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PubMed · 9264021

Evidence for the complex formation between reverse transcriptase and multicopy single-stranded DNA in retron EC83.

D W Jeong·K Kim·D Lim
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Abstract

Multicopy single-stranded DNA (msDNA) is produced by reverse transcriptase (RT) and is mutagenic in E. coli. In order to study the interaction between msDNA and RT, an RT open reading frame of retron EC83 was fused with maltose binding protein. The fusion protein, MBP-RT, was active in msDNA synthesis. The msDNA was copurified with MBP-RT by amylose affinity column. When purified msDNA was mixed with MBP-RT, the msDNA formed a complex with the fusion protein. These results show that msDNA is present as a complex with RT in vivo and it is able to form such a complex in vitro. We suggest that RT may compete with MutS protein for binding to msDNA and thereby controls mutagenesis by msDNA.

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ATP-Binding Cassette TransportersAdenosine TriphosphatasesBacterial ProteinsBinding, CompetitiveCarrier ProteinsDNA, Single-StrandedDNA-Binding ProteinsEscherichia coliEscherichia coli ProteinsMaltoseMaltose-Binding ProteinsMonosaccharide Transport ProteinsMutS DNA Mismatch-Binding ProteinOpen Reading FramesProtein BindingRNA, BacterialRNA-Directed DNA PolymeraseRecombinant Fusion ProteinsRetroelementsJournal ArticleResearch Support, Non-U.S. Gov't

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D W Jeong, K Kim, D Lim. 1997-06-30. Evidence for the complex formation between reverse transcriptase and multicopy single-stranded DNA in retron EC83.. https://pubmed.ncbi.nlm.nih.gov/9264021/

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