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PubMed · 7488168

Is polyamine decrease a common feature of apoptosis? Evidence from gamma rays- and heat shock-induced cell death.

E Grassilli·M A Desiderio·E Bellesia·P Salomoni·F Benatti·C Franceschi
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Abstract

Here we report that in rat thymocytes undergoing apoptosis upon two different stimuli, such as heat shock treatment and gamma irradiation, an early mRNA accumulation of ornithine decarboxylase (ODC)--the rate-limiting enzyme of polyamine biosynthesis--was followed by a very marked increase in ODC activity (28-40 and 6-8-fold, respectively). However, polyamine levels started to decrease before the appearance of DNA laddering, being putrescine and spermidine strongly diminished (8-12 hs), and spermine even depleted (12 hs). Taken together with our previous data on another model of apoptosis, i.e., glucocorticoid-induced cell death (Desiderio et al., Cell Growth Differ. 6: 505-513, 1995), these results suggest that an imbalance of polyamine metabolism, i.e., a strong activation of ODC and a paradoxical decrease of the intracellular polyamine content, might be a general feature of the apoptotic process.

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Analysis of VarianceAnimalsApoptosisCell DeathCells, CulturedChromatography, High Pressure LiquidDNAElectrophoresis, Agar GelGamma RaysHot TemperatureKineticsOrnithine DecarboxylasePolyaminesPutrescineRatsRats, Sprague-DawleySpermidineSpermineThymus GlandTime FactorsJournal ArticleResearch Support, Non-U.S. Gov't

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E Grassilli, M A Desiderio, E Bellesia, P Salomoni, F Benatti, C Franceschi. 1995-11-13. Is polyamine decrease a common feature of apoptosis? Evidence from gamma rays- and heat shock-induced cell death.. https://doi.org/10.1006/bbrc.1995.2679

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