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PubMed · 9667920

Preinitation complex assembly: potentially a bumpy path.

J M Cox·A R Kays·J F Sanchez·A Schepartz
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Abstract

During 1996 and 1997, several chemical issues that arise in the early stages of preinitiation complex (PIC) formation were resolved. Kinetics experiments indicated that both TBP dimerization and DNA bending influence the rate of TBP-TATA box assembly. Affinity cleavage experiments indicated that TBP lacks the specificity to nucleate assembly of a properly oriented PIC. Finally, high-resolution structures provided the atomic detail of early intermediates in PIC formation.

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DNADNA-Binding ProteinsHumansTATA BoxTATA-Box Binding ProteinTranscription FactorsTranscription, GeneticJournal ArticleResearch Support, U.S. Gov't, P.H.S.Review

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J M Cox, A R Kays, J F Sanchez, A Schepartz. 1998. Preinitation complex assembly: potentially a bumpy path.. https://doi.org/10.1016/s1367-5931(98)80030-6

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