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PubMed · 701817

Immune adherence: a quantitative and kinetic analysis.

T Sekine
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Abstract

Quantitative and kinetic analysis of the immune-adherence reaction (IA) between C3b fragments and IA receptors as an agglutination reaction is difficult. Analysis is possible, however, by use of radio-iodinated bovine serum albumin as antigen at low concentrations (less than 200 ng/ml) and optimal concentration of antibody to avoid precipitation of antigen--antibody complexes with human erythrocytes without participation of complement. Antigen and antibody are reacted at 37 degree C, complement is added, the mixture incubated and human erythrocytes added; after further incubation, ice-cold EDTA containing buffer is added and the erythrocytes centrifuged and assayed for radioactivity. Control cells reacted with heated guinea pig serum retained less than 5% of the added radioactivity. The method facilitates measurement of IA reactivity and permits more detailed analysis of the mechanism underlying the reaction.

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AntibodiesAntigen-Antibody ComplexComplement System ProteinsImmune Adherence ReactionKineticsSerum Albumin, BovineTime FactorsJournal Article

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T Sekine. 1978. Immune adherence: a quantitative and kinetic analysis.. https://doi.org/10.1016/0022-1759(78)90113-8

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