAH and total particulate (TP), and to evaluate the factors contributing to exposure. Personal time-weighted average exposures of press room workers were determined on two consecutive days at nine sites, including two newspaper operations. The average PAH exposure was 16.5 micrograms m-3 (including naphthalene). The average TP exposure was 0.63 mg m-3. Examination of the data revealed that there were significant differences between sites for exposure both to TP and to PAH. Newspaper plants had significantly lower exposures than commercial printing operations. There were no significant differences in exposure between the various job classifications of workers in the press rooms. Factors identified as contributing to exposure to TP were: the effectiveness of the ventilation systems, the method of feeding the press, the type of paper and the print impression area.

Aerosols↗

Eye movements and reading with large print and optical magnifiers in macular disease.

PURPOSE: Reading rate has been the main performance measure in studies that have compared reading with large print and optical magnifiers; eye movement characteristics have not been considered. We compared both eye movement characteristics and reading rates for subjects with macular disease reading without and with a range of low-vision devices. METHODS: Silent reading rate and eye movement characteristics for text passages at critical print size of 21 subjects aged 14 to 88 years with macular disease were measured with and without their preferred low-vision device. Saccadic frequency was determined from a sequencing task comprising five letters each separated by 5 degrees. Eye movements were recorded using an infrared limbal reflection system. RESULTS: There were no significant differences in reading rate, fixation durations, saccade numbers per word, or percent retrace time when using a low-vision device compared with reading without a low-vision device. The percentage of regressions was, however, lower with the low-vision device. Saccadic frequency in the sequencing task was predictive of reading performance with and without a low-vision device. CONCLUSIONS: When reading at critical print size, in terms of reading rate or saccades per word, there was no advantage to using large print over an optical low-vision device.

Adolescent↗

Tissue Printing as a Tool for Observing Immunological and Protein Profiles in Young and Mature Celery Petioles.

Tissue printing onto membranes such as nitrocellulose is a technique employed to study the localization of proteins, nucleic acids, and soluble metabolites from freshly cut tissue slices. We probed tissue prints of young and mature celery (Apium graveolens) petioles with antibodies raised against two proteins, spinach ribulose-1,5-bisphosphate carboxylase and tomato fruit catalase. The purposes of this study were to determine if these proteins are developmentally regulated and to determine if the patterns and intensities of cross-reactivity of antibodies on tissue blots corresponded only to the presence of specific epitopes or was related to the amount of protein present in any given area on the tissue prints. Different and distinct cross-reactivity patterns were observed with each of the two antibodies used. Tissue prints from young and mature tissues also showed differences in antibody cross-reactivity. Comparison of Coomassie blue staining patterns with antibody reactivity patterns showed that there is little relationship between tissue protein concentration and antibody reactivity.

Journal Article↗

Machine printed text and handwriting identification in noisy document images.

In this paper, we address the problem of the identification of text in noisy document images. We are especially focused on segmenting and identifying between handwriting and machine printed text because: 1) Handwriting in a document often indicates corrections, additions, or other supplemental information that should be treated differently from the main content and 2) the segmentation and recognition techniques requested for machine printed and handwritten text are significantly different. A novel aspect of our approach is that we treat noise as a separate class and model noise based on selected features. Trained Fisher classifiers are used to identify machine printed text and handwriting from noise and we further exploit context to refine the classification. A Markov Random Field-based (MRF) approach is used to model the geometrical structure of the printed text, handwriting, and noise to rectify misclassifications. Experimental results show that our approach is robust and can significantly improve page segmentation in noisy document collections.

Algorithms↗

The palm print as a sensitive predictor of difficult laryngoscopy in diabetics.

