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A new coccidian (Apicomplexa: eimeriidae) in the northern pocket gopher (Thomomys talpoides) and a comparison of oocyst survival in hosts from radon-rich and radon-poor soils.

Forty (93%) of 43 northern pocket gophers (Thomomys talpoides) from the Jemez Mountains, Sandoval County, New Mexico (USA), had coccidian oocysts in their feces when examined. We describe this parasite, Eimeria jemezi, n. sp. Sporulated oocysts were subspheroidal, 13.3 by 12.2 (10 to 17 by 9 to 15) microns, with sporocysts ellipsoidal, 7.1 by 4.4 (5 to 9 by 4 to 5) microns; micropyle and oocyst residuum were absent, but polar bodies, Stieda bodies and sporocyst residua were present. All gophers were collected from two sites of similar habitat 7 km apart. One site (R+) had a high soil radon content (> or = 50 to 70 picocuries (pCi) per liter of air) whereas the other site (R-) had soils that were near average natural levels (1.2 to 1.6 pCi/g uranium nucleotides per gram of soil; < 1.9 pCi/g thorium nucleotides). Twenty-one (88%) of 24 gophers from the R+ site had coccidian oocysts in their intestines when examined, but none of these oocysts ever sporulated, whereas all 19 (100%) gophers from the R- site had coccidian oocysts in their intestines and 16 (84%) of these samples sporulated normally under laboratory conditions. The elevated radon content of the soil may have had an adverse effect on the sporulation of this coccidian while it still was intracellular within its host.

Animals↗

Eimeria species (Apicomplexa: Eimeriidae) infecting Eliomys quercinus in an Alpine habitat.

Coccidian parasites were detected in an Alpine population of the garden dormouse (Eliomys quercinus), and 55-82% of the fecal samples collected during a two-year study (2000 and 2002) contained one or two eimerian species. We report the presence of Eimeria myoxi and confirm for the first time the presence of Eimeria melanuri in the garden dormouse. These Eimeria species can be considered common parasites of the garden dormouse and the Asian garden dormouse. The high prevalence might be due to group hibernation by the dormice.

Animals↗

Prevalence of Eimeria macusaniensis (Apicomplexa: Eimeriidae) in midwestern Lama spp.

To compare the prevalence of Eimeria macusaniensis among midwestern llamas (Lama glama), alpacas (Lama pacos), and guanacos (Lama guanicoe), feces were obtained from Lama spp. in 10 states between October 1989 and February 1996. Feces were examined by centrifugal flotation in sugar solution (specific gravity--1.28-1.30), and oocysts were quantified by a modified McMaster method. Data were compared by host species and age classifications. Typical oocysts occurred in samples from 28% of 76 herds and 10.4% of 443 animals including 12% of 301 llamas, 7% of 115 alpacas, and 7.4% of 27 guanacos. Prevalence was significantly greater (P = 0.009) in animals < 1 yr of age in comparison to older animals for llams (22.1 v.s. 8.5%) and for all Lama spp. combined (17.1 vs. 8.4%). Fecal oocyst abundance was significantly greater (P = 0.001) in llamas < 1 yr of age in comparison to older llamas (30 vs. 16 oocysts per g of feces). Fecal oocyst intensities did not differ significantly. Prevalence in both age groups of midwestern llamas was greater than previously reported for llamas in the western United States. Prevalence in midwestern alpacas < 1 yr of age was lower than reported for alpacas of similar age in South America, but oocyst intensities were similar. These results indicate that infection with E. macusaniensis is more common in Lama spp. in North America than previously recognized.

Animals↗

Descriptions of two new species of coccidia (Protozoa: Eimeriidae) and redescriptions of Eimeria ivensae and Eimeria odocoilei from captive white-tailed deer, Odocoileus virginianus.

Two new species of Eimeria were observed in the feces of captive white-tailed deer fawns, Odocoileus virginianus, from Alabama. The first new species was easily recognized because of its small size. Sporulated oocysts are spherical, average 10.2 by 10.0 microm, and lack a micropyle and oocyst residuum. Oocysts contain a polar granule and elongate-ellipsoidal sporocysts that measure 6.7 by 3.1 microm. A Stieda body is present on the sporocysts. Oocysts were observed in the feces, and gamonts and oocysts were observed in the jejunum of a month-old fawn from Minnesota that died from enteritis due to this species. Oocysts of this small species were present in 5 of the 6 white-tailed deer fawns examined. Oocysts of a second new species are ellipsoidal and average 29.5 by 24.6 microm. The oocyst encloses an oocyst residuum, polar granule, and elongate-ellipsoidal sporocysts that average 16.0 by 9.0 microm. A Stieda body and substieda body are present on the sporocysts. Oocysts of the second new species were present in 4 of the 6 white-tailed deer fawns examined. Oocysts of E. ivensae are ovoid or flask-like and average 32.0 by 20.8 microm. The oocyst wall is rough, contains a micropyle, and encloses elongate-ellipsoidal sporocysts that average 16.5 by 7.8 microm. A Stieda body is present on the sporocysts. Oocysts of E. ivensae were present in 4 of the 6 white-tailed deer fawns. Oocysts of E. odocoilei are spherical or slightly subspherical and measure 24.7 by 21.5 microm. They enclose ovoid sporocysts that average 12.7 by 8.8 microm. A Stieda and substieda body are present on the sporocyst. Oocysts of E. odocoilei were present in 4 of the 6 white-tailed deer fawns.

