Search PubMedSearch

PubMed · 72053

Bacterial gram staining by conventional and strip methods.

Abstract

Conventional gram staining was compared with a method which uses dye impregnated paper strips for staining. The paper strip technique was judged accurate and reproducible and is recommended for use in the small laboratory and hospital ward.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

N M Burdash, C E Bennett, A B Glassman. 1977. Bacterial gram staining by conventional and strip methods.. https://pubmed.ncbi.nlm.nih.gov/72053/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

[The bacteriological diagnosis of pneumonia by transtracheal puncture. Value in medical intensive care (author's transl)].

Seventy five patients referred with a diagnosis of pneumonia underwent transtracheal puncture. In 76% of cases this examination led to discovery of an organism in infected patients. In 50% of cases, the bacteriological diagnosis was sufficiently accurately oriented by direct examination to permit rapid and effective treatment. There was virtually perfect agreement with the results of blood cultures when the latter were positive. The organisms most often responsible were Gram positive and above all the pneumococcus. These results led to narrow spectrum antibiotic treatment appropriate for the organism. This early treatment, based upon the results of tracheal puncture, was associated with a favourable course in 85% of cases, in particular in severely ill patients requiring intubation. No notable complications occurred.

Bacteriological Techniques

Laboratory diagnosis in the problem of suspected gonococcal infection.

Proper laboratory techniques are indispensable for the diagnosis and detection of gonorrhea. While the smear is efficacious in diagnosing symptomatic urethral gonorrhea in men, it is not reliable for cervical, rectal, pharyngeal, or asymptomatic urethral infection. We have seen the development of four systems of culturing geared toward the office practitioner or where a complete laboratory is not available. They are as follows: Transgrow (transport), Neigon JEMBEC (transport or nontransport), Isocult, and Microcult systems. The Neigon JEMBEC system offers a good choice for the office practitioner. The use of proper diagnostic and culturing techniques and routine screening should bring about an increase in the amount of gonorrhea detected, treated, and subsequently removed from the reservoir of infection.

Bacteriological Techniques