Phage presentation.

H R Hill; P G Stockley

doi:10.1111/j.1365-2958.1996.tb02508.x

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PubMed · 8793867

Phage presentation.

H R Hill·P G Stockley

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Abstract

There has recently been great interest in the use of the filamentous bacteriophage fd as a vehicle for the display of peptides and proteins. Phage libraries displaying random peptides up to 38 amino acids in length can be used (i) to select for ligands able to bind specific target molecules; (ii) to mimic non-proteinaceous ligands; and (iii) as a tool to map epitopes recognized by antibodies. The display of proteins or their functional domains provides a system for the analysis of structure-function relationships, and the potential to generate proteins with altered binding characteristics or novel catalytic properties. The display of short immunogenic determinants on fusion phage may provide a basis for the development of novel peptide vaccines, whilst the expression of libraries of antibody fragments may provide a method to by-pass hybridoma technology in the generation of monoclonal antibodies.

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Capsid Capsid Proteins DNA, Circular DNA-Binding Proteins Epitopes Inovirus Proteins Structure-Activity Relationship Viral Fusion Proteins Viral Proteins Journal Article Review

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H R Hill, P G Stockley. 1996. Phage presentation.. https://doi.org/10.1111/j.1365-2958.1996.tb02508.x

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