Animals↗

Eimeria Macyi sp. n. (Protozoa: Eimeriidae) from the eastern pipistrelle, Pipistrellus subflavus, from Alabama.

The subspherical to broadly ellipsoidal oocysts of Eimeria macyi measure 16 to 21 by 15 to 19 mu (mean 19.0 by 17.6 mu), and sporocysts measure 10 to 12 by 6 to 8 mu (mean 11.0 by 7.0 mu). The rough walled oocysts lack a micropyle and residuum, but a sporocyst residuum and polar granule are present. This is the first description of coccidian oocysts from the genus Pipistrellus.

Alabama↗

A new coccidian parasite (Apicomplexa: Eimeriidae) from the scimitar-horned oryx, Oryx dammah.

Oocysts of Eimeria oryxae sp. n. are described from the faeces of the scimitar-horned oryx, Oryx dammah (Cretzschmar, 1826), from Zoo Garden, Riyadh City, Saudi Arabia. Sporulated oocysts were ellipsoid in shape measuring 20.9 x 17.1 (16.7-24.2 x 15.5-20.2) microm, with smooth brownish-yellow double layered wall. Micropyle and ellipsoidal polar granules are present, but micropylar cap and oocyst residuum are absent. Sporocysts are ovoid, reaching 10.0 x 5.7 (9.2-11.0 x 5.2-6.5) microm with Stieda body and sporocyst residuum. Sporozoites are elongated, each with large and small refractile body.

Animals↗

The ultrastructural study of microgametogenesis of Eimeria scinci Phisalix 1923 (Apicomplexa: Eimeriidae) infecting the sandfish lizard, Scincus mitranus Anderson 1871 in Saudi Arabia.

The ultrastructure of microgametogenesis of Eimeria scinci Phisalix 1923 was described for the first time in the gall bladder epithelium of experimentally infected sandfish lizards, Scincus milranus Anderson 1871 from Al-Baha region in Saudi Arabia. Recorded sequence of events started as sexually differentiated second generation merozoites transformed into microgamonts, where most of the apicomplexan organelles have been disappeared gradually. Microgamonts were recognizable by the presence of peripherally arranged nuclei and the presence of one or two centrioles between each nucleus and the limiting membrane of the gamont. Early microgamonts were surrounded by a very narrow parasitophorous vacuoles, which widened during development and contained a few intravacuolar folds and tubules. Differentiation of microgametes began by elevations of the limiting membrane appeared above the centrioles and the segregation of nuclear content into a dense osmiophilic portion and an electron-pale one. A gradual protrusion of the dense portion of the nucleus and the developing flagella into the parasitophorous vacuole was proceeded. Fully developed microgametes become detached and occupied the parasitophorous vacuole along with the residual body of the mother microgamont. Each microgamete had at least two flagella; an anterior perforatorium; a dense elongate nucleus and an anteriorly located tubular mitochondrion. The (9x2+2) pattern of flagella was detected in transverse sections.

Animals↗

Eimeria chiropteri n.sp. (Apicomplexa: Eimeriidae) from Saudi Arabian bat Pipistrellus kuhlii (Chiroptera: Vespertilionidae).

Oocysts of Eimeria chiropteri n.sp. are described from faeces of Pipistrellus kuhlii from Saudi Arabia. The sporulated oocyst of E. chiropteri is broadly ellipsoidal 23.5 x 20.6 (18.8 - 25.9 x 16.5 - 25.2) microm, and bilayered wall 1.2 (1.1 - 1.3) microm thick. Micropyle is absent, but a single, spherical polar body present. Oocyst residuum is present as a coarse granular sphere. Sporocyst is ovoid 10.8 x 7.5 (10.1 - 11.7 x 6.8 - 8.1). Stieda body is present, but sub and parastieda bodies are absent. Sporozoites are elongated laying head to tail in the sporocyst each with a large spherical refractile globule. E. chiropteri is the 4th species from the genus Eimeria detected in bats of the genus Pipistrellus.

Animals↗

Eimeria juniataensis sp. n. (Protozoa: Eimeriidae) from the map turtle, Graptemys Geographica, in Pennsylvania.

Eimeria juniataensis sp. n. is described from the map turtle, Graptemys geographica, in Pennsylvania. The spherical to subspherical oocysts of E. juniataensis are 11.5 to 18.5 mu by 11.5 to 16.5 mu (mean, 13.5 by 12.9mu). The broadly fusiform sporocysts are 7.5 to 10 mu by 4 to 6.5 mu (mean, 8.3 by 5.0mu) and a Stieda body is present. Oocyst and sporocyst residua are present. This is the first report of an eimerian species from Graptemys.

