Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Optimum Population”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 181 records · Page 10Linked to original sources

Isolation of a spontaneous CHO amino acid transport mutant by a combination of tritium suicide and replica plating.

A spontaneous transport mutant of Chinese hamster ovary cells, CHY-1, was isolated by a combination of [3H]proline suicide and replica plating. The mutant took up less tritium than the parent, resulting in a lower killing rate during storage. Transport by four separate amino acid transport systems (A, ASC, L, Ly+) was examined. The CHY-1 mutant exhibited normal uptake via the ASC, L, and Ly+ systems. By contrast, uptake of the most specific substrate of the A system, 2-(methylamino)-isobutyric acid, was significantly reduced at low, but not high, concentrations, due to a 3.5-fold increase in Km and a 1.5-fold increase in Vmax. Taken together, these data suggest that the CHY-1 mutation may be in the structural gene coding for the A transport protein. The tritium suicide procedure is discussed, and general equations are derived to predict the maximum storage time for the survival of one mutant cell and the optimum size of the cell population for maximum mutant enrichment.

Amino Acids↗

alpha Interferon: the potential drug of adjuvant therapy: past achievements and future challenges.

This paper aims to summarize current experience with alpha interferon and provide direction for future study. There are four areas in which alpha interferon has proven or potential activity: antiviral, premalignant, adjuvant and advanced disease settings. The three main viral diseases in which interferon alfa-2b has been shown to have activity are chronic viral hepatitis, acquired immunodeficiency syndrome, and human papilloma virus infections. In vitro studies suggest that alpha interferon may inhibit transformation of some premalignant conditions into malignant disease; e.g., vaginal intraepithelial neoplasia. In the adjuvant setting, it is possible that a biological response modifier, such as alpha interferon, may have a role in helping the immune system to destroy residual tumour cells following tumour bulk reduction with radiation or chemotherapy. A higher response rate has been seen in patients with small tumour bulk compared to those with large tumour bulk (e.g., malignant melanoma, ovarian carcinoma), and in patients with early, rather than late, disease (e.g., chronic myelogenous leukaemia, hairy cell leukaemia, multiple myeloma, non-Hodgkin's lymphoma). This may be due to efficacy in a small tumour bulk setting or due to an immunoadjuvant role. In advanced disease, the question is how best to exploit the possible synergistic effects between alpha interferon and other therapeutic modalities. The optimum dose, schedule and patient populations for combined treatment have yet to be determined. The major objective of this paper is to determine how best to capitalize upon the current state of knowledge to build for future trials of alpha interferon, and to determine whether the existing data suggest an adjuvant role for interferon after initial tumour regression.

Combined Modality Therapy↗

Microbe inhibition by Tribolium flour beetles varies with beetle species, strain, sex, and microbe group.

Tribolium flour beetles produce defensive compounds, including quinones, putatively aimed at deterring predators and inhibiting microbes. Here we examine how effective the defensive secretions of Tribolium confusum and T. castaneum are at inhibiting growth of various microbes and how this varies with species, geographic strain, and sex of the beetles. We explore differences at both the kingdom and species level of common flour microbes in their susceptibility to defensive compounds. Beetle species and strains vary in their ability to inhibit microbial growth. In addition, microbes vary in their sensitivity to the beetles' defense compounds. The capability to suppress microbial growth is likely under stabilizing selection with optimum quinone production varying among populations and may be dependent on several environmental factors including temperature, humidity, and predators.

Animals↗

A fundamental metric for continuity of care: modeling and performance evaluation.

