PubMed · 8934949
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D Miller. 1995. Human gene transfer.. https://doi.org/10.1177/0895937495009003s1201
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Herpes simplex virus has been extensively genetically modified for gene transfer to nerve and other tissues, to create vectors that are devoid of viral gene expression and toxicity. Recombinant vectors have been engineered to express genes which protect neurons against toxic insults resulting in cell death, including nerve growth factor (NGF) and anti-apoptotic genes (eg bcl-2). This review describes experiments using HSV vectors expressing these gene products and their potential protective role in ameliorating neurodegenerative processes in animal model systems.
The potential of a genome-substituted form Avrolata (AABBUU) as a genetic system in genomic and chromosome manipulations for gene transfer from the wild species Aegilops umbellulata Eig. (UU) to cultivated wheat was studied. It was shown that plants combining resistance to leaf brown rust with high productivity may be produced from this form by classical hybridization procedures. The resistance gene introduced to line R-12 is dominant and probably identical to the Lr9 gene. By N-banding, chromosome staining technique and gliadin electrophoresis, the structural changes in chromosomes 1A, 2A, 4B, 6B, 7B, 1D, and 2D of the resistant line R-12 were revealed.
Lung cancer is the second cause of death after cardiovascular diseases and is the major cause of cancer deaths in the Western world. Large scale screening trials conducted 15-20 years ago using chest X-rays and sputum cytology were able to detect stage I cancers but failed to impact on survival. This is because of the early metastatic potential of small primary tumors. It is important then to detect lung cancer at an earlier stage, studying and identifying genetic lesions that could indicate a new target(s) for gene therapy. The retinoblastoma-related gene pRb2/p130, a new tumor suppressor gene cloned in 1993, is emerging as one of the candidate markers and targets for gene therapeutic approach. Effective genetic therapy requires both a genetic material to be used therapeutically and a means to deliver it. A scope for this review is to examine some of the gene delivery systems mostly used, discussing their weaknesses and strengths, and to discuss the role of pRb2/p130 in lung cancer.