Immunological screening method to detect specific translation products.

S Broome; W Gilbert

doi:10.1073/pnas.75.6.2746

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Immunological screening method to detect specific translation products.

S Broome·W Gilbert

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Abstract

We describe a very sensitive method to detect as antigens the presence of specific proteins within phage plaques or bacterial colonies. We coat plastic sheets with antibody molecules, expose the sheet to lysed bacteria so that a released antigen can bind, and then label the immobilized antigen with radioiodinated antibodies. Thus, the antigen is sandwiched between the antibodies attached to the plastic sheet and those carrying the radioactive label. Autoradiography then shows the positions of antigen-containing colonies or phage plaques. A few molecules of antigen released from each bacterial cell generatean adequate signal.

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Antibody Specificity Bacteriolysis Dose-Response Relationship, Immunologic Escherichia coli Galactosidases Immunologic Techniques Lactose Factors Lysogeny Protein Biosynthesis Radioimmunoassay beta-Galactosidase Journal Article Research Support, U.S. Gov't, P.H.S.

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S Broome, W Gilbert. 1978. Immunological screening method to detect specific translation products.. https://doi.org/10.1073/pnas.75.6.2746

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