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PubMed · 929588

Decrease and recovery in cell surface charge induced by anesthetics.

K Sato·K Kojima·C Sato
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Abstract

We applied cell electrophoresis to detect the effect of anesthetics on membrane, and clarified the concentration - effect relationship and the term of reversibility. The cell electrophoretic mobility of rat erythrocytes decreased with time of incubation with intravenous anesthetics (thiopental or droperidol) at clinical concentrations and reached a minimum value of 25% reduction in 30 min. The effect changed gradually in the concentration range of 0.5-1000 microgram/ml of thiopental, and changed markedly with 0.3-1 microgram/ml of droperidol. Washing out of the anesthetics and subsequent incubation in serum-containing medium resulted in the complete recovery in the mobility when 5 microgram/ml thiopental or 1 microgram/ml droperidol was used. With high concentration of thiopental (100 microgram/ml) or droperidol (10 microgram/ml, however, unrecovered fraction of cells increased with time of treatment. The critical time for retaining the reversibility was about 2 hr.

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AnestheticsAnimalsCell MembraneCell MovementElectrophoresisErythrocytesIn Vitro TechniquesRatsSurface PropertiesThiopentalJournal Article

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K Sato, K Kojima, C Sato. 1977. Decrease and recovery in cell surface charge induced by anesthetics.. https://doi.org/10.1620/tjem.123.185

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