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PubMed · 8203921

[MultiCycle software for cell cycle analyses].

S Funai·K Kurooka·Y Fujii·H Inufusa·H Yamada·Y Imanishi·S Sato·K Shindo·M Yasutomi
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Abstract

MultiCycle software (M-cycle), a computer cell cycle analysis program that has a background debris and aggregation compensating function, was utilized in this study to prove the usefulness of the M-cycle. The S phase fraction (SPF) calculated by the M-cycle was compared to that of bromodeoxyuridine labelling index (BLI) using colorectal carcinoma cell lines. The SPF value was slightly lower using the M-cycle than that of the BLI in Colo 201 and Colo 320 and lower significantly in Widr. This may indicate that the M-cycle effectively compensated for the background existing in the DNA histogram. The SPF value was computed both by the M-cycle and the sum of broadened rectangles model (SOBR). The SPF value of these cell lines showed a lower figure in the M-cycle than in the SOBR. The SPF value of paraffin-embedded material through the M-cycle and the SOBR was compared according to DNA ploidy patterns. The SPF value computed by the M-cycle was significantly lower in both ploidy patterns than that of the SOBR. In conclusion, the M-cycle is a useful tool for cell cycle analyses of simple DNA flow cytometric histograms obtained by paraffin-embedded material.

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Cell CycleColorectal NeoplasmsDNA, NeoplasmFlow CytometryHumansParaffin EmbeddingPloidiesS PhaseSoftwareEnglish AbstractJournal Article

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S Funai, K Kurooka, Y Fujii, H Inufusa, H Yamada, Y Imanishi, S Sato, K Shindo, M Yasutomi. 1994. [MultiCycle software for cell cycle analyses].. https://pubmed.ncbi.nlm.nih.gov/8203921/

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