A counterselectable pACYC184-based lacZ alpha-complementing plasmid vector with novel multiple cloning sites; construction of chromosomal deletions in Klebsiella pneumoniae.

S Geissler; M Drummond

doi:10.1016/0378-1119(93)90474-h

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A counterselectable pACYC184-based lacZ alpha-complementing plasmid vector with novel multiple cloning sites; construction of chromosomal deletions in Klebsiella pneumoniae.

S Geissler·M Drummond

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Abstract

We have constructed a series of small, chloramphenicol-resistance-encoding, lacZ alpha-complementing vectors with novel multiple cloning sites, based on the pACYC184 replicon. The sacB gene of Bacillus subtilis, which is lethal to Gram- organisms in the presence of sucrose, was cloned into one of these, giving the counterselectable vector pSG335. This was used to substitute a streptomycin-resistance-encoding cassette for the ntrBC genes in the Klebsiella pneumoniae chromosome.

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Base Sequence Chloramphenicol Resistance Chromosome Deletion Chromosomes, Bacterial Cloning, Molecular DNA, Bacterial Genetic Complementation Test Genetic Vectors Hexosyltransferases Klebsiella pneumoniae Molecular Sequence Data Nitrogenase Plasmids Journal Article

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S Geissler, M Drummond. 1993-12-22. A counterselectable pACYC184-based lacZ alpha-complementing plasmid vector with novel multiple cloning sites; construction of chromosomal deletions in Klebsiella pneumoniae.. https://doi.org/10.1016/0378-1119(93)90474-h

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