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PubMed · 7820282

Fluorogenic reactions for biomedical chromatography.

Y Ohkura·M Kai·H Nohta
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Abstract

A number of fluorogenic reactions, which have been used for HPLC detection systems by means of pre- and/or postcolumn derivatization, are surveyed with respect to both sensitivity and selectivity for the determination of biomedically important substances. For the derivatization of the substances, two types of fluorogenic reactions, fluorescence-generating and fluorescence-tagging, have been studied. The former are usable in most instances for both pre- and postcolumn derivatization methods, and the latter only for precolumn derivatization methods. HPLC methods utilizing the fluorogenic reactions allow analytes to be detected at picomole-subfemtomole levels. In the fluorescence-generating reactions, several fluorogenic reagents possessing two or more reactive sites in the molecule, which show molecular recognition for a variety of analytes, permit facile and reproducible detection in HPLC because there are fewer interferences from biological matrices.

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AminesAmino AcidsCarbohydratesChromatography, High Pressure LiquidFluorescent DyesNucleic AcidsPeptidesSpectrometry, FluorescenceSteroidsJournal ArticleReview

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Y Ohkura, M Kai, H Nohta. 1994-09-23. Fluorogenic reactions for biomedical chromatography.. https://doi.org/10.1016/0378-4347(94)00125-1

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