The concept of continuity in medicine is fundamental and it refers to the delivery of medical care to a patient by a care provider in an uninterrupted and coordinated manner and in accordance with the medical care needs of the patient. Principal limitations of previous studies include the following. First, there is the absence of a deliberate effort to schedule a patient's successive visits with the same provider. Second, in most cases, the reported measures are derived from the data collected through questionnaires that patients and doctors are asked to complete from memory. This introduces an unreliability factor. Third, the reported continuity-related measures are restricted to the available patient sample and, as a result, they do not necessarily reflect a representative patient distribution by age in a typical community. Fourth, the relationship between the patient-doctor interaction interval and the continuity of medical care has never been explored systematically in the literature. This paper models continuity for a representative staff model health maintenance organization (HMO) clinic in suburban Arizona and simulates patient visits that are stochastically generated through utilizing representative numbers of patient visits and care providers. It focuses on individual-based continuity delivered by a primary care provider, i.e., a general practitioner. In the simulation, the visit patterns of patients, their ages, and the length of the interaction episodes are synthesized stochastically. The distributions reflect both the patient visit profile, by age, inherent in the 90,000+ patient electronic records collected at the CIGNA HMO clinic at Chandler, AZ, over a three-year period, and the population distribution inherent in the CIGNA records. A key characteristic of the model is that it aims at deliberately maximizing continuity by making a strong effort to schedule both a patient's regular visit and follow-ups with the primary care provider, subject to the provider's availability and schedule. This paper proposes a new definition of continuity, fundamental continuity of care index (FCCI), and argues that, fundamentally, a primary care provider's depth of understanding of the patient is directly proportional to the total length of interaction between the patient and the primary care provider. Utilizing the CIGNA summary data, this paper organizes patients into five age groups: 0-5 years, 5-15 years, 15-45 years, 45-60 years, and 60+ and synthesizes the corresponding arrival distributions. Performance results, obtained for a total of 55,056 patient visits over a three-year period indicate that, while 94-97% of the patient visits are with the primary care provider, patients spend 76-77% of the nominal total visit time with their primary care provider, leading to FCCI values ranging from 0.72 to 0.75. Performance results indicate that for different choices of the mean of the patient-doctor interaction duration, the average FCCI values, obtained following simulation, reveal an approximate bell-shaped graph. For values of the mean ranging from 5 to 10, 15, 20, 25, 35, 45, and 55 min, the average FCCI measure starts at 0.49, increases to a maximum of 0.88 corresponding to a mean of 25 min, and then decreases to 0.60. Thus, for a medical clinic, constrained by the number of care providers servicing a fixed number of patients, for a given population distribution by age, an optimum value for the average patient-doctor interaction duration may be obtained empirically that yields the maximal continuity measure.

Arizona↗

Morphological, biochemical, and growth characteristics of pseudomonas cepacia from distilled water.

Studies were conducted on three strains of Pseudomonas cepacia isolated and maintained in distilled water and on a laboratory-subcultured strain transferred to distilled water. Optimum growth rates and maximum population yields of the four strains in distilled water were obtained at 37 C, although high population levels (10(6)-10(7)/ml) were reached and maintained over extended incubation periods at temperatures from 18 C to 42 C. Two strains were able to grow in distilled water at temperatures ranging from 12 C to 48 C and to survive 48 h and 21 days at 50 C and 10 C, respectively. Cells from distilled water cultures inoculated into Trypticase soy broth showed an immediate two- to three-log drop at upper and lower temperature limits; survivors were able to initiate logarithmic growth. Results obtained in morphological, biochemical, and antibiotic tests affirmed the strain differences noted in growth studies.

Aerobiosis↗

Acid reactivity of carbonated apatites with strontium and fluoride substitutions.

Carbonated apatites containing approximately 3.0 or 6.5% CO3 were precipitated in the presence of sodium, strontium, and/or fluoride at various concentrations. Almost all the available strontium and fluoride was incorporated into the crystals, whereas only a portion of the carbonate and sodium present was taken up. When incorporated together, Sr and F improved the crystallinity of carbonated apatites and markedly reduced their acid reactivity. This phenomenon may partially explain the observed reduction in caries for populations whose drinking water contains optimum levels of Sr at 5-10 mg/l and F at 1-2 mg/l.

Apatites↗

Children's health care: brief report. ECI: a study in health care coordination.

The delivery of appropriate services to handicapped and developmentally delayed young children is an increasingly complex undertaking. No single profession is responsible for providing these services; no single agency is responsible for their administration. In a concerted effort to coordinate these services and assure optimum benefit to the target population, an amalgam of political, professional, and parent forces in Texas led to the creation of ECI--the Early Childhood Intervention Program. This program and the way in which it has evolved exemplifies how disparate groups can work together to provide a comprehensive system of children's health care.

Child Health Services↗

Results of the ATLAS study. High or low doses of ACE inhibitors for heart failure?

