Search PubMed⌕ Search
Home/Archive/PubMed

PubMed · 7166311

Apolipoprotein A-IV polymorphism in man.

H J Menzel·P M Kövary·G Assmann
View original source ↗Export metadata ↓

Abstract

In man, apolipoprotein A-IV is characterized by a genetically determined polymorphism controlled by two codominant alleles. Two isoforms of this apolipoprotein, designated A-IV-1 and A-IV-2, can be identified by isoelectric focusing. Among 1000 healthy factory workers participating in an epidemiological study, A-IV-1 (genotype 1-1) was observed in 85%; A-IV-2 (genotype 2-2), in 0.5%; and A-IV-1 in combination with A-IV-2 (genotype 1-2), in 14%. In four nonrelated subjects, an apolipoprotein A-IV variant (A-IV-Münster), characterized by a slightly more basic isoelectric focusing behavior than A-IV-2, was detected in combination either with A-IV-1 or A-IV-2. Mendelian inheritance of this variant could be demonstrated.

Explore related subjects

ApolipoproteinsApolipoproteins AHumansIsoelectric FocusingPhenotypePolymorphism, GeneticJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

H J Menzel, P M Kövary, G Assmann. 1982. Apolipoprotein A-IV polymorphism in man.. https://doi.org/10.1007/bf00304554

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed1995

Arachidonic acid binds to apolipoprotein D: implications for the protein's function.

The lipocalin apolipoprotein D (ApoD) is associated in human plasma with lecithin-cholesterol acyl transferase. It has also been found in high concentration in the fluid of gross cystic disease of the mammary gland. Using protein fluorescence quenching, it is shown that ApoD binds arachidonic acid (Ka of 1.6 x 10(8) M-1) and as previously known progesterone (Ka of 2.5 x 10(6) M-1), but neither cholesterol nor any of the other prostanoid molecules examined had measurable affinity. This specific binding of arachidonate, also observable directly, suggests a role for ApoD in the mobilisation of arachidonic acid, and hence prostaglandin synthesis.

Apolipoproteins↗
PubMed1995

Characterization of the apolipoprotein(a) gene.

Five homologous genes have been isolated and characterized and found to belong to the plasminogen-apolipoprotein(a) gene family. One of these genes is the true apolipoprotein(a) gene because it has typical regulatory elements and exons coding for kringle 4 repeats. Nucleotide sequence analysis revealed that each of 11 types of the kringle 4 repeats is encoded by two exons and that the splice junctions of the exons coding for the (pseudo)serine protease domain of apo(a) were also located in exactly the same positions as those of plasminogen.

Apolipoproteins↗
PubMed1995

Detection of polymorphisms in the 5'-flanking region of the gene for apolipoprotein(a).

Concentration of lipoprotein(a) in plasma is inherited in an autosomal co-dominant manner and its high concentration leads to atherothrombotic disease. Nucleotide sequence analysis of the apolipoprotein(a) gene revealed the presence of polymorphisms in its 5'-flanking region, which can be analyzed by in vitro amplification employing gene-specific primers. Genomic DNAs from normal individuals have been subclassified into four alleles according to the patterns of restriction digestion. The ratios of these subtypes differed between Caucasians and Japanese and in patients with myocardial infarction. It is very likely that the 5'-alleles in part control the plasma lipoprotein(a) level.

Apolipoproteins↗