Sequential CD34+ and CD4+ cell selection from leukapheresis components.

I J Webb; M Beach; J F Daley; D M Schott; K C Anderson

doi:10.1089/scd.1.1998.7.169

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Sequential CD34+ and CD4+ cell selection from leukapheresis components.

I J Webb·M Beach·J F Daley·D M Schott·K C Anderson

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Abstract

We tested the feasibility of sequential selection of CD34+ and CD4+ cell-enriched fractions from aliquots of five autologous leukapheresis components. CD34+ cells were selected from a median 8.0 x 10(8) mononuclear cells using the CellPro CEPRATE LC cell separation system. All cells in the CD34-depleted fraction (median 4.8 x 10(8), range 0.6-10.0 x 10(8) were then incubated with the appropriate antibodies for CD4+ cell selection and passed through a second LC column. Median target cell purities of the CD34+ cell-enriched fraction and CD4+ cell-enriched fraction were 90.5% and 86.0%, respectively. This study demonstrates that high purity CD34+ and CD4+ cell-enriched fractions can be isolated sequentially from leukapheresis components. In addition, CD8+ lymphocytes, implicated in graft-versus-host disease, were depleted in the course of both positive selection procedures. This approach could decrease the number of donor procedures by providing separate CD34(+)-enriched and CD4(+)-enriched populations for allogeneic peripheral blood progenitor cell transplantation and subsequent donor lymphocyte infusion from the same leukapheresis component.

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Antigens, CD34 CD4-Positive T-Lymphocytes Cell Separation Humans Leukapheresis Lymphocyte Depletion Journal Article

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I J Webb, M Beach, J F Daley, D M Schott, K C Anderson. 1998. Sequential CD34+ and CD4+ cell selection from leukapheresis components.. https://doi.org/10.1089/scd.1.1998.7.169

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