In a large, randomized study, patients with heart failure who received a large daily dose of an angiotensin-converting inhibitor had a trend toward a lower mortality rate than did patients who received a low daily dose. Moreover, the hospitalization rate was lower in the high-dose group, and the side-effect profile was the same in both groups. Physicians should try to give maximal doses to achieve optimum benefit in this patient population.

Adult↗

Creating the continuum of care.

Continuum of care is a concept involving an integrated system of care that guides and tracks patient over time through a comprehensive array of health services spanning all levels of intensity of care. This article presents a definition of continuum of care, including objectives, basic components, and operating principles. The purpose is to provide a concept that will encourage leadership by offering a vision for the future and providing a framework to guide day-to-day decision-making. Two case scenarios illustrate the effectiveness of the continuum of care concept in providing a framework for delivery of optimum health care to patient populations.

Continuity of Patient Care↗

[The effect of cage type and population density on the body weight development of laboratory mice].

The effect of different keeping conditions on body weight gain was studied in 200 male Fzt:DU mice between days 21 and 70 of life. The animals were divided in four different housing groups: DU-B (housing in stress cages), DU-B+HI (housing in stress cages and afterwards in standard cages type HI), DU-HI (housing in standard cages type HI) and DU-S+AP (housing in standard group cages). Housing in stress cages resulted in lower body weight gain at every age; subsequent housing in standard cages type HI for one week failed to compensate this effects. Likewise, keeping of four mice in standard cages type I (DU-HI) between days 42 and 70 of life also resulted in delayed body weight gain. It is possible that the chosen population density was not an optimum for normal weight gain during the adult life. Animals kept in standard stocking cages (DU-S+AP) had the highest body weight gain.

Animals↗

[Relationship between sexual reproduction, population growth and resting egg production of freshwater rotifer Brachionus calyciflorus].

Using exponential and logistic growth models, the effects of sexual reproduction frequency and the proportion of a female's daughters reproducing sexually on population growth rate and resting egg production of freshwater rotifer Brachionus calyciflorus were examined by computer simulation. Within the parameters of the simulation, the percentage of micitc females in the offspring required for maximum resting egg production increased from 9% to 69%, when the frequency of sexual reproduction decreased from 100% to 20%. The increase of density-dependent limitation (the value of K decreased from 1000 to 100) made this percentage increase from 18% to 69%, and resting egg production decrease from 1072.10 to 133.67. The relationship between the population growth rate (r) and the optimum percentage of mictic females in the offspring required for maximum resting egg production was best described by curvilinear regression. When sexual reproduction became more frequent and density-dependent decreased, The population with the percentage of mictic females in the offspring in 10-30% had a bigger resting egg production.

Animals↗

Medical audit used for estimation of optimum level of outpatient care.

OBJECTIVE: To create a model for determining the optimum level of outpatient medical care and to determine this level. DESIGN: Expert committees were established comprising one or two hospital physicians and two general practitioners who checked medical records for all outpatient visits to doctors made by a defined population during a defined period of time. The determination of optimum level was made blindly by each member of the expert committee. SETTING AND PARTICIPANTS: The study comprised all visits to physicians during 10 weeks, in total more than 4,000, made by the people in a Swedish community, of which 2,084 were randomized. RESULTS: Total agreement between the members of the committee was initially reached for 84% of the visits and, after a common discussion between the committee members, for 99%. A general practitioner was considered to be the optimum level of care for 76% of the patients in the total series, in the upper ages (above 80) for about 85%. CONCLUSION: This method seemed suitable for determining the optimum level of care in a population and may be of value when planning for an optimum health care service. Based on the results from our study it seems reasonable to assume that general practitioners whose training corresponds to that of Swedish ones are competent to treat about 75% of all consultations.

Adolescent↗

Multiscale classification using nearest neighbor density estimates.

