PubMed · 8133395
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J R Salisbury, W F Whimster. 1993. Progress in computer-generated three-dimensional reconstruction.. https://doi.org/10.1002/path.1711700303
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Techniques have been developed for the routine reliable imaging of polysaccharides by atomic force microscopy (AFM). The polysaccharides are deposited from aqueous solution onto the surface of freshly cleaved mica, air dried, and then imaged under alcohols. The rationale behind the development of the methodology is described and data is presented for the bacterial polysaccharides xanthan, acetan, and the plant polysaccharides l-carrageenan and pectin. Studies on uncoated polysaccharides have demonstrated the improved resolution achievable when compared to more traditional metal-coated samples or replicas. For acetan the present methodology has permitted imaging of the helical structure. Finally, in addition to data obtained on individual polysaccharides, AFM images have also been obtained of the network structures formed by kappa-carrageenan and gellan gum.
In this article an analysis is performed of the results of stereogram construction using computer programs that model chemical compounds. Considerations about how best to represent models of molecules and improve legibility of stereograms are presented. An original diagrammatic substitute for the picture of a sphere, suitable for application in stereoscopic models of molecules, is proposed.
The principal components analysis has been applied to various imaging studies in nuclear medicine. This technical report describes a fast and accurate numerical method of calculating eigenvalues and eigenvectors in the principal components analysis dealing with larger image data sets. The method employs both data transformation and matrix transpose of original data sets to calculate a variance-covariance or correlation matrix. The method was tested on actual image data sets using a common workstation, confirming faster execution time and efficient accuracy in comparison to a standard method.