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PubMed · 8743100

Decrease in high density lipoprotein cholesterol during prolonged storage. CELL Study Group.

T Ekbom·L H Lindholm·J Lanke·P Nilsson-Ehle
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Abstract

Different studies on the stability of high density lipoprotein cholesterol (HDL) in frozen serum or plasma have yielded conflicting results, namely increase, decrease, or no change at all during prolonged storage under freezing conditions. As part of a major trial on lipid-lowering strategies we statistically demonstrated a time-related decrease in HDL cholesterol during storage up to 46 months at -20 degrees C. We therefore re-analysed 85 frozen samples that had been analysed fresh and then stored from 26 to 46 months, using the dextran sulphate 500/Mg2+ method. A linear regression analysis of change in HDL cholesterol on time was performed. The slope was significantly negative (p < 0.0005). The regression equation was (decrease in HDL) = 0.05 - 0.008 x (time in months), i.e. after 6 months' storage at -20 degrees C there was almost a 1% decrease in the HDL cholesterol concentration per month of storage.

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Cholesterol, HDLCryopreservationHumansSpecimen HandlingTime FactorsTissue PreservationClinical TrialJournal ArticleMulticenter StudyRandomized Controlled TrialResearch Support, Non-U.S. Gov't

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T Ekbom, L H Lindholm, J Lanke, P Nilsson-Ehle. 1996. Decrease in high density lipoprotein cholesterol during prolonged storage. CELL Study Group.. https://doi.org/10.3109/00365519609088594

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Cholesterol, HDL↗