Standardizing antimicrobial disk sensitivity testing.

K Faler

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PubMed · 6727855

Standardizing antimicrobial disk sensitivity testing.

K Faler

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Abstract

Before antimicrobial sensitivity testing, any pathogens must be isolated by streaking the clinical specimen on a plate of nutritive medium. Selecting a drug based on tests of a mixed culture from which a pathogen has not been isolated encourages unnecessary treatment. A plate of Mueller-Hinton agar is swabbed with an inoculum from a standardized broth culture. Drug-impregnated paper disks are applied to the plate at least 3 cm apart and at least 2 cm from the plate's edge. The zone of inhibition varies with each drug and is measured, after overnight incubation at 35-37 C, with the aid of over-the-shoulder lighting and a dark background.

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Aerobiosis Anti-Bacterial Agents Bacteria Microbial Sensitivity Tests Quality Control Journal Article

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K Faler. 1984. Standardizing antimicrobial disk sensitivity testing.. https://pubmed.ncbi.nlm.nih.gov/6727855/

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