Search PubMed⌕ Search

PubMed · 6892169

Digital processing methods for structural analysis of an electron micrograph.

Abstract

Digital processing method using the scanning-densitometer with microcomputer provides a useful description system. It draws attention to the usefulness of local lattice averaging in clarifying images of periodic structures in crystals as well as crystalloids and para crystalline arrays in biological specimens. Digital processing of lattice images in crystal structures from the selected diffraction spots is successfully developed to the structural analysis of evaporated gold, graphitized carbon and human tooth enamel of hydroxyapatite. Furthermore, known microbody crystalloids in enzyme and icosahedral capsids in virus are confirmed and clarified by the procedure of selecting diffraction spectra transformed from the information taken with high resolution electron micrographs. The processed results can provide significant improvements in structural analysis of an electron micrograph.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

K Kanaya, N Baba, M Shino, T Ichijo, M Osumi, R W Horne. 1982. Digital processing methods for structural analysis of an electron micrograph.. https://pubmed.ncbi.nlm.nih.gov/6892169/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

Molecular replacement--historical background.

A review is given of the mathematical procedures required for a molecular-replacement structure determination. These apply equally to the more frequently encountered situations where a known homologous structure can be used as a search model and to phase determination in the presence of non-crystallographic symmetry (NCS). In general, the former represents improper NCS between two different unit cells, whereas the latter occurs when there is proper NCS within one unit cell.

Crystallography↗

Rotations and rotation matrices.

In molecular replacement, a model, described as a list of orthogonal coordinates, is to be moved into a new position and orientation. The orthogonal coordinate system also has to be related to the crystallographic system, which may not be orthogonal, and crystallographic symmetry must be considered, which applies to coordinates expressed as fractions of the unit cell. Elementary properties of rotation matrices and their representation as polar or Eulerian angles are discussed.

Crystallography↗

Implementation of molecular replacement in AMoRe.

An account is given of the molecular replacement method as implemented in the package AMoRe. The overall strategy of the method is presented and the main functions used in the package are described. The most important features of AMoRe are the quality of the fast rotation and translation functions and the facility of multiple inputs to translation and rigid-body refinement functions, which allow for a fast multiple exploration of crystal configurations with a high level of automation.

Crystallography↗