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PubMed · 8564304

Cytokinin induces a rapid decrease in the levels of mRNAs for catalase, 3-hydroxy-3-methylglutaryl CoA reductase, lectin and other unidentified proteins in etiolated cotyledons of cucumber.

T Toyama·H Teramoto·G Takeba·H Tsuji
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Abstract

Large-scale differential hybridization was performed to examine rapid changes in gene expression caused by a phytohormone, cytokinin, in etiolated cotyledons of cucumber (Cucumis sativus L.). We isolated 86 cDNA clones for mRNAs whose levels decreased within 2 h of the start of treatment with N6-benzyladenine (BA). Partial nucleotide sequences showed that some of the cDNAs were homologous to those for catalase, 3-hydroxy-3-methylglutaryl CoA reductase (HMGR) and a lectin. This is the first report that the levels of the mRNAs for those proteins are regulated by a cytokinin in darkness. Together with previous results [Teramoto et al. (1993) Physiol. Plant. 87: 584, (1994) Planta 193: 573, (1995) Planta 196: 387], the present study suggests that the cytokinin act to lower the levels of mRNAs transcribed from various genes in etiolated cotyledons.

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AdenineAmino Acid SequenceBase SequenceBenzyl CompoundsCatalaseCotyledonCucumis sativusCytokininsDNA, ComplementaryDNA, PlantGene Expression Regulation, PlantHydroxymethylglutaryl CoA ReductasesKinetinLectinsMolecular Sequence DataNucleic Acid HybridizationPlant Growth RegulatorsPlant LectinsPlant ProteinsPurinesRNA, MessengerRNA, PlantJournal Article

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T Toyama, H Teramoto, G Takeba, H Tsuji. 1995. Cytokinin induces a rapid decrease in the levels of mRNAs for catalase, 3-hydroxy-3-methylglutaryl CoA reductase, lectin and other unidentified proteins in etiolated cotyledons of cucumber.. https://pubmed.ncbi.nlm.nih.gov/8564304/

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