Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Lymphatic System”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 181 records · Page 10Linked to original sources
PubMed2004

The lymphatic system in the dorsal skinfold chamber of the Syrian golden hamster in vivo.

The lymphatic network contributes to maintaining tissue homeostasis and immunological function by transporting fluid, plasma protein and cells from peripheral tissue via the lymph nodes into the blood vascular system. In contrast to the blood circulatory system, little is known about the lymphatic system. In particular, suitable animal models are lacking. Therefore, the dorsal skinfold chamber model was used to investigate the existence of a lymphatic system. To analyze the lymphatic network Syrian golden hamsters (n=12) fitted with titanium chambers were used. FITC-dextran of different concentrations (5% or 25%) and different molecular weights (4, 40 or 150 kDa) was used to contrast lymphatic vessels and measure initial lymph flow velocity. Intravital fluorescence microscopy enabled the quantification of diameter, velocity and branching order. Histology and electron microscopy supported the in vivo findings. Immediately after intradermal injection of FITC-dextran the lymphatics including valves were visible. The diameters of the lymphatic vessels (n=189) ranged from 133+/-5.4 microm (branching order 1) to 26+/-4.0 microm (branching order 5). Using different molecular weights of FITC-dextran, no significant differences in velocity were measured (327+/-157 microm/s with 4 kDa, 391+/-126 microm/s with 40 kDa, and 378+/-175 microm/s with 150 kDa). Blood and lymphatic vessels could not be differentiated clearly by H&E staining. However, endothelial cells of vessels with an irregularly shaped lumen containing no erythrocytes in cross section showed a weaker signal for CD31 staining as compared to endothelial cells of vessels containing erythrocytes. Moreover, transmission electron microscopy identified the dye-containing vessels as lymphatics after intradermal injection of Berlin Blue. In conclusion, a lymphatic network was characterized in the dorsal skinfold chamber model of the Syrian golden hamster. Thus, this well-established animal model for intravital microscopy provides the opportunity to elucidate the physiological and pathological function of the lymphatic vascular system.

Animals↗
PubMed1987

Role of the lymphatic system in the transport of absorbed 7,12-dimethylbenzanthracene in the rat.

To determine the role of the intestinal lymphatic system in the absorption of a polycyclic aromatic hydrocarbon, 7,12-dimethylbenzanthracene, a radiolabeled preparation of the compound was given by intraduodenal infusion to rats in doses of 10 micrograms, 10 mg and 20 mg in olive oil solution. The hydrocarbon appeared to be absorbed from the intestine in a fractional manner, ca. 20% of the administered radioactivity being recovered totally in bile and intestinal lymph in 24 hr at all three dose levels. Biliary radiolabel accounted for 75-82% of combined recovery of radioactivity in bile and lymph with all three doses. The recovery of significant amounts of radiolabel in bile before the appearance of isotope in lymph, together with the fact that the biliary radiolabel greatly exceeded at all times the lymphatic recovery of isotope, suggests that an alternative pathway, presumably the portal venous route, is of major importance in the transport of the absorbed hydrocarbon.

9,10-Dimethyl-1,2-benzanthracene↗
PubMed1995

[Lymphatic system--topographic and functional considerations contributing to complications in neck surgery].

During neck surgery some complications can involve the lymphatic system as well. The injury of the thoracic duct is the most important. A case of chylorrhoea after left radical neck dissection, which had been done by reason of metastases of larynx carcinoma, is reported. Chylorrhoea had yielded after underpin and ligation of the thoracic duct. Four weeks after radical neck dissection extensive tumor in postoperative scar had been found, and two weeks later numerous tumors in scar, on the neck, chin, face and chest had appeared. Patient had died ten weeks after neck dissection among the symptoms of cancerous cachexia.

Chylothorax↗
PubMed2001

LYVE-1, the lymphatic system and tumor lymphangiogenesis.

Previous research into hyaluronan (HA) has focused on the role of this abundant tissue glycosaminoglycan in promoting cell migration through interactions with its transmembrane receptor CD44 on inflammatory leukocytes and tumor cells. The recent discovery of a new HA receptor, LYVE-1 (lymphatic vessel endothelial HA receptor), expressed predominantly in lymphatic vessels, highlights another aspect of HA biology: its continuous transit through the lymphatic system and its potential involvement in lymph node homing by CD44+ leukocytes and tumor cells. The functional role of LYVE-1 in lymphatic vessels and its application as a marker to study tumor lymphangiogenesis are important areas of investigation.

