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PubMed · 7924689

Fluorescence array detector for large-field quantitative fluorescence cytometry.

K D Wittrup·R J Westerman·R Desai
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Abstract

A prototypic fluorescence array detector (FAD) has been designed and constructed which is capable of quantifying single-cell fluorescence emissions from a statistically significant population of cell-sized objects (over 10(3)) on a solid substrate. The system is comprised of a cryogenically cooled CCD, 50 mW air-cooled argon ion laser, and optics that image a large (1 x 1 cm) field at 1:1 (no magnification). The CCD is effectively treated as a two-dimensional array of 2.7 x 10(5) independent 20 x 20 microns photodetectors, with each cell-sized object imaged across only a few CCD pixels. Algorithms have been developed for focusing, image segmentation, shading correction, and noise rejection; performance data for the FAD with fluorescent calibration beads are presented. The FAD is a simple alternative to microscope-based imaging cytometry, allowing large-field imaging without a scanning stage.

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Flow CytometryImage Processing, Computer-AssistedJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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K D Wittrup, R J Westerman, R Desai. 1994-07-01. Fluorescence array detector for large-field quantitative fluorescence cytometry.. https://doi.org/10.1002/cyto.990160304

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