Annual warble fly survey, 1998/99.
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Between 1989 and 1997 different factors related to warble fly infestation (prevalence, intensity, climate, breed) were studied in north-eastern Algeria. Mean prevalence during the period was 76%. This figure was obtained from a cattle market survey of four different regions (Jijel, Constantine, Guelma, and El Tarf). One-hundred percent of the herds were found to be infested in El Tarf and 61% of animals were infested. Within a herd, the intensity of infestation decreased with the age of the cattle. The mean number of warbles per animal decreased as reinfestations occurred. The peak of the emergence of warbles observed between January and March was progressively delayed in the older animals. Two species of Hypoderma were present in cattle in Algeria (H. bovis and H. lineatum) with a predominance of H. lineatum (63%) in the semi-arid area (Batna) and a majority of H. bovis (75%) in humid area (Jijel). Warbles appeared earlier in Batna (October) than in Jijel (November). The Prim'Holstein breed was more susceptible to the warble fly infestation than Montbeliard or local breeds. The implementation of a control programme for hypodermosis in Algeria is urgent. Control can be achieved through a mass treatment of cattle. This approach must take into account the factors described in this study which influence the epidemiology of the disease.
The effect of the last developmental phase of the warble fly (Hypoderma bovis de Greer) larvas was studied as exerted on some health indices of milk in 20 experimental (treated) and 18 control (untreated) first-calvers of the Pinzgau breed at two localities of an area affected by bovine hypodermosis in the period from May to June, 1975. The preparation Hypocid, at a dose of 15 ml per 100 kg of body weight, was applied externally in the lumbosacral region to the experimental animals in November, 1974. Throughout the experimental period (61 days) the milk was repeatedly examined bacteriologically and cytologically, the content of chlorides and the milk electric conductivity were determined. In none of the followed health indices of milk were significant differences in the values obtained from experimental and control first-calvers proved statistically.
In two test areas in Austria (Burgenland, Lower Austria) ivermectin has been used to control oestrinosis and hypodermosis in red- and roe deer since many years (1985 and 1987, respectively). The results until 1992, using the injectable solution Ivomec-injection 1% only, have been reported (Kutzer, 1994). In continuation of these tests Ivomec-Praemix 0.6% was examined and it turned out that the treatment was extremely successful. In practice Ivomec-Praemix 0.6% has to be given preference due to its stability. The most favourable dosage for red- and roe deer was 2 x 0.4 mg ivermectin/kg bodyweight administered at an interval of one week, although equally good results could be obtained with 1 x 0.3-0.4 mg ivermectin/kg bodyweight. With the above mentioned dosages against Pharyngomyia picta and Cephenemyia stimulator an up to 100% and against Hypoderma actaeon a 100% success of treatment could be achieved. The administration in two doses has the advantage to eradicate intestinal and lung nematodes at the same time if there are any. Finally, it was found that the controlling measures should not be interrupted for more than one year, if no treatment was done in the neighbouring hunting grounds.
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An infestation with Hypoderma diana in roe deer in the Netherlands is described. The condition of the infested deer was poor. Hypoderma diana is no threat for cattle.
Immunological response to hypodermosis is studied by a passive hemagglutination method in three groups of cattle naturally infested during the spring of 1972. In the autumn, one group was treated with fenthion and another with niclofolan, a drug generally used for distomatosis, but also efficient in hypodermosis and free from side-effects. The third group served as a control. Two kinds of immunological response are observed after use of the insecticide. In the group treated with fenthion, there is a rapid drop in antibodies during the 3 days following treatment; this increases for the next 2 months up to a very low level, which is stable for 3 months (fig. 3, 4, 5). During the same period, antibodies in the control group increase regularly to reach a maximum in April or May (fig. 1, 2). The immunological response of the host is closely related to the antigenic stimulation of the larvae. In the group treated with niclofolan, the slow drop in antibodies suggests that there is a parallel drop in the number of larvae during the month following treatment. A study of the other group shows that larvae are affected in the 24 hours following fenthion administration. This agent, as well as all organophosphorous compounds, are well-known to act efficiently on larvae and have anaphylactic-type side-effects on the host. The massive degradation of larvae should have induced an important antigenic reaction, but no increase in the antibodies of the host is observed; there is a even rapid drop in the number of larvae immediately after treatment. This fact is investigated further by experiments on guinea pig: the role of antigen-antibody complexes is studied by passive anaphylaxis. It appears that shock reactions, following use of organophosphorous insecticides, have a true-hyper-sensitivity origin, and are increased by the intrinsic toxicity of the first instar larvae.
The migrating larvae of H. lineatum throughout the submucosal connective tissue of the oesophagus of cattle induce an acute inflammatory reaction. The aim of this study is to investigate histopathological tissue changes. Connective tissue damage is produced by the inflammatory reaction, but mostly by larval proteolytic enzymes. Fibrous proteins and matrix are all largely affected. Around the larvae there are 3 concentric levels of degradation. The external one is characterized by vascular response. No tissue component is destroyed, but the collagen fibers dissociate into fibrils. In the 2 internal levels (B, C) the fibrils lying along the larvae progressively degrade, splitting into large then small fragments. The elastic fibers are differently affected, but also show breaking and thinness. Finally, the matrix increases, metabhromatic reactions appear, and many mononuclear cells and granulocytes invade this injured area. Fibrin network generally present in inflammatory reaction is not found around the larvae except posteriorly in the regenerative zone. All the changes in the fibrous proteic structures of connective tissue facilitate larval migration. Repair in the larval pathway commences shortly after the passage of the larvae. Necrotic tissues are rapidly destroyed by larval enzymes and tissular and cellular enzymes, macrophages and granulocytes are abundant. The respective importance of larval enzymes and cellular enzymes in the necrotic phase is analyzed. This study, carried out between October and March, also shows an increase of inflammatory reaction and tissular degradation. The immune response of the host and the nutritional habits of the larvae are probably involved.
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Insect juvenile hormone mimics (IJH) at 0.1 and 0.01% were used as sprays for control of the short-nosed cattle louse, Haematopinus eurysternus Denny, on 11 heavily infested Hereford cows. A significant reduction of lice occurred although eradication was achieved in only one case. Severe reactions, apparently associated with the death or failure of complete development of cattle grub larvae, Hypoderma sp. Latr., were seen in the IJH-treated cows.
The investigation was carried out in Basilicata region (Southern Italy) from October 1997 to June 1998. Fifteen dairy cows bred in semiconfined conditions on a farm with a history of hypodermosis were sampled once a month for sera and milk; bulk milk from these animals was also collected monthly from the farm's tanker. Samples were tested for anti-Hypoderma spp. antibodies (Abs) with an ELISA technique and clinical parasitological examination was carried out monthly from January to July on all the animals in order to detect grubs. Blood and single and bulk milk samples yielded similar antibody kinetics and patterns in accordance with results obtained in previous immunological surveys in Italy. All animals were warbled in the spring time. November-January was confirmed to be the most suitable period for seroepidemiological survey for weather conditions in Southern Italy. The ELISA test proved once again to be very useful because it is simple to perform and cost effective. Either blood or milk samples may be used for epidemiological surveys; bulk milk may be very useful for the preliminary detection of hypodermosis on farms or in areas where there is no data available on the diffusion of the disease.
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