BACKGROUND: Grading of the ink impression made by the palm of the hand has been proposed as a means of screening diabetic patients in whom tracheal intubation may be difficult because of glycosylation of both metacarpal and cervical joints. The current study was designed to test the predictability of the palm print and other airway evaluation indices in a diabetic population. METHOD: 83 adult, diabetic patients scheduled for surgery under general anesthesia were evaluated preoperatively using 4 physical examination indices predictive of difficult laryngoscopy and intubation: Mallampati classification, thyromental distance, head extension, and palm print. Following induction of anesthesia and neuromuscular relaxation, laryngoscopy was performed and the laryngoscopic view scored. The sensitivity, specificity and positive predictive value of each airway evaluation index was calculated. RESULTS: The palm print had the highest sensitivity of all indices (100%). The other 3 indices failed to detect 9 to 13 difficult laryngoscopies. CONCLUSION: We believe that in the diabetic population, the palm print index may be a sensitive marker of difficult intubation. It is possible that other airway evaluation indices would prove more sensitive, and have greater utility, in distinct populations as compared to when they are applied to all patients.

Adult↗

Printed patient education interventions to facilitate shared management of chronic disease: a literature review.

BACKGROUND: Printed materials for patient self-education are a comparatively low cost, flexible and potentially useful aid for dealing with the growing burden of chronic disease in our society. A comprehensive review of existing trials is required. AIMS: We conducted a comprehensive review which aimed to (i) evaluate the evidence for effectiveness of print-only interventions in increasing patient participation in chronic disease management and (ii) identify disease or intervention characteristics associated with success. METHODS: Medline, the Cochrane Central Register of Controlled Trials, EMBASE and CINAHL were searched for the period 1992 to January 2005 for reports of controlled trials of printed patient materials in chronic disease. The quality of both studies and interventions was assessed. RESULTS: Seven studies were found. They focussed on inflammatory bowel disease, arthritis, asthma and diabetes. Few studies named primary outcomes, continued beyond 6 months or included process evaluations. Few reports gave the behaviour-change rationale of the intervention. Few interventions were reported as having been designed using testing and improvement, psychosocial theory, checks of patient understanding or other patient input. A variety of outcomes was measured. Statistically significant change was found for few. Knowledge improved in three studies, adherence improved in two and quality of life declined in one. CONCLUSIONS: We found that printed interventions as currently designed appear to produce, at best, modest benefits. The design of interventions, studies and study reports all need improvement before decisions can be made about effectiveness and characteristics associated with success.

Arthritis↗

The American print news media 'construction' of five natural disasters.

In 1985, five international 'natural' disasters received prominent print news media coverage in the United States. Content analyses of selected print news media accounts of these five disasters were conducted. The purported evidence of alleged cause-effect relationships describing and explaining these disasters as 'objective' realities was evaluated in the light of the subjective selection of explanatory factors, themes, frameworks, and value assumptions which underlie the media's analysis and 'construction' of these events as 'natural' disasters. Analysis of the American print news media coverage of these disasters indicated an emphasis upon the dramatic, descriptive, climatological or geological qualities of these events rather than upon causal explanations emphasizing the role of human acts or omissions in the development of these disasters. The print news media 'constructed' these events as 'natural' disasters despite clear evidence of their hybrid, natural-human origins.

Bangladesh↗

Interpretation of digital mammograms: comparison of speed and accuracy of soft-copy versus printed-film display.

PURPOSE: To compare the speed and accuracy of the interpretations of digital mammograms by radiologists by using printed-film versus soft-copy display. MATERIALS AND METHODS: After being trained in interpretation of digital mammograms, eight radiologists interpreted 63 digital mammograms, all with old studies for comparison. All studies were interpreted by all readers in soft-copy and printed-film display, with interpretations of images in the same cases at least 1 month apart. Mammograms were interpreted in cases that included six biopsy-proved cancers and 20 biopsy-proved benign lesions, 20 cases of probably benign findings in patients who underwent 6-month follow-up, and 17 cases without apparent findings. Area under the receiver operating characteristic curve (A(z)), sensitivity, and specificity were calculated for soft-copy and printed-film display. RESULTS: There was no significant difference in the speed of interpretation, but interpretations with soft-copy display were slightly faster. The differences in A(z), sensitivity, and specificity were not significantly different; A(z) and sensitivity were slightly better for interpretations with printed film, and specificity was slightly better for interpretations with soft copy. CONCLUSION: Interpretation with soft-copy display is likely to be useful with digital mammography and is unlikely to significantly change accuracy or speed.

