Testing for sterility of corn oil. I. Dispersion of spores in corn oil and in saline.
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The formation of zearalenone in a maize plot artificially infected with Fusarium culmorum was studied. The zearalenone concentration steeply increased only in the 8th week after inoculation and reached a maximum value of ca. 7 ppm, whereas zearalenone could not be detected in the control variants. The crude nutrient and dry matter content was not significantly influenced by the fungal infection. The infected crop showed average ear dry weights distinctly lower than that of the control variants (P less than or equal to 0.001). Apart from zearalenone, the mycotoxin deoxynivalenol was qualitatively detected in the infected maize. The toxicological relevance of the ascertained zearalenone content with regard to the health of dairy cattle and pigs was discussed.
The influence of silaging on zearalenone already formed and on the growth of Fusarium culmorum in naturally contaminated CCM maize was studied. The zearalenone content remained approximately constant (13.35 +/- 2.94 mg/kg, n = 59) over the whole test period (12 weeks), whereas Fusarium culmorum could not longer be detected after 11 days. Thus the hypothesis that zearalenone survives the silaging process unchanged is experimentally strongly supported. The study confirms the view that zearalenone detected in maize silages is probably already formed in the field or during intermediate storing. Relations between the fermentation process and the toxin content of the silage could not be ascertained.
The internal mycoflora of silage maize plants was examined in several years. Average Fusarium infection of the maize plants was 10.4% and leaf sheaths and stalks were preferably colonized by the fungi. During the growing season an increase in total colonization of the maize plant by field fungi as well as by species of the genus Fusarium was observed 17 different Fusarium species were isolated from silage maize plants. Most frequently occurring species (more than 85%) in descending order were F. avenaceum, F. culmorum, F. equiseti, F. sporotrichioides, F. crookwellense, F. oxysporum, F. sambucinum var. coeruleum.
The influence of physical treatment-expansion and flaking-on crude proteins degradability in the rumen was studied in maize, maize-gluten feed, rape extracted meal and in the expanded one at 120 degrees C and 150 degrees C, rape cake, wheat and flaked wheat by in sacco method. The enzymatic digestibility of crude protein in the rumen undegraded residues of the above mentioned feeds was determined by an enzymatically in vitro method. The treatment of feed decreased significantly the original solubility and theoretical degradability of crude proteins, and the amount of undegraded crude proteins was increased. Positive influence on the amount of enzymatically digested crude protein was determined in rape expanded at 120 degrees C and 150 degrees C (60, 61 and/or 68%). Flaking of wheat had a similar effect. Enzymatic digestibility at undegraded rests where increased by 8-10% after the heat treatment and it remained almost unchanged in expanded maize-gluten feed.
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