Density estimates based on k-nearest neighbors have useful applications in nonparametric discriminant analysis. In classification problems, optimal values of k are usually estimated by minimizing the cross-validated misclassification rates. However, these cross-validation techniques allow only one value of k for each population density estimate, while in a classification problem, the optimum value of k for a class may also depend on its competing population densities. Further, it is computationally difficult to minimize the cross-validated error rate when there are several competing populations. Moreover, in addition to depending on the entire training data set, a good choice of k should also depend on the specific observation to be classified. Therefore, instead of using a single value of k for each population density estimate, it is more useful in practice to consider the results for multiple values of k to arrive at the final decision. This paper presents one such approach along with a graphical device, which gives more information about classification results for various choices of k and the related statistical uncertainties present there. The utility of this proposed methodology has been illustrated using some benchmark data sets.

Algorithms↗

Epidemic diseases and host clustering: an optimum cluster size ensures maximum survival.

The risk of infection is generally believed to increase with gregariousness. Using a discrete time spatial simulation model we tested the effect of host clustering on transmission of infection within a population. Over a large parameter range, an optimum cluster size was observed to ensure maximum probability of escaping infection. Although the within cluster transmission increased with cluster size, the between cluster distance increased thereby decreasing the probability of transmission across clusters. The probability of stochastic extinction of the pathogen also increased with increasing cluster size. Thus contrary to the popular belief, clustering can be an effective strategy to minimize the risk of infections.

Computer Simulation↗

Competition between phenotypes.

We present two models for phenotypic-dependent interspecific competition. In both cases the survivorship of individuals of one population depends on the entire phenotypic distribution of the other species. The first model considers a continuously varying metric trait, with assortative or random mating; the second model examines a character controlled by two alleles at a single locus. Pursuing the notion that each population maximizes its mean fitness we define a vector-optimum strategy using the concepts of cooperative and competitive optima. It is found that the dynamical constraints placed on the equations of motion by Mendelian genetics often prevent a population from evolving to a strategic optimum. However, for the single locus case with complete dominance, the competitive optimum always coincides with some dynamical equilibrium on the Hardy-Weinberg manifold.

Alleles↗

Glycated haemoglobin centiles: a basis for audit in children's clinics?

Services for children with diabetes are a good model for audit because of relatively well-defined and available measures of outcome. Estimation of glycated haemoglobin (HbA1) is accepted as one index of control, although the assay and normal ranges vary in different centres. Sheffield has a stable clinic population and we have taken blood for HbA1 on each patient two to four times per year since 1983. HbA1 values have been aligned in 3-monthly increments from time of diagnosis to construct a graph showing the mean plus or minus two standard deviation values for the clinic population and also a rough 'centile chart' that describes the unfortunate but expected pattern of deteriorating control with duration of diabetes. There are undoubtedly methodological flaws in this novel approach; however, such charts constructed for each clinic may be a suitable basis for comparative assessment of the control of an individual and also for auditing the effects of changes in practice that strive for optimum glucose levels in the clinic population.

Age Factors↗

Lactose variability of Escherichia coli in thermally stressed reactor effluent waters.

Lactose-utilizing and nalidixic acid-resistant populations of Escherichia coli, having an optimum growth temperature of 37 degrees C, were placed in modified diffusion chambers. The chambers were submerged in the epilimnion and hypolimnion of a 1,100-hectare lake (Par Pond) which receives cooling water from a nuclear production reactor. Control chambers were placed in a deep-water reservoir and a Flowing-Streams Laboratory, both of which had comparable temperatures to Par Pond. The populations of E. coli were sampled regularly for up to 3 weeks. Viability of the bacteria was determined by dilution plating to nutrient agar followed by replicate plating onto selective medium to determine lactose utilization and nalidixic acid sensitivity. Initial populations of E. coli were lactose positive but changed to lactose negative in Par Pond when the reactor was operating (i.e., cooling water from the heat exchangers was being discharged to the lake). This alteration occurred most rapidly in the chambers closest to the cooling-water discharge point. Such changes did not occur in a deep-water reservoir, in Par Pond when the reactor was not operating, or in the Flowing-Streams Laboratory. The nalidixic acid-resistant characteristic remained stable regardless of the chambers' placement or reactor operations. Although the reasons for such alterations are unclear, it appears that lactose-negative populations of E. coli are selected for in these reactor effluent waters. The loss of the lactose characteristic prevents the recognition and identification of E. coli in this cooling lake (when the reactor is operating) and may prevent the assessment of water quality based on coliform recognition.

Drug Resistance, Microbial↗