PubMed · 7940232
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L Graabeck, L Therkelsen. 1994-03-16. [Quality development--databases as tool].. https://pubmed.ncbi.nlm.nih.gov/7940232/
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In conjunction with the King's Fund, Nursing Standard will be publishing regular reports on developments in nursing around the UK. Each report will focus on a specific area of practice and provide details of units which have experience in each area. The information will be based on material taken from the King's Fund network database and network days. To coincide with the launch of free network membership and to start the series, this report describes how the Nursing Developments Network is run.
Over 800 kbp of the 3-Mbp genome of Sulfolobus solfataricus have been sequenced to date. Our approach is to sequence subclones of mapped cosmids, followed by sequencing directly on cosmid templates with custom primers. Using a prototype automated system for genome-scale analysis, known as MAGPIE, along with other tools, we have discovered one open reading frame of at least 100 amino acids per kbp of sequence, and have been able to associate 50% of these with known genes through database searches. An examination of completely sequenced cosmids suggests a clustering of genes by function in the S. solfataricus genome.