PubMed · 8043674
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M J Quinn, J A Hasbargen, B J Hasbargen. 1994. When does peritonitis occur?. https://pubmed.ncbi.nlm.nih.gov/8043674/
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Millions of people travel to developing countries, many of which are in the tropics, where the risk of becoming injured or sick is markedly increased and the access to appropriate medical treatment is markedly decreased. There are also common medical problems that all travelers confront, no matter what their itinerary. This article describes several traveling strategies for treating some common medical problems, lowering risk, and being able to get help should an emergency occur. Preparation is the watchword for the traveler.
In the maize endosperm, the Opaque2 (O2) basic leucine zipper transcriptional activator regulates the expression of a subset of the zein seed storage protein gene family. Immunodetection of wild-type or mutant O2 polypeptides fractionated by SDS-PAGE resolved a closely spaced doublet migrating in the 68- to 72-kD range, whereas by using isoelectric focusing, seven to nine isoforms were detected for each allele. Phosphatase treatment simplified the protein patterns to a single band corresponding to the nonphosphorylated component. In vivo and in vitro labeling confirmed that O2 can be phosphorylated. In protein gel blots probed with DNA, only the nonphosphorylated and hypophosphorylated O2 polypeptides were able to bind an oligonucleotide containing the O2 binding sequence. Upon in situ dephosphorylation of the focused isoforms by phosphatase treatment of the isoelectric focusing filter, the hyperphosphorylated forms acquired DNA binding activity. The ratio among the various isoforms remained constant throughout the developmental stages of endosperm growth but changed from daytime to nighttime, with a significant increase of the hyperphosphorylated forms during the night period. These results indicate that O2 exists in vivo as a pool of differently phosphorylated polypeptides and demonstrate that O2 DNA binding activity is modulated by a phosphorylation/dephosphorylation mechanism that appears to be influenced by environmental conditions.