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PubMed · 7897650

Thick bone section preparation using a silicon-rubber-based sealant.

H Cox·C Walker·C V Howard
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Abstract

A method has been developed, using a silicon-rubber-based sealant, which allows 2-3-mm-thick specimens to be maintained in a protected fluid environment for a number of months, without risk of dehydration. Following this, the specimen can be retrieved, stained, embedded and sectioned further. For example, 2-mm-thick sections of fixed unstained bone are easily examined by means of epi-illuminated polarized light and fluorescence microscopies using either conventional or confocal optics. The method could easily be extended to other tissues, for example brain tissue.

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H Cox, C Walker, C V Howard. 1995. Thick bone section preparation using a silicon-rubber-based sealant.. https://doi.org/10.1111/j.1365-2818.1995.tb03537.x

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