Animals↗
PubMed1999

Size- and surface-dependent uptake of colloid particles into the lymphatic system.

PURPOSE: To examine the effect of particle size and surface characteristics on colloidal particle uptake from subcutaneous tissue into the lymphatic system. MATERIALS AND METHODS: Perfluorocarbon emulsion ZY13163 (median particle diameter, 0.08 micron; surfactant, egg yolk phospholipid), ZY13164 (median particle diameter, 0.36 micron; surfactant, egg yolk phospholipid), ZY13199 (0.18 micron, surfactants Tetronic904) and ZY14001 (0.11 micron, surfactant Pluronic L121) were individually injected into the dorsal skin of the hind foot of rabbits. Lymph flow rates and particle concentrations were determined in prenodal lymph fluid after injection, with and without massage of the skin over the injection site. RESULTS: In the first 24 hours after injection, the decreasing order of colloid flux without massage was as follows: ZY13199 > ZY14001 > ZY13163 > ZY13164. Lymph flow rates, lymph leukocyte concentrations and colloid concentrations increased substantially with mechanical skin massage. CONCLUSIONS: Transport of colloids into lymphatic vessels depends on particle size and surface characteristics. Without massage, colloids with egg yolk phospholipid exhibit size dependent decrease in uptake into lymphatics, while with massage only a weak correlation with particle size is present.

Animals↗
PubMed1985

[Role of the lymphatic system in the transport of biologically active substances in shock of various etiologies].

The content of histamine, serotonin, adrenaline and noradrenaline in the thoracic and lymphatic duct lymph and blood as well as absolute quantity of lymph transported biogenic amines and mediators into the general circulation were studied on 68 dogs during anaphylactic and endotoxin shock (ASh and ESh, respectively). Both ASh and ESh were accompanied by considerable changes in the hemodynamics, lymph circulation, content of biologically active substances in lymph and blood and in their lymph transport to the blood stream. The most profound and early changes in the content of biologically active substances during ASh and ESh were found in the lymph, which shows an important role of the lymphatic system in their resorption and transport from organs and tissues into the general circulation.

Anaphylaxis↗
PubMed1995

Mechanism of colloidal particle uptake into the lymphatic system: basic study with percutaneous lymphography.

PURPOSE: To examine the extracellular (dispersed particles) and intracellular (phagocytosed particles) colloidal particle transport from subcutaneous tissue into the lymphatic system. MATERIALS AND METHODS: Perfluorocarbon emulsions LA11063 (mean particle diameter, 0.34 micron) and ZY12149 (mean particle diameter, 0.06 micron) were individually injected into the dorsal skin of the hind foot of rabbits. Lymph flow rates and particle concentrations were determined in prenodal lymph fluid after injection, with and without massage of the skin over the injection site. RESULTS: In the first 24 hours after injection, extracellular colloid flux was substantially larger than intracellular flux. Lymph flow rates and colloid concentrations increased substantially with massage. Extracellular LA11063 flux was smaller than ZY12149 flux, whereas intracellular LA11063 flux exceeded ZY12149 flux. CONCLUSION: Transport of colloids into lymphatic vessels is dependent on particle size. Both extracellular and intracellular pathways are utilized. External tissue movement contributes substantially to the rate of particle uptake via both pathways.

Animals↗
PubMed1984

[Functional and topographic anatomy of the lymph heart and lymphatic system in the pelvic region of water-fowl and ratite birds].

The topography of the lymph system and of the lymph heart can be determined by injections of India ink, Berlin blue, Latex or contrast media. For the direct injection of marker medium into the lymphatic system of adult male birds, the lymphatic copulatory organ was chosen. In embryos and chicks, the foot pads are suited for an indirect injection of marker medium. Following the injections, the marker medium was passed on to the lymph heart via the lymph vessels of the pelvic limbs and by the truncus thoracoabdominalis. Efferent lymph vessels of the lymph heart empty into the pelvic venous system and into the lymphovenous sinus durae matris. The lymphovenous sinus durae matris is also suited for the injections to visualize the lymph heart. The formation of lymph and the course it takes to erect the lymphatic copulatory organ is described.

Animals↗