Breast Diseases↗

Effect of electronic versus print format and different reading resources on knowledge acquisition in the third-year medicine clerkship.

BACKGROUND: Many medical students have access to electronic and print resources. It is not known if the format or the choice of resources effects knowledge acquisition over an entire clerkship. PURPOSE: To determine if the format (electronic or print) or choice of reading materials is associated with knowledge acquisition during the 3-year internal medicine clerkship. METHODS: This was a prospective cohort study. During the last week of the clerkship, students took the National Board of Medical Examiners (NBME) exam and completed a survey ranking the reading materials used. RESULTS: One hundred and fourteen 3rd-year internal medicine clerkship students participated. The most commonly reported resources were UpToDate (99%), a review or question book (93%), and Harrison's Principles of Internal Medicine (82%). Multivariate analysis showed a significant beneficial effect of any use of Harrison's (linear regression, p < .04, absolute difference 3 points, SD = 1.4) on NBME score. The format of the most important resource-electronic (61%) or print (39%)-did not predict the NBME score, p > .80. CONCLUSIONS: The format (electronic or print) of resources did not appear to effect NBME score in the medicine clerkship. A secondary analysis showed the use of Harrison's textbook was associated with improved knowledge acquisition.

Adult↗

Spelling and emergent picture-printed word relations established with delayed identity matching to complex samples.

Students with academic deficits learned delayed matching-to-sample tasks that used complex sample stimuli, each consisting of a picture and a printed word. A touch to the sample complex removed it from the computer display and produced either picture comparisons or a choice pool of letters. If the comparisons were pictures, selecting the picture identical to the preceding sample was reinforced. If the letters appeared, letter-by-letter construction of the preceding printed word sample was reinforced. The procedure engendered new constructed-response spelling performances and arbitrary relations among pictures and printed words in matching to sample. The emergence of relations among different sets of printed words (paired with the same pictures) suggested classes of equivalent stimuli. Outcome tests involving spoken words as sample stimuli suggested expansion of subjects' spelling repertoires and stimulus classes.

Adolescent↗

Cryostat tissue printing: an improved method for histochemical and immunocytochemical localization in soft tissues.

A modification of a previously described tissue print technique has been developed in which soft tissues are frozen and sectioned in a cryostat prior to direct collection on nitrocellulose or nylon membranes. The inexpensive embedding technique described here allows accurate orientation of specimens prior to mounting, and mounted material may be stored easily after initial sectioning for future reexamination. Standard hand tissue prints of soft specimens exhibit tissue distortion and uneven delivery of material to the membrane, which limits resolution and makes interpretation difficult. Cryostat sections retain tissue fragments in their original arrangement relative to each other during printing and deliver a consistent and quantitative amount of material from all parts of the specimen. The cryostat tissue print technique is applied here to immature floral buds, demonstrating the tissue-level histochemical localization of beta-glucuronidase in transgenic plants and immunolocalization of a novel protein present only in mutant plants. This modified technique is applicable for examining both plant and animal tissues.

Cryoultramicrotomy↗

Tissue printing for peroxidases associated with lignification.

Peroxidases with syringaldazine oxidase activity have been claimed to be specifically involved in lignification. The use of syringaldazine as a substrate presents problems of color diffusion and fading. We report a method for timing color development of syringaldazine peroxidase reactions in tissue prints, and conditions under which diffusion is minimized. The best time interval in which the tissue print and background gave significant contrast was determined using ELISA plates in which impressions of the tissue could be made, mimicking reactions on a membrane. This technique permitted simultaneous reading of a large number of samples and blanks, rendering enough data points for statistical analysis. Optimum development time for syringaldazine peroxidase reactions was between 10 and 20 min. Color diffusion and smearing of syringaldazine peroxidase reactions in tissue prints were minimized when prints were covered with a strip of membrane before adding the substrate mixture.

Brassica↗