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PubMed · 8074877

Mixed-mode fluorescent DNA sequencing.

G De Bellis·I Consani·G Caramenti·R Pergolizzi·S Debernardi·L Invernizzi·M Luzzana
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Abstract

Fluorescence-based, automated DNA sequences represent one of the major advances in recent molecular biology. Two main technologies have been developed in this field: the single-label/four-lane system and the four-label/one-lane system. The following present the use of single-label-sequencing chemistry, which resembles traditional radioactive DNA sequencing, using the four-label system ABI 373A that expands its flexibility and obtains data that are immediately interpretable without software manipulation. This method has been named mixed-mode fluorescent DNA sequencing. Here we show one of its possible applications in molecular genetic analysis.

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AutoanalysisBase SequenceFluorescent DyesGlobinsHemoglobins, AbnormalHumansMolecular Sequence DataPolymerase Chain ReactionReagent Kits, DiagnosticSequence Analysis, DNASoftwareJournal ArticleResearch Support, Non-U.S. Gov't

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G De Bellis, I Consani, G Caramenti, R Pergolizzi, S Debernardi, L Invernizzi, M Luzzana. 1994. Mixed-mode fluorescent DNA sequencing.. https://pubmed.ncbi.nlm.nih.gov/8074877/

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