Mitochondrial DNA replication does not involve DNA polymerase alpha.

W Zimmermann; S M Chen; A Bolden; A Weissbach

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PubMed · 6777385

Mitochondrial DNA replication does not involve DNA polymerase alpha.

W Zimmermann·S M Chen·A Bolden·A Weissbach

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Abstract

Aphidicolin, a known inhibitor of DNA polymerase alpha, is a potent inhibitor of nuclear DNA synthesis in HeLa cells but has no effect on the replication of mitochondrial DNA. Parallel experiments with mitochondria incubated in vitro also show no inhibition of DNA synthesis by aphidicolin; however, DNA synthesis in these isolated mitochondria is completely blocked by dideoxycytidine triphosphate, which inhibits DNA polymerase gamma but not the alpha polymerase. The replication of mitochondrial DNA therefore requires only one DNA polymerase of the gamma type.

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Aphidicolin Cell Nucleus DNA Polymerase II DNA Polymerase III DNA Replication DNA, Mitochondrial DNA, Neoplasm Deoxyribonucleotides Diterpenes HeLa Cells Humans Kinetics Nucleic Acid Synthesis Inhibitors Journal Article

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W Zimmermann, S M Chen, A Bolden, A Weissbach. 1980-12-25. Mitochondrial DNA replication does not involve DNA polymerase alpha.. https://pubmed.ncbi.nlm.nih.gov/6777385/

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