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Fermentation performance and intracellular metabolite patterns in laboratory and industrial xylose-fermenting Saccharomyces cerevisiae.

Heterologous genes for xylose utilization were introduced into an industrial Saccharomyces cerevisiae, strain A, with the aim of producing fuel ethanol from lignocellulosic feedstocks. Two transformants, A4 and A6, were evaluated by comparing the performance in 4-l anaerobic batch cultivations to both the parent strain and a laboratory xylose-utilizing strain: S. cerevisiae TMB 3001. During growth in a minimal medium containing a mixture of glucose and xylose (50 g/l each), glucose was preferentially consumed. During the first growth phase on glucose, the specific growth rates were 0.26, 0.32, 0.27 and 0.30 h(-1) for strains TMB 3001, A (parental strain), A4, and A6, respectively. The specific ethanol productivities were 0.04, 0.13, 0.04 and 0.03 g/g.per hour, for TMB 3001, A, A4 and A6, respectively. The specific xylose consumption rates were 0.06, 0.21 and 0.14 g/g.per hour, respectively for strains TMB 3001, A4 and A6. Xylose consumption resulted mainly in the formation of xylitol, with biomass and ethanol being minor products. The metabolite profile of intermediates in the pentose phosphate pathway and key glycolytic intermediates were determined during growth on glucose and xylose, respectively. The metabolite pattern differed depending on whether glucose or xylose was utilized. The levels of intracellular metabolites were higher in the industrial strains than in the laboratory strain during growth on xylose.

Anaerobiosis↗

[Comparative studies of the parameters of rumen fermentation and the digestibility of feed rations in cattle and sheep. 1. Parameters of rumen fermentation].

The results from 283 studies of rumen liquid, taken by means of oesophagus probe, from different species of cattle (ox, cow, male and female young cattle) and sheep (adult wether) are compared. In the rumen liquid the parts of volatile fatty acids (VFA), total VFA content, pH value and the content of NH3 and urea were analysed. In the cattle's rumen liquid the part of acetic acid is on average 2 Mol-% and the content of total VFA up to 28 mmol/l (middle of the cow--sheep comparisons) higher than in that of wether. The contents of NH3 and urea are lower in cattle than in wether. Relations between the amount of difference of characteristic rumen fluid numbers of cattle and sheep and the crude fibre content of the rations, the live weight of the young cattle and the nutrition level are considered.

Ammonia↗