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PubMed · 9799763

Simplified interpretative format for assessing test interference: studies with hemoglobin-based oxygen carrier solutions.

S C Kazmierczak·P G Catrou·D Boudreau
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Abstract

Substances such as hemoglobin that interfere with analytical processes are recognized as a frequent source of error in laboratory medicine. Standard guidelines for assessment of test interferences assume that interference effects are not related to the concentration of the analyte being measured. However, previous investigations have demonstrated that interference effects can be markedly different, depending on the concentrations of interferent and analyte within the specimen. An experimental protocol for investigating these different types of interference effects has been developed. This protocol utilizes an orthogonally arranged matrix with progressively increasing concentrations of analyte and interferent. Evaluation of the measured analyte concentrations in specimens within the matrix using multiple regression analysis allows the magnitude, direction, and significance of each type of interference to be determined. Unfortunately, implementation of the interference data derived from the multiple regression analysis for judging the clinical acceptability of test results when an interferent is present is difficult. We describe a two-dimensional graphical format for evaluating the clinical acceptability of test results, based on criteria established under the Clinical Laboratory Improvement Amendments of 1988, in specimens containing hemoglobin-based oxygen carrier solutions.

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S C Kazmierczak, P G Catrou, D Boudreau. 1998. Simplified interpretative format for assessing test interference: studies with hemoglobin-based oxygen carrier solutions.. https://pubmed.ncbi.nlm.nih.gov/9799763/

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