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Thermal cycle dideoxy DNA sequencing.

Abstract

Thermal cycle dideoxy DNA sequencing eliminates the requirements for independent primer annealing and double-stranded DNA denaturation steps. The method enables sequencing from nanogram amounts of DNA from double-stranded and single-stranded PCR products, and plasmid or phage DNA templates. Thermal cycle sequencing also enables direct sequencing from bacterial colonies or phage plaques. Protocols using the Vent exo- DNA polymerase, helpful suggestions, and a troubleshooting guide are also presented.

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BibTeXRIS

B E Slatko. 1996. Thermal cycle dideoxy DNA sequencing.. https://doi.org/10.1007/bf02761709

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