Animals↗

Effects of eimerian (Apicomplexa: Eimeriidae) infections on nutrient assimilation in the Wyoming ground squirrel.

Effects of infection with mixed species of Eimeria (E. callospermophili, E. morainensis, and E. beecheyi) on the digestive physiology of Wyoming ground squirrels (Spermophilus elegans) are described. Infected and uninfected squirrels were administered arginine and methionine in saturated glucose solution. Blood was drawn at time 0 and 30 min postadministration. Significant differences were not found between infected and uninfected squirrels in plasma glucose, arginine, or methionine assimilation. In a second experiment, infected and uninfected squirrels were fed a food slurry of known caloric value. All feces were collected for 24 hr postfeeding. Differences were not detected in fecal caloric content or digestive efficiency. These results in conjunction with results reported in the literature suggest a reassessment of the "parasitic" nature of these squirrel symbionts. We propose that associations of some Eimeria species and hosts that evolve under natural conditions are examples of parasite-host interactions that often evolve toward commensalism.

Amino Acids↗

Fine structure studies of microgametogenesis of Eimeria adenoeides (Eimeriidae, Sporozoa) infecting turkeys in Egypt.

The Ultrastructure of microgametogenesis of Eimeria adenoeides was studied in the intestinal epithelium of experimentally infected turkeys' Meleagris gallopavo gallopavo. Microgamonts were recognizable by the presence of peripherally arranged nuclei and the presence of two centrioles between each nucleus and the limiting membrane of the gamont. A nuclear spindle apparatus and an intranuclear centrocone directed toward the centriole were observed. Each young microgamont was surrounded by a very narrow parasitophorous vacuole which widened during development and contained a few intravacuolar folds. Differentiation of the microgamete began when elevations of the limiting membrane appeared above the centrioles. This event was accompanied by the segregation of nuclear content into a dense osmiophilic portion and an electron-pale portion. A gradual protrusion of the dense portion of the nucleus and developing flagella into the parasitophorous vacuole was proceeded. Microgametes had an anterior perforatorium, a dense elongate nucleus, with an anteriorly positioned mitochondrion in a small groove of the nucleus. Usually two flagella could be detected per each mature microgamete.

Animals↗

Eimeria gasperettii n. sp. (Apicomplexa: Eimeriidae) from the horned viper, Cerates cerastes gasperettii in Saudi Arabia.

Eimeria gasperettii n. sp. is described from the gall bladder of the horned viper, Cerastes cerastes gasperettii from Al-Thumamah, central region of Saudi Arabia. Sporulated oocysts are ellipsoid in shape, measuring 38.8 x 25.5 (29-41 x 17.2-28.4) microm with smooth brownish-yellow bilayered wall, 1.7(1.5-2.0) microm.thick. Micropyle polar granule and oocyst residuum are absent. Sporocysts are ellipsoid reaching 13.8 x 9.6 (11-14.5 x 8.0-10.3) microm. Sporocyst residuum is present. The sporocyst lacks a Stieda body. Sporozoites are crescent-shaped blunt at one end and slightly tapered at the other. Eimeria species from Viperidae are compared.

Animals↗

Two new species of isospora (Apicomplexa: Eimeriidae) from geckoes of the genus Rhacodactylus (Sauria: Gekkonidae) endemic to New Caledonia.

Coprological examination of New Caledonian geckoes of the genus Rhacodactylus Fitzinger, 1843 revealed two new species of coccidia. Isospora leachiani sp. n. from R. leachianus (Cuvier, 1829) has oval, colourless oocysts, measuring 21-26 x 16-18.5 microm. Sporocysts are ellipsoidal, 11-12.5 x 6.5-8 microm, with distinct Stieda and substieda bodies. Oocysts of Isospora sykorai sp. n. from R. ciliatus (Guichenot, 1866) are elongately oval to cylindrical, 20-23.5 x 11-14 microm; sporocysts of this species are ellipsoidal, 10-11.5 x 7-8 microm, with a slightly pointed end and Stieda and substieda bodies. Infected geckoes did not exhibit any alteration of their health status.

Animals↗

[Species of the genus Eimeria Schneider, 1875 (Apicomplexa: Eimeriidae) in dairy goats maintained in intensive system in the São José do Rio Preto region, São Paulo State, Brazil].

Were examined 58 dairy goats, 41 kids and 17 adults, Saanen and Alpine breeds, males and females, in intensive system, in the São José do Rio Preto region, São Paulo state, for detection and identification of Eimeria spp. oocysts. The 58 feces samples analyzed by the Centrifugal-flotation technique, were positive for, at least, one Eimeria species. The Eimeria species found in this research were: E. ninakohlyakimovae (77,6%), E. jolchijevi (72,4%), E. alijevi (63,8%), E. christenseni (63,8%), E. arloingi (62,1%), E. caprovina (56,9%), E. hirci (50,0%) and E. caprina (48,3%). It follows that the high rate of the positive animals and the high frequency of the Eimeria species among the animals demonstrated that the disease is common in dairy goats, kids and adults, in intensive system.

Animal Husbandry↗