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PubMed · 8893505

Decrease of intracellular fluorescein fluorescence polarization (IFFP) in human peripheral blood lymphocytes undergoing stimulation with phytohaemagglutinin (PHA), concanavalin A (ConA), pokeweed mitogen (PWM) and anti-CD3 antibody.

A Eisenthal·O Marder·D Dotan·S Baron·B Lifschitz-Mercer·S Chaitchik·R Tirosh·A Weinreb·M Deutsch
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Abstract

In the present study we describe the induction of changes in intracellular fluorescein fluorescence polarization (IFFP) in lymphocytes undergoing activation with a variety of stimulants. These stimulants included the lectins phytohaemagglutinin (PHA), concanavalin (ConA), pokeweed mitogen (PWM) and anti-CD3 antibody. Changes in IFFP were detected in individual cells using the Cellscan apparatus. Our results show that by employing mitogenic concentrations of PHA, as revealed in a [3H]-thymidine incorporation assay, a decrease in the IFFP in human peripheral blood lymphocytes (PBL) occurred within 40 min. ConA and anti-CD3 affected similarly IFFP, whereas PWM, a B lymphocyte lectin, had no effect on IFFP at the concentrations employed. Kinetic analysis revealed that changes in IFFP occurred within 20-40 min after exposure to the stimulants and lasted for 24 h. Our results show that stimulants which activate CD3+ lymphocytes caused immediate changes in IFFP, in an enriched population of human PBL. The possible mechanisms involved in IFFP modulation following exposure to selected stimulants are discussed.

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Antibody SpecificityCD3 ComplexConcanavalin ADose-Response Relationship, DrugFluoresceinFluoresceinsFluorescence PolarizationHumansKineticsLectinsLymphocyte ActivationLymphocytesMicroscopy, Electron, ScanningPhytohemagglutininsPokeweed MitogensJournal Article

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A Eisenthal, O Marder, D Dotan, S Baron, B Lifschitz-Mercer, S Chaitchik, R Tirosh, A Weinreb, M Deutsch. 1996. Decrease of intracellular fluorescein fluorescence polarization (IFFP) in human peripheral blood lymphocytes undergoing stimulation with phytohaemagglutinin (PHA), concanavalin A (ConA), pokeweed mitogen (PWM) and anti-CD3 antibody.. https://doi.org/10.1016/0248-4900(96)84778-2

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